Urinary Proteome and Exosome Analysis Protocol for the Discovery of Respiratory Diseases Biomarkers

Urinary Proteome and Exosome Analysis Protocol for the Discovery of Respiratory Diseases Biomarkers

This study aims to develop a protocol for respiratory disease-associated biomarker discovery by combining urine proteome studies with urinary exosome components analysis (i.e., miRNAs). To achieve this, urine was DTT treated to decrease uromodulin, then concentrated and ultracentrifuged. Proteomic a...

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Main Authors: Laura Martelo-Vidal, Sara Vázquez-Mera, Pablo Miguéns-Suárez, Susana Belén Bravo-López, Heidi Makrinioti, Vicente Domínguez-Arca, Javier de-Miguel-Díez, Alberto Gómez-Carballa, Antonio Salas, Francisco Javier González-Barcala, Francisco Javier Salgado, Juan José Nieto-Fontarigo
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Biomolecules
Subjects:
exosomes
miRNAs
proteomics
respiratory disease biomarkers
urine
Online Access:https://www.mdpi.com/2218-273X/15/1/60
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