Intensive cultivation of whiteleg shrimp (Litopenaeus vannamei) under biofloc condition in land-based ponds
Land-based pond systems, as an emerging intensive aquaculture model, have encountered developmental constraints due to toxic nitrogen levels. This study aimed to develop and evaluate a biofloc-mediated aquaculture system in land-based ponds, focusing on water quality, bacterial composition of bioflo...
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Elsevier
2025-04-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513425000766 |
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| author | Lu Jing Huan Ren Wujie Xu Haochang Su Xiaojuan Hu Guoliang Wen Yu Xu Lingjie Tu Yucheng Cao |
| author_facet | Lu Jing Huan Ren Wujie Xu Haochang Su Xiaojuan Hu Guoliang Wen Yu Xu Lingjie Tu Yucheng Cao |
| author_sort | Lu Jing |
| collection | DOAJ |
| description | Land-based pond systems, as an emerging intensive aquaculture model, have encountered developmental constraints due to toxic nitrogen levels. This study aimed to develop and evaluate a biofloc-mediated aquaculture system in land-based ponds, focusing on water quality, bacterial composition of bioflocs, shrimp growth performance, and nonspecific immunity. Juveniles of Litopenaeus vannamei were stocked in three identical land-based barrel tanks at a density of 600 individuals·m−2 for a 90-day cultivation period, with no water exchange. The results indicated that total ammonia nitrogen (TAN) concentrations were maintained between 0 and 4 mg·L−1, and nitrite (NO2--N) levels were kept between 0 and 1.0 mg·L−1. Bacterial community analysis revealed that the richness and Shannon index of bioflocs at 90 days were significantly higher, yet the dominant bacteria throughout the trial remained consistent, comprising Proteobacteria, Actinobacteria, Planctomycetes, Bacteroidetes, and Chloroflexi. Redundancy Analysis (RDA) demonstrated that Bacteroidetes were significantly positively correlated with TAN and NO2--N at 30 and 60 days, while Chloroflexi showed a positive correlation with nitrate (NO3--N) and phosphate (PO43-) at 90 days. Growth performance metrics indicated a survival rate (SR) of L. vannamei at (78.36 ± 1.81)%, yield of (8.63 ± 0.33) kg·m−3, specific growth rate (SGR) of (2.05 ± 0.04)%·d−1, feed conversion rate (FCR) of 1.53 ± 0.25, and water usage of (192.29 ± 9.01) L·kg−1 (shrimp). The relative expression levels of immune-related genes, including SOD, CAT, LZM, proPO, TLR2, and VEGF1L, exhibited an increasing trend over the course of the aquaculture process. Collectively, the findings suggest that bioflocs effectively regulated water quality and enhanced shrimp growth, production, and immune status. |
| format | Article |
| id | doaj-art-caaf43644e7c44f98936e8780da8974b |
| institution | DOAJ |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
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| series | Aquaculture Reports |
| spelling | doaj-art-caaf43644e7c44f98936e8780da8974b2025-08-20T03:05:53ZengElsevierAquaculture Reports2352-51342025-04-014110269010.1016/j.aqrep.2025.102690Intensive cultivation of whiteleg shrimp (Litopenaeus vannamei) under biofloc condition in land-based pondsLu Jing0Huan Ren1Wujie Xu2Haochang Su3Xiaojuan Hu4Guoliang Wen5Yu Xu6Lingjie Tu7Yucheng Cao8National Engineering Research Center for Marine Aquaculture, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, Zhejiang Province 316004, China; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization/ Ministry of Agriculture and Rural Affairs, Guangzhou 510300, ChinaSouth China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization/ Ministry of Agriculture and Rural Affairs, Guangzhou 510300, ChinaSouth China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization/ Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519082, ChinaSouth China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization/ Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519082, ChinaSouth China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization/ Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519082, ChinaNational Engineering Research Center for Marine Aquaculture, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, Zhejiang Province 316004, China; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization/ Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519082, ChinaSouth China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization/ Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China; Key Laboratory of Efficient Utilization and Processing of Marine Fishery Resources of Hainan Province, Sanya 572018, China; Corresponding author at: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization/ Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China.South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization/ Ministry of Agriculture and Rural Affairs, Guangzhou 510300, ChinaNational Engineering Research Center for Marine Aquaculture, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, Zhejiang Province 316004, China; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization/ Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519082, China; Corresponding author at: National Engineering Research Center for Marine Aquaculture, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, Zhejiang Province 316004, China.Land-based pond systems, as an emerging intensive aquaculture model, have encountered developmental constraints due to toxic nitrogen levels. This study aimed to develop and evaluate a biofloc-mediated aquaculture system in land-based ponds, focusing on water quality, bacterial composition of bioflocs, shrimp growth performance, and nonspecific immunity. Juveniles of Litopenaeus vannamei were stocked in three identical land-based barrel tanks at a density of 600 individuals·m−2 for a 90-day cultivation period, with no water exchange. The results indicated that total ammonia nitrogen (TAN) concentrations were maintained between 0 and 4 mg·L−1, and nitrite (NO2--N) levels were kept between 0 and 1.0 mg·L−1. Bacterial community analysis revealed that the richness and Shannon index of bioflocs at 90 days were significantly higher, yet the dominant bacteria throughout the trial remained consistent, comprising Proteobacteria, Actinobacteria, Planctomycetes, Bacteroidetes, and Chloroflexi. Redundancy Analysis (RDA) demonstrated that Bacteroidetes were significantly positively correlated with TAN and NO2--N at 30 and 60 days, while Chloroflexi showed a positive correlation with nitrate (NO3--N) and phosphate (PO43-) at 90 days. Growth performance metrics indicated a survival rate (SR) of L. vannamei at (78.36 ± 1.81)%, yield of (8.63 ± 0.33) kg·m−3, specific growth rate (SGR) of (2.05 ± 0.04)%·d−1, feed conversion rate (FCR) of 1.53 ± 0.25, and water usage of (192.29 ± 9.01) L·kg−1 (shrimp). The relative expression levels of immune-related genes, including SOD, CAT, LZM, proPO, TLR2, and VEGF1L, exhibited an increasing trend over the course of the aquaculture process. Collectively, the findings suggest that bioflocs effectively regulated water quality and enhanced shrimp growth, production, and immune status.http://www.sciencedirect.com/science/article/pii/S2352513425000766Litopenaeus vannameiWater qualityBiofloc bacteriaGrowth performanceGene expression |
| spellingShingle | Lu Jing Huan Ren Wujie Xu Haochang Su Xiaojuan Hu Guoliang Wen Yu Xu Lingjie Tu Yucheng Cao Intensive cultivation of whiteleg shrimp (Litopenaeus vannamei) under biofloc condition in land-based ponds Aquaculture Reports Litopenaeus vannamei Water quality Biofloc bacteria Growth performance Gene expression |
| title | Intensive cultivation of whiteleg shrimp (Litopenaeus vannamei) under biofloc condition in land-based ponds |
| title_full | Intensive cultivation of whiteleg shrimp (Litopenaeus vannamei) under biofloc condition in land-based ponds |
| title_fullStr | Intensive cultivation of whiteleg shrimp (Litopenaeus vannamei) under biofloc condition in land-based ponds |
| title_full_unstemmed | Intensive cultivation of whiteleg shrimp (Litopenaeus vannamei) under biofloc condition in land-based ponds |
| title_short | Intensive cultivation of whiteleg shrimp (Litopenaeus vannamei) under biofloc condition in land-based ponds |
| title_sort | intensive cultivation of whiteleg shrimp litopenaeus vannamei under biofloc condition in land based ponds |
| topic | Litopenaeus vannamei Water quality Biofloc bacteria Growth performance Gene expression |
| url | http://www.sciencedirect.com/science/article/pii/S2352513425000766 |
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