Assessing the Ethanologenic Potential of Xylose-Fermenting Yeasts Scheffersomyces stipitis UCM Y-2810
Background. Enhancing the efficiency of second-generation (2G) bioethanol production from lignocellulosic biomass is crucial for advancing sustainable biofuel technologies. However, the conversion of biomass into 2G bioethanol faces substantial challenges, necessitating a comprehensive investigatio...
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Igor Sikorsky Kyiv Polytechnic Institute
2025-04-01
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| Series: | Innovative Biosystems and Bioengineering |
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| Online Access: | https://ibb.kpi.ua/article/view/311267 |
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| author | Daria Antonenko Olena Mokrousova Anna Oleshko Olena Budyakova Ihor Hretskyi Marina Fomina |
| author_facet | Daria Antonenko Olena Mokrousova Anna Oleshko Olena Budyakova Ihor Hretskyi Marina Fomina |
| author_sort | Daria Antonenko |
| collection | DOAJ |
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Background. Enhancing the efficiency of second-generation (2G) bioethanol production from lignocellulosic biomass is crucial for advancing sustainable biofuel technologies. However, the conversion of biomass into 2G bioethanol faces substantial challenges, necessitating a comprehensive investigation of microbial agents.
Objective. To evaluate the effect of glucose and xylose concentrations, as well as cultivation duration, on the efficiency of ethanologenesis using the model organism Scheffersomyces stipitis UCM Y-2810, and to determine the optimal conditions for achieving maximum ethanol yield.
Methods. The effects of glucose and xylose concentrations and cultivation time on ethanologenesis efficiency were evaluated using S. stipitis UCM Y-2810 as a model organism. The experimental design included three levels of factors: xylose concentration (3, 16.5, and 30 g/l), glucose concentration (1, 5.5, and 10 g/l), and cultivation durations (1, 2, and 3 days). Statistical analysis of the experimental data was conducted using a three-factor, three-level Box–Behnken design.
Results. Under submerged cultivation of the strain of S. stipitis UCM Y-2810 in model media, optimization of the ethanologenesis process resulted in a maximum ethanol yield of 7.74 g/l. The optimal conditions for this yield were identified as follows: xylose concentration of 16.5 g/l, glucose concentration of 7.75 g/l, and a cultivation time of 3 days.
Conclusions. The application of the Box–Behnken design revealed that the statistically significant factors influencing ethanologenesis efficiency were xylose concentration, yeast cultivation duration, and the linear-quadratic interaction between these two factors.
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| format | Article |
| id | doaj-art-caaba9d1ee0a45adac2bd9b187f278c1 |
| institution | OA Journals |
| issn | 2616-177X |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Igor Sikorsky Kyiv Polytechnic Institute |
| record_format | Article |
| series | Innovative Biosystems and Bioengineering |
| spelling | doaj-art-caaba9d1ee0a45adac2bd9b187f278c12025-08-20T02:15:45ZengIgor Sikorsky Kyiv Polytechnic InstituteInnovative Biosystems and Bioengineering2616-177X2025-04-019110.20535/ibb.2025.9.1.311267Assessing the Ethanologenic Potential of Xylose-Fermenting Yeasts Scheffersomyces stipitis UCM Y-2810Daria Antonenko0Olena Mokrousova1Anna Oleshko2Olena Budyakova3Ihor Hretskyi4Marina Fomina5D.K. Zabolotny Institute of Microbiology and Virology of NAS of UkraineKyiv National University of Technologies and DesignKyiv National University of Technologies and DesignKyiv National University of Technologies and DesignD.K. Zabolotny Institute of Microbiology and Virology of NAS of Ukraine; Kyiv National University of Technologies and DesignD.K. Zabolotny Institute of Microbiology and Virology of NAS of Ukraine Background. Enhancing the efficiency of second-generation (2G) bioethanol production from lignocellulosic biomass is crucial for advancing sustainable biofuel technologies. However, the conversion of biomass into 2G bioethanol faces substantial challenges, necessitating a comprehensive investigation of microbial agents. Objective. To evaluate the effect of glucose and xylose concentrations, as well as cultivation duration, on the efficiency of ethanologenesis using the model organism Scheffersomyces stipitis UCM Y-2810, and to determine the optimal conditions for achieving maximum ethanol yield. Methods. The effects of glucose and xylose concentrations and cultivation time on ethanologenesis efficiency were evaluated using S. stipitis UCM Y-2810 as a model organism. The experimental design included three levels of factors: xylose concentration (3, 16.5, and 30 g/l), glucose concentration (1, 5.5, and 10 g/l), and cultivation durations (1, 2, and 3 days). Statistical analysis of the experimental data was conducted using a three-factor, three-level Box–Behnken design. Results. Under submerged cultivation of the strain of S. stipitis UCM Y-2810 in model media, optimization of the ethanologenesis process resulted in a maximum ethanol yield of 7.74 g/l. The optimal conditions for this yield were identified as follows: xylose concentration of 16.5 g/l, glucose concentration of 7.75 g/l, and a cultivation time of 3 days. Conclusions. The application of the Box–Behnken design revealed that the statistically significant factors influencing ethanologenesis efficiency were xylose concentration, yeast cultivation duration, and the linear-quadratic interaction between these two factors. https://ibb.kpi.ua/article/view/311267lignocellulosic biomassxylose-fermenting yeastsScheffersomyces stipites2G bioethanol |
| spellingShingle | Daria Antonenko Olena Mokrousova Anna Oleshko Olena Budyakova Ihor Hretskyi Marina Fomina Assessing the Ethanologenic Potential of Xylose-Fermenting Yeasts Scheffersomyces stipitis UCM Y-2810 Innovative Biosystems and Bioengineering lignocellulosic biomass xylose-fermenting yeasts Scheffersomyces stipites 2G bioethanol |
| title | Assessing the Ethanologenic Potential of Xylose-Fermenting Yeasts Scheffersomyces stipitis UCM Y-2810 |
| title_full | Assessing the Ethanologenic Potential of Xylose-Fermenting Yeasts Scheffersomyces stipitis UCM Y-2810 |
| title_fullStr | Assessing the Ethanologenic Potential of Xylose-Fermenting Yeasts Scheffersomyces stipitis UCM Y-2810 |
| title_full_unstemmed | Assessing the Ethanologenic Potential of Xylose-Fermenting Yeasts Scheffersomyces stipitis UCM Y-2810 |
| title_short | Assessing the Ethanologenic Potential of Xylose-Fermenting Yeasts Scheffersomyces stipitis UCM Y-2810 |
| title_sort | assessing the ethanologenic potential of xylose fermenting yeasts scheffersomyces stipitis ucm y 2810 |
| topic | lignocellulosic biomass xylose-fermenting yeasts Scheffersomyces stipites 2G bioethanol |
| url | https://ibb.kpi.ua/article/view/311267 |
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