Adaptive Bacteria Colony Picking in Unstructured Environments Using Intensity Histogram and Unascertained LS-SVM Classifier

Features analysis is an important task which can significantly affect the performance of automatic bacteria colony picking. Unstructured environments also affect the automatic colony screening. This paper presents a novel approach for adaptive colony segmentation in unstructured environments by trea...

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Main Authors: Kun Zhang, Minrui Fei, Xin Li, Huiyu Zhou
Format: Article
Language:English
Published: Wiley 2014-01-01
Series:The Scientific World Journal
Online Access:http://dx.doi.org/10.1155/2014/928395
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author Kun Zhang
Minrui Fei
Xin Li
Huiyu Zhou
author_facet Kun Zhang
Minrui Fei
Xin Li
Huiyu Zhou
author_sort Kun Zhang
collection DOAJ
description Features analysis is an important task which can significantly affect the performance of automatic bacteria colony picking. Unstructured environments also affect the automatic colony screening. This paper presents a novel approach for adaptive colony segmentation in unstructured environments by treating the detected peaks of intensity histograms as a morphological feature of images. In order to avoid disturbing peaks, an entropy based mean shift filter is introduced to smooth images as a preprocessing step. The relevance and importance of these features can be determined in an improved support vector machine classifier using unascertained least square estimation. Experimental results show that the proposed unascertained least square support vector machine (ULSSVM) has better recognition accuracy than the other state-of-the-art techniques, and its training process takes less time than most of the traditional approaches presented in this paper.
format Article
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language English
publishDate 2014-01-01
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record_format Article
series The Scientific World Journal
spelling doaj-art-caa96c4144a24ed0a57a32725d3535cb2025-08-20T02:19:35ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/928395928395Adaptive Bacteria Colony Picking in Unstructured Environments Using Intensity Histogram and Unascertained LS-SVM ClassifierKun Zhang0Minrui Fei1Xin Li2Huiyu Zhou3School of Mechatronic Engineering & Automation, Shanghai University, M8 Building, 149 Yanchang Road, ZhaBei District, Shanghai 200072, ChinaSchool of Mechatronic Engineering & Automation, Shanghai University, M8 Building, 149 Yanchang Road, ZhaBei District, Shanghai 200072, ChinaSchool of Mechatronic Engineering & Automation, Shanghai University, M8 Building, 149 Yanchang Road, ZhaBei District, Shanghai 200072, ChinaThe Institute of Electronics, Communications and Information Technology, Queen’s University Belfast, Belfast, UKFeatures analysis is an important task which can significantly affect the performance of automatic bacteria colony picking. Unstructured environments also affect the automatic colony screening. This paper presents a novel approach for adaptive colony segmentation in unstructured environments by treating the detected peaks of intensity histograms as a morphological feature of images. In order to avoid disturbing peaks, an entropy based mean shift filter is introduced to smooth images as a preprocessing step. The relevance and importance of these features can be determined in an improved support vector machine classifier using unascertained least square estimation. Experimental results show that the proposed unascertained least square support vector machine (ULSSVM) has better recognition accuracy than the other state-of-the-art techniques, and its training process takes less time than most of the traditional approaches presented in this paper.http://dx.doi.org/10.1155/2014/928395
spellingShingle Kun Zhang
Minrui Fei
Xin Li
Huiyu Zhou
Adaptive Bacteria Colony Picking in Unstructured Environments Using Intensity Histogram and Unascertained LS-SVM Classifier
The Scientific World Journal
title Adaptive Bacteria Colony Picking in Unstructured Environments Using Intensity Histogram and Unascertained LS-SVM Classifier
title_full Adaptive Bacteria Colony Picking in Unstructured Environments Using Intensity Histogram and Unascertained LS-SVM Classifier
title_fullStr Adaptive Bacteria Colony Picking in Unstructured Environments Using Intensity Histogram and Unascertained LS-SVM Classifier
title_full_unstemmed Adaptive Bacteria Colony Picking in Unstructured Environments Using Intensity Histogram and Unascertained LS-SVM Classifier
title_short Adaptive Bacteria Colony Picking in Unstructured Environments Using Intensity Histogram and Unascertained LS-SVM Classifier
title_sort adaptive bacteria colony picking in unstructured environments using intensity histogram and unascertained ls svm classifier
url http://dx.doi.org/10.1155/2014/928395
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AT xinli adaptivebacteriacolonypickinginunstructuredenvironmentsusingintensityhistogramandunascertainedlssvmclassifier
AT huiyuzhou adaptivebacteriacolonypickinginunstructuredenvironmentsusingintensityhistogramandunascertainedlssvmclassifier