Chinese cabbage orphan gene BR3 confers bolting resistance to Arabidopsis through the gibberellin pathway
Premature bolting reduces the yield and quality of Chinese cabbage, making bolting resistance gene identification crucial for breeding superior and stable varieties. In this study, we identified an orphan gene BOLTING RESISTANCE 3 (BR3) that positively regulates bolting resistance in Arabidopsis tha...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1518962/full |
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| author | Yuting Zhang Mingliang Jiang Shurui Sun Zongxiang Zhan Xiaonan Li Zhongyun Piao |
| author_facet | Yuting Zhang Mingliang Jiang Shurui Sun Zongxiang Zhan Xiaonan Li Zhongyun Piao |
| author_sort | Yuting Zhang |
| collection | DOAJ |
| description | Premature bolting reduces the yield and quality of Chinese cabbage, making bolting resistance gene identification crucial for breeding superior and stable varieties. In this study, we identified an orphan gene BOLTING RESISTANCE 3 (BR3) that positively regulates bolting resistance in Arabidopsis thaliana. The expression of BR3 was developmentally regulated and occurred during the seedling and flowering stages. The BR3 protein was localized to both the plasma membrane and nucleus. Arabidopsis BR3 overexpressing (BR3OE) plants exhibited delayed bolting and flowering times, an increased number of rosette leaves, reduced plant height, and fewer siliques under long-day (LD) conditions. Key flowering genes were significantly downregulated in BR3OE plants. BR3OE plants similarly exhibited delayed bolting and flowering times, and an increased number of rosette leaves under short-day (SD) conditions. BR3OE plants showed no significant phenotypic differences after vernalization treatment. BR3OE and WT plants exhibited early flowering after GA3 treatment, and bolting and flowering time remained delayed in BR3OE plants compared with WT plants. Key DELLA genes BrRGA1 and BrRGL3 exhibited a co-expression pattern consistent with BR3 gene in Chinese cabbage, which suggested that BrRGA1 and BrRGL3 genes may directly or indirectly regulated by BR3 gene. BR3 gene increased bolting resistance perhaps by upregulating the expression of DELLA genes in the GA pathway. This study provides new theoretical insights for addressing premature bolting in Chinese cabbage and offers novel approaches for breeding bolting-resistant varieties. |
| format | Article |
| id | doaj-art-caa837ca860846f1b455921a16ef3a91 |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj-art-caa837ca860846f1b455921a16ef3a912025-01-20T07:19:57ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-01-011510.3389/fpls.2024.15189621518962Chinese cabbage orphan gene BR3 confers bolting resistance to Arabidopsis through the gibberellin pathwayYuting Zhang0Mingliang Jiang1Shurui Sun2Zongxiang Zhan3Xiaonan Li4Zhongyun Piao5Molecular Biology of Vegetable Laboratory, College of Horticulture, Shenyang Agricultural University, Shenyang, ChinaSchool of Agriculture, Jilin Agricultural Science and Technology University, Jilin, ChinaMolecular Biology of Vegetable Laboratory, College of Horticulture, Shenyang Agricultural University, Shenyang, ChinaMolecular Biology of Vegetable Laboratory, College of Horticulture, Shenyang Agricultural University, Shenyang, ChinaMolecular Biology of Vegetable Laboratory, College of Horticulture, Shenyang Agricultural University, Shenyang, ChinaMolecular Biology of Vegetable Laboratory, College of Horticulture, Shenyang Agricultural University, Shenyang, ChinaPremature bolting reduces the yield and quality of Chinese cabbage, making bolting resistance gene identification crucial for breeding superior and stable varieties. In this study, we identified an orphan gene BOLTING RESISTANCE 3 (BR3) that positively regulates bolting resistance in Arabidopsis thaliana. The expression of BR3 was developmentally regulated and occurred during the seedling and flowering stages. The BR3 protein was localized to both the plasma membrane and nucleus. Arabidopsis BR3 overexpressing (BR3OE) plants exhibited delayed bolting and flowering times, an increased number of rosette leaves, reduced plant height, and fewer siliques under long-day (LD) conditions. Key flowering genes were significantly downregulated in BR3OE plants. BR3OE plants similarly exhibited delayed bolting and flowering times, and an increased number of rosette leaves under short-day (SD) conditions. BR3OE plants showed no significant phenotypic differences after vernalization treatment. BR3OE and WT plants exhibited early flowering after GA3 treatment, and bolting and flowering time remained delayed in BR3OE plants compared with WT plants. Key DELLA genes BrRGA1 and BrRGL3 exhibited a co-expression pattern consistent with BR3 gene in Chinese cabbage, which suggested that BrRGA1 and BrRGL3 genes may directly or indirectly regulated by BR3 gene. BR3 gene increased bolting resistance perhaps by upregulating the expression of DELLA genes in the GA pathway. This study provides new theoretical insights for addressing premature bolting in Chinese cabbage and offers novel approaches for breeding bolting-resistant varieties.https://www.frontiersin.org/articles/10.3389/fpls.2024.1518962/fullChinese cabbageorphan geneBR3bolting resistanceArabidopsisGA pathway |
| spellingShingle | Yuting Zhang Mingliang Jiang Shurui Sun Zongxiang Zhan Xiaonan Li Zhongyun Piao Chinese cabbage orphan gene BR3 confers bolting resistance to Arabidopsis through the gibberellin pathway Frontiers in Plant Science Chinese cabbage orphan gene BR3 bolting resistance Arabidopsis GA pathway |
| title | Chinese cabbage orphan gene BR3 confers bolting resistance to Arabidopsis through the gibberellin pathway |
| title_full | Chinese cabbage orphan gene BR3 confers bolting resistance to Arabidopsis through the gibberellin pathway |
| title_fullStr | Chinese cabbage orphan gene BR3 confers bolting resistance to Arabidopsis through the gibberellin pathway |
| title_full_unstemmed | Chinese cabbage orphan gene BR3 confers bolting resistance to Arabidopsis through the gibberellin pathway |
| title_short | Chinese cabbage orphan gene BR3 confers bolting resistance to Arabidopsis through the gibberellin pathway |
| title_sort | chinese cabbage orphan gene br3 confers bolting resistance to arabidopsis through the gibberellin pathway |
| topic | Chinese cabbage orphan gene BR3 bolting resistance Arabidopsis GA pathway |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1518962/full |
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