Measurement of Telomeric DNA Content in Human Tissues
Telomeres, nucleoprotein complexes at the ends of eukaryotic chromosomes, are 10–12 kbp in length in somatic cells, but as small as 1–2 kbp in rapidly growing cancer cells. Southern blot analysis is currently the standard method for the measurement of telomere length. However, accurate determination...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1997-09-01
|
| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/97233st05 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850152492391727104 |
|---|---|
| author | Jennifer E. Bryant Kent G. Hutchings Robert K. Moyzis Jeffrey K. Griffith |
| author_facet | Jennifer E. Bryant Kent G. Hutchings Robert K. Moyzis Jeffrey K. Griffith |
| author_sort | Jennifer E. Bryant |
| collection | DOAJ |
| description | Telomeres, nucleoprotein complexes at the ends of eukaryotic chromosomes, are 10–12 kbp in length in somatic cells, but as small as 1–2 kbp in rapidly growing cancer cells. Southern blot analysis is currently the standard method for the measurement of telomere length. However, accurate determinations are not possible when DNA is broken or scant. To avoid these problems, a slot blot assay that quantitates the relative content, instead of length, of telomere DNA was developed. The relative contents of telomere DNA determined by this slot blot assay were directly proportional to the relative lengths of telomere DNA determined in parallel by Southern blot analysis. Relative telomere DNA content could be measured in samples containing as little as 15 ng of total DNA. Relative telomere DNA content, but not length, also was unaffected by breakage of DNA into fragments 1 kbp or less in length. |
| format | Article |
| id | doaj-art-caa4d52fd6ed49798d7796c37ef3192b |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 1997-09-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-caa4d52fd6ed49798d7796c37ef3192b2025-08-20T02:25:58ZengTaylor & Francis GroupBioTechniques0736-62051940-98181997-09-0123347648410.2144/97233st05Measurement of Telomeric DNA Content in Human TissuesJennifer E. Bryant0Kent G. Hutchings1Robert K. Moyzis2Jeffrey K. Griffith31University of New Mexico, School of Medicine, Albuquerque1University of New Mexico, School of Medicine, Albuquerque1University of New Mexico, School of Medicine, Albuquerque1University of New Mexico, School of Medicine, AlbuquerqueTelomeres, nucleoprotein complexes at the ends of eukaryotic chromosomes, are 10–12 kbp in length in somatic cells, but as small as 1–2 kbp in rapidly growing cancer cells. Southern blot analysis is currently the standard method for the measurement of telomere length. However, accurate determinations are not possible when DNA is broken or scant. To avoid these problems, a slot blot assay that quantitates the relative content, instead of length, of telomere DNA was developed. The relative contents of telomere DNA determined by this slot blot assay were directly proportional to the relative lengths of telomere DNA determined in parallel by Southern blot analysis. Relative telomere DNA content could be measured in samples containing as little as 15 ng of total DNA. Relative telomere DNA content, but not length, also was unaffected by breakage of DNA into fragments 1 kbp or less in length.https://www.future-science.com/doi/10.2144/97233st05 |
| spellingShingle | Jennifer E. Bryant Kent G. Hutchings Robert K. Moyzis Jeffrey K. Griffith Measurement of Telomeric DNA Content in Human Tissues BioTechniques |
| title | Measurement of Telomeric DNA Content in Human Tissues |
| title_full | Measurement of Telomeric DNA Content in Human Tissues |
| title_fullStr | Measurement of Telomeric DNA Content in Human Tissues |
| title_full_unstemmed | Measurement of Telomeric DNA Content in Human Tissues |
| title_short | Measurement of Telomeric DNA Content in Human Tissues |
| title_sort | measurement of telomeric dna content in human tissues |
| url | https://www.future-science.com/doi/10.2144/97233st05 |
| work_keys_str_mv | AT jenniferebryant measurementoftelomericdnacontentinhumantissues AT kentghutchings measurementoftelomericdnacontentinhumantissues AT robertkmoyzis measurementoftelomericdnacontentinhumantissues AT jeffreykgriffith measurementoftelomericdnacontentinhumantissues |