Nano-selenium mitigates arsenate toxicity in soybean roots by modulating phenylalanine and salicylic acid pathways

Abstract Background Soybean (Glycine max L. Merrill), a vital source of edible oil and protein, ranks seventh in global agricultural production, yet its productivity is significantly hindered by potential toxic metal/liods (PTM) stress. Arsenic (As), a highly toxic soil contaminant, poses substantia...

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Main Authors: Muhammad Zeeshan, Aamir Hamid Khan, Abdul Salam, Yuxin Hu, Anas Iqbal, Ruiquan Hou, Abdul Wakeel Umar, Feibo Wu, Xiaoyuan Chen, Zhixiang Zhang
Format: Article
Language:English
Published: BMC 2025-05-01
Series:BMC Plant Biology
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Online Access:https://doi.org/10.1186/s12870-025-06726-0
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author Muhammad Zeeshan
Aamir Hamid Khan
Abdul Salam
Yuxin Hu
Anas Iqbal
Ruiquan Hou
Abdul Wakeel Umar
Feibo Wu
Xiaoyuan Chen
Zhixiang Zhang
author_facet Muhammad Zeeshan
Aamir Hamid Khan
Abdul Salam
Yuxin Hu
Anas Iqbal
Ruiquan Hou
Abdul Wakeel Umar
Feibo Wu
Xiaoyuan Chen
Zhixiang Zhang
author_sort Muhammad Zeeshan
collection DOAJ
description Abstract Background Soybean (Glycine max L. Merrill), a vital source of edible oil and protein, ranks seventh in global agricultural production, yet its productivity is significantly hindered by potential toxic metal/liods (PTM) stress. Arsenic (As), a highly toxic soil contaminant, poses substantial risks to both plants and humans, even at trace concentrations, particularly in China. Results This research endeavor delves into the combined effect of arsenate (AsV), a common form of As in soil, and nano-selenium (nSe), on the transcriptional regulation of key genes and the modulation of signaling and metabolic cascades in young soybean seedlings. Our findings indicate that nSe mitigates AsV toxicity by modulating hormonal signaling cascades, particularly the phenylalanine and salicylic acid pathways, thereby augmenting antioxidant defenses and mitigating the damaging effects of reactive oxygen species (ROS) on soybean roots. Conclusion This study offers valuable insights into the molecular mechanisms underlying metalloid tolerance in soybean, opening avenues for the development of strategies to bolster As resistance in contaminated soils. Nevertheless, further investigation is imperative to elucidate the intricate interplay of hormonal signaling in soybean roots during nSe supplementation under As stress conditions.
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publishDate 2025-05-01
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spelling doaj-art-caa330465f3a4d64b4f9c789c0c08d212025-08-20T02:03:28ZengBMCBMC Plant Biology1471-22292025-05-0125111710.1186/s12870-025-06726-0Nano-selenium mitigates arsenate toxicity in soybean roots by modulating phenylalanine and salicylic acid pathwaysMuhammad Zeeshan0Aamir Hamid Khan1Abdul Salam2Yuxin Hu3Anas Iqbal4Ruiquan Hou5Abdul Wakeel Umar6Feibo Wu7Xiaoyuan Chen8Zhixiang Zhang9State Key Laboratory of Green Pesticide, South China Agricultural UniversityDepartment of Biogeography, Paleoecology and Nature Conservation, Faculty of Biology and Environmental Protection, University of ŁódźState Key Laboratory of Green Pesticide, South China Agricultural UniversityCollege of Pastoral Agriculture Science and Technology, Lanzhou UniversityGuangzhou Key Laboratory for Science and Technology of Fragrant RiceState Key Laboratory of Green Pesticide, South China Agricultural UniversityBNU-HKUST Laboratory of Green Innovation, Advanced Institute of Natural Sciences, Beijing Normal University at Zhuhai (BNUZ)Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang UniversityYingdong College of Biology and Agriculture, Shaoguan UniversityState Key Laboratory of Green Pesticide, South China Agricultural UniversityAbstract Background Soybean (Glycine max L. Merrill), a vital source of edible oil and protein, ranks seventh in global agricultural production, yet its productivity is significantly hindered by potential toxic metal/liods (PTM) stress. Arsenic (As), a highly toxic soil contaminant, poses substantial risks to both plants and humans, even at trace concentrations, particularly in China. Results This research endeavor delves into the combined effect of arsenate (AsV), a common form of As in soil, and nano-selenium (nSe), on the transcriptional regulation of key genes and the modulation of signaling and metabolic cascades in young soybean seedlings. Our findings indicate that nSe mitigates AsV toxicity by modulating hormonal signaling cascades, particularly the phenylalanine and salicylic acid pathways, thereby augmenting antioxidant defenses and mitigating the damaging effects of reactive oxygen species (ROS) on soybean roots. Conclusion This study offers valuable insights into the molecular mechanisms underlying metalloid tolerance in soybean, opening avenues for the development of strategies to bolster As resistance in contaminated soils. Nevertheless, further investigation is imperative to elucidate the intricate interplay of hormonal signaling in soybean roots during nSe supplementation under As stress conditions.https://doi.org/10.1186/s12870-025-06726-0Glycine maxSelenium nanoparticlesArsenic stressHormonal regulationTranscriptomic
spellingShingle Muhammad Zeeshan
Aamir Hamid Khan
Abdul Salam
Yuxin Hu
Anas Iqbal
Ruiquan Hou
Abdul Wakeel Umar
Feibo Wu
Xiaoyuan Chen
Zhixiang Zhang
Nano-selenium mitigates arsenate toxicity in soybean roots by modulating phenylalanine and salicylic acid pathways
BMC Plant Biology
Glycine max
Selenium nanoparticles
Arsenic stress
Hormonal regulation
Transcriptomic
title Nano-selenium mitigates arsenate toxicity in soybean roots by modulating phenylalanine and salicylic acid pathways
title_full Nano-selenium mitigates arsenate toxicity in soybean roots by modulating phenylalanine and salicylic acid pathways
title_fullStr Nano-selenium mitigates arsenate toxicity in soybean roots by modulating phenylalanine and salicylic acid pathways
title_full_unstemmed Nano-selenium mitigates arsenate toxicity in soybean roots by modulating phenylalanine and salicylic acid pathways
title_short Nano-selenium mitigates arsenate toxicity in soybean roots by modulating phenylalanine and salicylic acid pathways
title_sort nano selenium mitigates arsenate toxicity in soybean roots by modulating phenylalanine and salicylic acid pathways
topic Glycine max
Selenium nanoparticles
Arsenic stress
Hormonal regulation
Transcriptomic
url https://doi.org/10.1186/s12870-025-06726-0
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