Metabolite acetyl-L-carnitine participates in Bifidobacterium animalis F1-7 to ameliorate atherosclerotic inflammation by downregulating theTLR4/NF-κB pathway
This study aimed to explore the effect of Bif idobacterium animalis F1-7 on the improvement of atherosclerotic inflammation. Arteriosclerosis model ApoE-/- mice were orally administered with B. animalis F1-7 for 12 weeks. The probiotic intervention reduced the plaque areas in aorta and the accumulat...
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Tsinghua University Press
2024-03-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2022.9250069 |
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| author | Xi Liang Zhe Zhang Xiaoying Tian Qingyu Cui Haiyan Lu Maozhen Zhao Tongjie Liu Huaxi Yi Pimin Gong Lanwei Zhang |
| author_facet | Xi Liang Zhe Zhang Xiaoying Tian Qingyu Cui Haiyan Lu Maozhen Zhao Tongjie Liu Huaxi Yi Pimin Gong Lanwei Zhang |
| author_sort | Xi Liang |
| collection | DOAJ |
| description | This study aimed to explore the effect of Bif idobacterium animalis F1-7 on the improvement of atherosclerotic inflammation. Arteriosclerosis model ApoE-/- mice were orally administered with B. animalis F1-7 for 12 weeks. The probiotic intervention reduced the plaque areas in aorta and the accumulation of macrophages, and downregulated the expression of toll-like receptor 4 (TLR4)/nuclear factor κB (NF-κB) pathway to reduce the levels of inf lammatory factors. The widely-targeted metabolomics analysis showed that acetyl-L-carnitine (ALC) in the intestine of atherosclerotic mice was signif icantly increased after B. animalis F1-7 intervention. Correlation analysis proved that ALC was associated with atherosclerotic inf lammatory response. By using oxidized low density lipoprotein induced macrophage foam cells, we further verif ied that ALC could reduce lipid accumulation and inf lammatory response in foam cells by downregulating the TLR4/NF-κB pathway. Finally, our results revealed that B. animalis F1-7 upregulated the metabolite ALC to downregulate the inf lammatory responses, leading to the reduction of plaque accumulation of atherosclerosis. |
| format | Article |
| id | doaj-art-caa301607deb48c698fb0bb8ba721b78 |
| institution | DOAJ |
| issn | 2213-4530 |
| language | English |
| publishDate | 2024-03-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-caa301607deb48c698fb0bb8ba721b782025-08-20T03:24:04ZengTsinghua University PressFood Science and Human Wellness2213-45302024-03-0113281382210.26599/FSHW.2022.9250069Metabolite acetyl-L-carnitine participates in Bifidobacterium animalis F1-7 to ameliorate atherosclerotic inflammation by downregulating theTLR4/NF-κB pathwayXi Liang0Zhe Zhang1Xiaoying Tian2Qingyu Cui3Haiyan Lu4Maozhen Zhao5Tongjie Liu6Huaxi Yi7Pimin Gong8Lanwei Zhang9Department of Nutrition and Food Hygiene, School of public health, Qingdao University, Qingdao 266071, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaThis study aimed to explore the effect of Bif idobacterium animalis F1-7 on the improvement of atherosclerotic inflammation. Arteriosclerosis model ApoE-/- mice were orally administered with B. animalis F1-7 for 12 weeks. The probiotic intervention reduced the plaque areas in aorta and the accumulation of macrophages, and downregulated the expression of toll-like receptor 4 (TLR4)/nuclear factor κB (NF-κB) pathway to reduce the levels of inf lammatory factors. The widely-targeted metabolomics analysis showed that acetyl-L-carnitine (ALC) in the intestine of atherosclerotic mice was signif icantly increased after B. animalis F1-7 intervention. Correlation analysis proved that ALC was associated with atherosclerotic inf lammatory response. By using oxidized low density lipoprotein induced macrophage foam cells, we further verif ied that ALC could reduce lipid accumulation and inf lammatory response in foam cells by downregulating the TLR4/NF-κB pathway. Finally, our results revealed that B. animalis F1-7 upregulated the metabolite ALC to downregulate the inf lammatory responses, leading to the reduction of plaque accumulation of atherosclerosis.https://www.sciopen.com/article/10.26599/FSHW.2022.9250069bifidobacterium animalis f1-7atherosclerosisinflammationmetabonomicsacetyl-l-carnitine |
| spellingShingle | Xi Liang Zhe Zhang Xiaoying Tian Qingyu Cui Haiyan Lu Maozhen Zhao Tongjie Liu Huaxi Yi Pimin Gong Lanwei Zhang Metabolite acetyl-L-carnitine participates in Bifidobacterium animalis F1-7 to ameliorate atherosclerotic inflammation by downregulating theTLR4/NF-κB pathway Food Science and Human Wellness bifidobacterium animalis f1-7 atherosclerosis inflammation metabonomics acetyl-l-carnitine |
| title | Metabolite acetyl-L-carnitine participates in Bifidobacterium animalis F1-7 to ameliorate atherosclerotic inflammation by downregulating theTLR4/NF-κB pathway |
| title_full | Metabolite acetyl-L-carnitine participates in Bifidobacterium animalis F1-7 to ameliorate atherosclerotic inflammation by downregulating theTLR4/NF-κB pathway |
| title_fullStr | Metabolite acetyl-L-carnitine participates in Bifidobacterium animalis F1-7 to ameliorate atherosclerotic inflammation by downregulating theTLR4/NF-κB pathway |
| title_full_unstemmed | Metabolite acetyl-L-carnitine participates in Bifidobacterium animalis F1-7 to ameliorate atherosclerotic inflammation by downregulating theTLR4/NF-κB pathway |
| title_short | Metabolite acetyl-L-carnitine participates in Bifidobacterium animalis F1-7 to ameliorate atherosclerotic inflammation by downregulating theTLR4/NF-κB pathway |
| title_sort | metabolite acetyl l carnitine participates in bifidobacterium animalis f1 7 to ameliorate atherosclerotic inflammation by downregulating thetlr4 nf κb pathway |
| topic | bifidobacterium animalis f1-7 atherosclerosis inflammation metabonomics acetyl-l-carnitine |
| url | https://www.sciopen.com/article/10.26599/FSHW.2022.9250069 |
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