First Report of Agrobacterium tumefaciens mediated genetic transformation of aquatic Rice paddy herb (Limnophila aromatica)
The study presents first report of Agrobacterium mediated genetic transformation in Rice paddy herb (Linmophila aromatica). A. tumefaciens strain C58C1 harboring pBin19 Plasmid containing β-glucuronidase (GUS) and neomycin phosphotransferase II (nptII) gene, under the control of 35S promoter and NO...
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| Format: | Article |
| Language: | English |
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Hasan Eleroğlu
2016-08-01
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| Series: | Turkish Journal of Agriculture: Food Science and Technology |
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| Online Access: | http://www.agrifoodscience.com/index.php/TURJAF/article/view/747 |
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| author | Allah Bakhsh Muhammad Aasim Muhammad Abu Bakar Zia Muhammet Doğan Gökhan Sadı Mehmet Karataş Khalid Mahmood Khawar |
| author_facet | Allah Bakhsh Muhammad Aasim Muhammad Abu Bakar Zia Muhammet Doğan Gökhan Sadı Mehmet Karataş Khalid Mahmood Khawar |
| author_sort | Allah Bakhsh |
| collection | DOAJ |
| description | The study presents first report of Agrobacterium mediated genetic transformation in Rice paddy herb (Linmophila aromatica). A. tumefaciens strain C58C1 harboring pBin19 Plasmid containing β-glucuronidase (GUS) and neomycin phosphotransferase II (nptII) gene, under the control of 35S promoter and NOS terminator was used. Shoot tip explants were inoculated for 30 min followed by co-cultivation for 72 h and selected on agar semi solidified MS medium containing 100 mg/l Kanamycin and 1.0 mg/l BA; whereas total number of 78 putative transgenic shoots were obtained. The shoots were rooted on MS medium containing 1.0 mg/l IBA and 100 mg/l Kanamycin where 43 plants survived and rooted. Expression of GUS gene in the putative transgenics was confirmed by histochemical GUS assay. Visible localised gus expression was noted in a few cells and callus tissues of 4 plantlets that were photographed using compound light microscope. |
| format | Article |
| id | doaj-art-caa2c7be265d4a9f9d3402c87fc4e8ea |
| institution | OA Journals |
| issn | 2148-127X |
| language | English |
| publishDate | 2016-08-01 |
| publisher | Hasan Eleroğlu |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj-art-caa2c7be265d4a9f9d3402c87fc4e8ea2025-08-20T02:19:37ZengHasan EleroğluTurkish Journal of Agriculture: Food Science and Technology2148-127X2016-08-014864264510.24925/turjaf.v4i8.642-645.747356First Report of Agrobacterium tumefaciens mediated genetic transformation of aquatic Rice paddy herb (Limnophila aromatica)Allah Bakhsh0Muhammad Aasim1Muhammad Abu Bakar Zia2Muhammet Doğan3Gökhan Sadı4Mehmet Karataş5Khalid Mahmood Khawar6Department of Agricultural Genetic Engineering, Faculty of Agricultural Sciences and Technology, Niğde University, 51240 NiğdeDepartment of Biology, Kamil Özdağ Faculty of Sciences, Karamanoğlu Mehmetbey University, 70200 KaramanDepartment of Agricultural Genetic Engineering, Faculty of Agricultural Sciences and Technology, Niğde University, 51240 NiğdeDepartment of Biology, Kamil Özdağ Faculty of Sciences, Karamanoğlu Mehmetbey University, 70200 KaramanDepartment of Biology, Kamil Özdağ Faculty of Sciences, Karamanoğlu Mehmetbey University, 70200 KaramanDepartment of Agricultural Genetic Engineering, Faculty of Agricultural Sciences and Technology, Niğde University, 51240 NiğdeDepartment of Field Crops, Faculty of Agriculture, Ankara University, 0611 Dışkapı/AnkaraThe study presents first report of Agrobacterium mediated genetic transformation in Rice paddy herb (Linmophila aromatica). A. tumefaciens strain C58C1 harboring pBin19 Plasmid containing β-glucuronidase (GUS) and neomycin phosphotransferase II (nptII) gene, under the control of 35S promoter and NOS terminator was used. Shoot tip explants were inoculated for 30 min followed by co-cultivation for 72 h and selected on agar semi solidified MS medium containing 100 mg/l Kanamycin and 1.0 mg/l BA; whereas total number of 78 putative transgenic shoots were obtained. The shoots were rooted on MS medium containing 1.0 mg/l IBA and 100 mg/l Kanamycin where 43 plants survived and rooted. Expression of GUS gene in the putative transgenics was confirmed by histochemical GUS assay. Visible localised gus expression was noted in a few cells and callus tissues of 4 plantlets that were photographed using compound light microscope.http://www.agrifoodscience.com/index.php/TURJAF/article/view/747AgrobacteriumGenetic transformationShoot tipGUSnptII |
| spellingShingle | Allah Bakhsh Muhammad Aasim Muhammad Abu Bakar Zia Muhammet Doğan Gökhan Sadı Mehmet Karataş Khalid Mahmood Khawar First Report of Agrobacterium tumefaciens mediated genetic transformation of aquatic Rice paddy herb (Limnophila aromatica) Turkish Journal of Agriculture: Food Science and Technology Agrobacterium Genetic transformation Shoot tip GUS nptII |
| title | First Report of Agrobacterium tumefaciens mediated genetic transformation of aquatic Rice paddy herb (Limnophila aromatica) |
| title_full | First Report of Agrobacterium tumefaciens mediated genetic transformation of aquatic Rice paddy herb (Limnophila aromatica) |
| title_fullStr | First Report of Agrobacterium tumefaciens mediated genetic transformation of aquatic Rice paddy herb (Limnophila aromatica) |
| title_full_unstemmed | First Report of Agrobacterium tumefaciens mediated genetic transformation of aquatic Rice paddy herb (Limnophila aromatica) |
| title_short | First Report of Agrobacterium tumefaciens mediated genetic transformation of aquatic Rice paddy herb (Limnophila aromatica) |
| title_sort | first report of agrobacterium tumefaciens mediated genetic transformation of aquatic rice paddy herb limnophila aromatica |
| topic | Agrobacterium Genetic transformation Shoot tip GUS nptII |
| url | http://www.agrifoodscience.com/index.php/TURJAF/article/view/747 |
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