Molecular epidemiology of Cryptosporidium spp. in North Lebanon
Cryptosporidium spp. are enteroparasites with worldwide distribution that infect the gastrointestinal tract of several vertebrates including humans. Human to human, zoonotic, foodborne and waterborne are reported as the main transmission routes of this parasite. Cryptosporidium spp. have been recog...
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The Journal of Infection in Developing Countries
2018-02-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/10014 |
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| author | Marwan Osman Sadia Benamrouz Karine Guyot Dima El Safadi Hassan Mallat Fouad Dabboussi Monzer Hamze Eric Viscogliosi Gabriela Certad |
| author_facet | Marwan Osman Sadia Benamrouz Karine Guyot Dima El Safadi Hassan Mallat Fouad Dabboussi Monzer Hamze Eric Viscogliosi Gabriela Certad |
| author_sort | Marwan Osman |
| collection | DOAJ |
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Cryptosporidium spp. are enteroparasites with worldwide distribution that infect the gastrointestinal tract of several vertebrates including humans. Human to human, zoonotic, foodborne and waterborne are reported as the main transmission routes of this parasite. Cryptosporidium spp. have been recognized as the predominant cause of waterborne and foodborne outbreaks. However, the epidemiological situation of cryptosporidiosis is not well known in Lebanon, a developing country with a population often affected by intestinal parasitic infections.
This study was devoted to determine the prevalence and the genetic diversity of Cryptosporidium spp. in symptomatic hospitalized patients and in two children populations with different socio-economic level in North-Lebanon, as well as the risk factors associated with cryptosporidiosis.
Fecal samples obtained from these populations were examined microscopically by modified Ziehl-Neelsen staining as well as nested PCR were done for the detection of Cryptosporidium oocysts.
Out of 163 symptomatic hospitalized patients and 249 children, Cryptosporidium was present in 11% and 10.4% respectively according to microscopy examination and/or molecular tests. The genotyping showed the predominance of Cryptosporidium hominis in both populations. Subgenotype analysis of the isolates at the gp60 locus identified three subtypes IdA19, IbA10G2 and IaA18R3 for C. hominis and two subtypes IIaA15G1R1 and IIaA15G2R1 for C. parvum. Moreover, cryptosporidiosis was correlated with having meals outside home and presence of gastrointestinal symptoms especially diarrhea (p <0.05).
This work constitutes the first molecular epidemiology study outlining risk factors associated with cryptosporidiosis in Lebanon. These findings support a need of a control program to prevent the circulation of this parasite.
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| format | Article |
| id | doaj-art-ca9df12dd92c482c847703bd7c9051d7 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2018-02-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-ca9df12dd92c482c847703bd7c9051d72025-08-20T02:16:14ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802018-02-011202.110.3855/jidc.10014Molecular epidemiology of Cryptosporidium spp. in North LebanonMarwan Osman0Sadia Benamrouz1Karine Guyot2Dima El Safadi3Hassan Mallat4Fouad Dabboussi5Monzer Hamze6Eric Viscogliosi7Gabriela Certad8Ecole Doctorale des Sciences et de Technologie, Faculté de Santé Publique, Université Libanaise, Tripoli, LibanUniversité de Lille, CHU de Lille, Biologie et Diversité des Pathogènes Eucaryotes Emergents (BDPEE), Lille, FranceUniversité de Lille, CHU de Lille, Biologie et Diversité des Pathogènes Eucaryotes Emergents (BDPEE), Lille, FranceEcole Doctorale des Sciences et de Technologie, Faculté de Santé Publique, Université Libanaise, Tripoli, LibanEcole Doctorale des Sciences et de Technologie, Faculté de Santé Publique, Université Libanaise, Tripoli, LibanEcole Doctorale des Sciences et de Technologie, Faculté de Santé Publique, Université Libanaise, Tripoli, LibanEcole Doctorale des Sciences et de Technologie, Faculté de Santé Publique, Université Libanaise, Tripoli, LibanUniversité de Lille, CHU de Lille, Biologie et Diversité des Pathogènes Eucaryotes Emergents (BDPEE), Lille, FranceUniversité de Lille, CHU de Lille, Biologie et Diversité des Pathogènes Eucaryotes Emergents (BDPEE), Lille, France Cryptosporidium spp. are enteroparasites with worldwide distribution that infect the gastrointestinal tract of several vertebrates including humans. Human to human, zoonotic, foodborne and waterborne are reported as the main transmission routes of this parasite. Cryptosporidium spp. have been recognized as the predominant cause of waterborne and foodborne outbreaks. However, the epidemiological situation of cryptosporidiosis is not well known in Lebanon, a developing country with a population often affected by intestinal parasitic infections. This study was devoted to determine the prevalence and the genetic diversity of Cryptosporidium spp. in symptomatic hospitalized patients and in two children populations with different socio-economic level in North-Lebanon, as well as the risk factors associated with cryptosporidiosis. Fecal samples obtained from these populations were examined microscopically by modified Ziehl-Neelsen staining as well as nested PCR were done for the detection of Cryptosporidium oocysts. Out of 163 symptomatic hospitalized patients and 249 children, Cryptosporidium was present in 11% and 10.4% respectively according to microscopy examination and/or molecular tests. The genotyping showed the predominance of Cryptosporidium hominis in both populations. Subgenotype analysis of the isolates at the gp60 locus identified three subtypes IdA19, IbA10G2 and IaA18R3 for C. hominis and two subtypes IIaA15G1R1 and IIaA15G2R1 for C. parvum. Moreover, cryptosporidiosis was correlated with having meals outside home and presence of gastrointestinal symptoms especially diarrhea (p <0.05). This work constitutes the first molecular epidemiology study outlining risk factors associated with cryptosporidiosis in Lebanon. These findings support a need of a control program to prevent the circulation of this parasite. https://jidc.org/index.php/journal/article/view/10014Cryptosporidium spp.Molecular EpidemiologyGenotypingRisk factorsLebanon |
| spellingShingle | Marwan Osman Sadia Benamrouz Karine Guyot Dima El Safadi Hassan Mallat Fouad Dabboussi Monzer Hamze Eric Viscogliosi Gabriela Certad Molecular epidemiology of Cryptosporidium spp. in North Lebanon Journal of Infection in Developing Countries Cryptosporidium spp. Molecular Epidemiology Genotyping Risk factors Lebanon |
| title | Molecular epidemiology of Cryptosporidium spp. in North Lebanon |
| title_full | Molecular epidemiology of Cryptosporidium spp. in North Lebanon |
| title_fullStr | Molecular epidemiology of Cryptosporidium spp. in North Lebanon |
| title_full_unstemmed | Molecular epidemiology of Cryptosporidium spp. in North Lebanon |
| title_short | Molecular epidemiology of Cryptosporidium spp. in North Lebanon |
| title_sort | molecular epidemiology of cryptosporidium spp in north lebanon |
| topic | Cryptosporidium spp. Molecular Epidemiology Genotyping Risk factors Lebanon |
| url | https://jidc.org/index.php/journal/article/view/10014 |
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