Frog‐Derived Neuroregenerative‐Peptide‐Reinforced Bioactive Hydrogels with Injectability, Thermosensitivity, and Enhanced Neurotrophic Function for Repairing Central Nervous Injuries
The clinical treatment of central nervous system (CNS) injuries presents significant challenges due to the inflammatory microenvironment (IME) induced by CNS injury, which inhibits spontaneous neuronal regeneration. Biomimetic biomaterial‐based IMEs for CNS repair, which facilitate the reconstructio...
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Wiley-VCH
2025-07-01
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| Series: | Small Structures |
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| Online Access: | https://doi.org/10.1002/sstr.202400572 |
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| author | Zhengang Sun Zhenjuan Sun Chenxi Du Hongyu Chen Xingchao Zhang Yuanyuan Liu Fang Dong Xuexiao Ma Gang Wei Haiyuan Yue |
| author_facet | Zhengang Sun Zhenjuan Sun Chenxi Du Hongyu Chen Xingchao Zhang Yuanyuan Liu Fang Dong Xuexiao Ma Gang Wei Haiyuan Yue |
| author_sort | Zhengang Sun |
| collection | DOAJ |
| description | The clinical treatment of central nervous system (CNS) injuries presents significant challenges due to the inflammatory microenvironment (IME) induced by CNS injury, which inhibits spontaneous neuronal regeneration. Biomimetic biomaterial‐based IMEs for CNS repair, which facilitate the reconstruction of neural regeneration circuits, show promise. In this study, the development of novel bioactive peptide nanofibers (PNFs)/chitosan (CS)/VD11 (VDELWPPWLPC) hydrogels (named as PCV) is reported, created by reinforcing composite PNFs/CS with a frog‐derived neuroregenerative peptide (VD11). The created hydrogels exhibit a 3D porous structure, high thermosensitivity, good injectability, and enhanced neurotrophic properties, making them promising candidates for CNS repair. The in vitro tests indicate that PCV hydrogels can promote the proliferation, migration, and differentiation of stem cells into neurons, as well as guide axonal growth. Additionally, they help mitigate inflammatory responses by reducing macrophage activation and astrocyte proliferation, while promoting neovascularization. The in vivo animal experimentsdemonstrate that PCV hydrogels enhance blood supply to the damaged area and promote the migration and colonization of endogenous neural stem cells to support axonal growth. Furthermore, PCV hydrogels reduce immune responses and limit excessive astrocyte proliferation, significantly improving motor function recovery in rats with spinal cord injuries. In these findings, it is suggested that these hydrogels can provide a promising strategy for treating CNS injuries by regulating IME. |
| format | Article |
| id | doaj-art-ca96bb136fb040988f6120efe27e8e26 |
| institution | Kabale University |
| issn | 2688-4062 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Small Structures |
| spelling | doaj-art-ca96bb136fb040988f6120efe27e8e262025-08-20T03:28:40ZengWiley-VCHSmall Structures2688-40622025-07-0167n/an/a10.1002/sstr.202400572Frog‐Derived Neuroregenerative‐Peptide‐Reinforced Bioactive Hydrogels with Injectability, Thermosensitivity, and Enhanced Neurotrophic Function for Repairing Central Nervous InjuriesZhengang Sun0Zhenjuan Sun1Chenxi Du2Hongyu Chen3Xingchao Zhang4Yuanyuan Liu5Fang Dong6Xuexiao Ma7Gang Wei8Haiyuan Yue9Department of Spinal Surgery Qingdao Huangdao Central Hospital Qingdao 266555 P. R. ChinaInformation statistics section The Sixth People's Hospital of Qingdao Qingdao 266000 P. R. ChinaCollege of Chemistry and Chemical Engineering Qingdao University Qingdao 266071 P. R. ChinaDepartment of Spinal Surgery Qingdao Huangdao Central Hospital Qingdao 266555 P. R. ChinaDepartment of Spinal Surgery Qingdao Huangdao Central Hospital Qingdao 266555 P. R. ChinaDepartment of Spinal Surgery Qingdao Huangdao Central Hospital Qingdao 266555 P. R. ChinaMacrovascular surgery Gansu Provincial Hospital Lanzhou Gansu 730000 ChinaDepartment of Spinal Surgery The Affiliated Hospital of Qingdao University Qingdao 266071 P. R. ChinaCollege of Chemistry and Chemical Engineering Qingdao University Qingdao 266071 P. R. ChinaDepartment of Orthopaedics Lanzhou University Second Hospital Lanzhou Gansu 730030 P. R. ChinaThe clinical treatment of central nervous system (CNS) injuries presents significant challenges due to the inflammatory microenvironment (IME) induced by CNS injury, which inhibits spontaneous neuronal regeneration. Biomimetic biomaterial‐based IMEs for CNS repair, which facilitate the reconstruction of neural regeneration circuits, show promise. In this study, the development of novel bioactive peptide nanofibers (PNFs)/chitosan (CS)/VD11 (VDELWPPWLPC) hydrogels (named as PCV) is reported, created by reinforcing composite PNFs/CS with a frog‐derived neuroregenerative peptide (VD11). The created hydrogels exhibit a 3D porous structure, high thermosensitivity, good injectability, and enhanced neurotrophic properties, making them promising candidates for CNS repair. The in vitro tests indicate that PCV hydrogels can promote the proliferation, migration, and differentiation of stem cells into neurons, as well as guide axonal growth. Additionally, they help mitigate inflammatory responses by reducing macrophage activation and astrocyte proliferation, while promoting neovascularization. The in vivo animal experimentsdemonstrate that PCV hydrogels enhance blood supply to the damaged area and promote the migration and colonization of endogenous neural stem cells to support axonal growth. Furthermore, PCV hydrogels reduce immune responses and limit excessive astrocyte proliferation, significantly improving motor function recovery in rats with spinal cord injuries. In these findings, it is suggested that these hydrogels can provide a promising strategy for treating CNS injuries by regulating IME.https://doi.org/10.1002/sstr.202400572bioactive hydrogelsbiomimetic peptidescentral nervous injuriesneuroregenerationspeptide nanofibers |
| spellingShingle | Zhengang Sun Zhenjuan Sun Chenxi Du Hongyu Chen Xingchao Zhang Yuanyuan Liu Fang Dong Xuexiao Ma Gang Wei Haiyuan Yue Frog‐Derived Neuroregenerative‐Peptide‐Reinforced Bioactive Hydrogels with Injectability, Thermosensitivity, and Enhanced Neurotrophic Function for Repairing Central Nervous Injuries Small Structures bioactive hydrogels biomimetic peptides central nervous injuries neuroregenerations peptide nanofibers |
| title | Frog‐Derived Neuroregenerative‐Peptide‐Reinforced Bioactive Hydrogels with Injectability, Thermosensitivity, and Enhanced Neurotrophic Function for Repairing Central Nervous Injuries |
| title_full | Frog‐Derived Neuroregenerative‐Peptide‐Reinforced Bioactive Hydrogels with Injectability, Thermosensitivity, and Enhanced Neurotrophic Function for Repairing Central Nervous Injuries |
| title_fullStr | Frog‐Derived Neuroregenerative‐Peptide‐Reinforced Bioactive Hydrogels with Injectability, Thermosensitivity, and Enhanced Neurotrophic Function for Repairing Central Nervous Injuries |
| title_full_unstemmed | Frog‐Derived Neuroregenerative‐Peptide‐Reinforced Bioactive Hydrogels with Injectability, Thermosensitivity, and Enhanced Neurotrophic Function for Repairing Central Nervous Injuries |
| title_short | Frog‐Derived Neuroregenerative‐Peptide‐Reinforced Bioactive Hydrogels with Injectability, Thermosensitivity, and Enhanced Neurotrophic Function for Repairing Central Nervous Injuries |
| title_sort | frog derived neuroregenerative peptide reinforced bioactive hydrogels with injectability thermosensitivity and enhanced neurotrophic function for repairing central nervous injuries |
| topic | bioactive hydrogels biomimetic peptides central nervous injuries neuroregenerations peptide nanofibers |
| url | https://doi.org/10.1002/sstr.202400572 |
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