Prevalence and Molecular Characterization of Porcine Parvovirus 2 in Southwest China During 2020–2023
Porcine parvovirus (PPV) is a non-enveloped, single-stranded linear DNA virus that induces reproductive disorders in sows, particularly abortions in primiparous sows. This study investigated the prevalence of PPV in the southwestern region and conducted molecular characterization of PPV strains. An...
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MDPI AG
2025-01-01
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| author | Hongyu Chen Yi Qing Lei Xu Ling Zhu Wenqi Yin Shuwei Li Shengyao Kuang Yuancheng Zhou Zhiwen Xu |
| author_facet | Hongyu Chen Yi Qing Lei Xu Ling Zhu Wenqi Yin Shuwei Li Shengyao Kuang Yuancheng Zhou Zhiwen Xu |
| author_sort | Hongyu Chen |
| collection | DOAJ |
| description | Porcine parvovirus (PPV) is a non-enveloped, single-stranded linear DNA virus that induces reproductive disorders in sows, particularly abortions in primiparous sows. This study investigated the prevalence of PPV in the southwestern region and conducted molecular characterization of PPV strains. An epidemiological survey was conducted on 1534 aborted fetuses from the southwestern region between 2020 and 2023, revealing an abortion rate of 3.00% due to PPV2, with the highest rate of 3.77% in Sichuan. Additionally, 2973 blood samples from sows were tested using ELISA, showing a PPV2 antibody positivity rate of 73.03% to 90%. Through shotgun metagenomics, PPV2 SC2020 was identified in aborted fetal samples from a pig farm in Pengzhou, Sichuan. PCR sequencing analysis yielded seven PPV2 genomic sequences, and the phylogenetic analysis of eight PPV2 strains with thirty reference strains showed distinct evolutionary branches. The virus was successfully isolated from PPV2-positive samples, and the phylogenetic analysis of PPV2 SC2020 revealed ORF1 gene homology of 94.9% to 99.3% and the ORF2 gene homology of 93.1% to 98.0%, with 34 reference strains. Homologous recombination analysis indicated that SC2020 is a recombinant strain of HeB03 and S1. |
| format | Article |
| id | doaj-art-ca8d615ebe5e44a29ddf017cc3868428 |
| institution | DOAJ |
| issn | 2306-7381 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj-art-ca8d615ebe5e44a29ddf017cc38684282025-08-20T03:12:10ZengMDPI AGVeterinary Sciences2306-73812025-01-011229910.3390/vetsci12020099Prevalence and Molecular Characterization of Porcine Parvovirus 2 in Southwest China During 2020–2023Hongyu Chen0Yi Qing1Lei Xu2Ling Zhu3Wenqi Yin4Shuwei Li5Shengyao Kuang6Yuancheng Zhou7Zhiwen Xu8Key Laboratory of Animal Diseases and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaChengdu Livestock and Poultry Genetic Resources Protection Center, Chengdu 610081, ChinaCollege of Animal Husbandry and Veterinary Medicine, Chengdu Agricultural College, Chengdu 610000, ChinaKey Laboratory of Animal Diseases and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaLivestock and Poultry Biological Products Key Laboratory of Sichuan Province, Sichuan Animal Science Academy, Chengdu 610000, ChinaLivestock and Poultry Biological Products Key Laboratory of Sichuan Province, Sichuan Animal Science Academy, Chengdu 610000, ChinaLivestock and Poultry Biological Products Key Laboratory of Sichuan Province, Sichuan Animal Science Academy, Chengdu 610000, ChinaLivestock and Poultry Biological Products Key Laboratory of Sichuan Province, Sichuan Animal Science Academy, Chengdu 610000, ChinaKey Laboratory of Animal Diseases and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaPorcine parvovirus (PPV) is a non-enveloped, single-stranded linear DNA virus that induces reproductive disorders in sows, particularly abortions in primiparous sows. This study investigated the prevalence of PPV in the southwestern region and conducted molecular characterization of PPV strains. An epidemiological survey was conducted on 1534 aborted fetuses from the southwestern region between 2020 and 2023, revealing an abortion rate of 3.00% due to PPV2, with the highest rate of 3.77% in Sichuan. Additionally, 2973 blood samples from sows were tested using ELISA, showing a PPV2 antibody positivity rate of 73.03% to 90%. Through shotgun metagenomics, PPV2 SC2020 was identified in aborted fetal samples from a pig farm in Pengzhou, Sichuan. PCR sequencing analysis yielded seven PPV2 genomic sequences, and the phylogenetic analysis of eight PPV2 strains with thirty reference strains showed distinct evolutionary branches. The virus was successfully isolated from PPV2-positive samples, and the phylogenetic analysis of PPV2 SC2020 revealed ORF1 gene homology of 94.9% to 99.3% and the ORF2 gene homology of 93.1% to 98.0%, with 34 reference strains. Homologous recombination analysis indicated that SC2020 is a recombinant strain of HeB03 and S1.https://www.mdpi.com/2306-7381/12/2/99PPV2high-throughput analysissow miscarriagerecombinant strain |
| spellingShingle | Hongyu Chen Yi Qing Lei Xu Ling Zhu Wenqi Yin Shuwei Li Shengyao Kuang Yuancheng Zhou Zhiwen Xu Prevalence and Molecular Characterization of Porcine Parvovirus 2 in Southwest China During 2020–2023 Veterinary Sciences PPV2 high-throughput analysis sow miscarriage recombinant strain |
| title | Prevalence and Molecular Characterization of Porcine Parvovirus 2 in Southwest China During 2020–2023 |
| title_full | Prevalence and Molecular Characterization of Porcine Parvovirus 2 in Southwest China During 2020–2023 |
| title_fullStr | Prevalence and Molecular Characterization of Porcine Parvovirus 2 in Southwest China During 2020–2023 |
| title_full_unstemmed | Prevalence and Molecular Characterization of Porcine Parvovirus 2 in Southwest China During 2020–2023 |
| title_short | Prevalence and Molecular Characterization of Porcine Parvovirus 2 in Southwest China During 2020–2023 |
| title_sort | prevalence and molecular characterization of porcine parvovirus 2 in southwest china during 2020 2023 |
| topic | PPV2 high-throughput analysis sow miscarriage recombinant strain |
| url | https://www.mdpi.com/2306-7381/12/2/99 |
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