EXPERIMENTAL ANALYSIS IN WISTAR RATS OF BONE GRAFTS PREPARED BY DIFFERENT STERILIZATION METHODS
ABSTRACT Objectives: To evaluate in vitro the bone graft preparations sterilization (BG) by freezing, gamma irradiation and ethylene oxide and compare in vivo their osseointegration in Wistar rat femurs. Method: This basic controlled experimental study carried out in two phases, in vitro. First, i...
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Sociedade Brasileira de Ortopedia e Traumatologia
2025-08-01
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| Series: | Acta Ortopédica Brasileira |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-78522025000301750&lng=en&tlng=en |
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| author | Pedro Gabriel Minikowski Martins Luan Praxedes de Oliveira Thiago Mota dos Santos André Vinícius Silva Müller Erick Leonardo Martins Nascimento Juliano Valerio Bortolletto |
| author_facet | Pedro Gabriel Minikowski Martins Luan Praxedes de Oliveira Thiago Mota dos Santos André Vinícius Silva Müller Erick Leonardo Martins Nascimento Juliano Valerio Bortolletto |
| author_sort | Pedro Gabriel Minikowski Martins |
| collection | DOAJ |
| description | ABSTRACT Objectives: To evaluate in vitro the bone graft preparations sterilization (BG) by freezing, gamma irradiation and ethylene oxide and compare in vivo their osseointegration in Wistar rat femurs. Method: This basic controlled experimental study carried out in two phases, in vitro. First, in vitro, it was evaluated the sterilization of bone allografts exposed to S. aureus, A. baumanii, M. tuberculosis and C. albicans. In the second phase, in vivo, bone allografts osteointegration was compared in thirty-two Wistar rats, separated into four groups. In the control group (CG), fenestrations were carried out in the femur and not given a graft. Other groups received bone allografts prepared by freezing to –70ºC, ethylene oxide and 25KGy gamma irradiation. After thirty days, femurs were histologically evaluated by Hematoxylin-Eosin and Alcian Blue colorations and the Fisher's Exact test was used with p≤0.05. Results: In the in vitro study was observed lower level of decontamination by freezing method (26.6±21.09%) meanwhile and the other methods being 100% efficient. In the in vivo study, we observed no significant differences between the inflammatory reaction, mineralization and osteointegration. The percentage of proteoglycans was higher in the Gamma Irradiation Group and Ethylene Oxide Group. However, there were no significant differences. In the Frozen Graft Group, there was an absence in 37.5% of proteoglycans. Conclusion: The preparation of the bone allografts by freezing is less effective than the other methods and there were no differences in the osteointegration of the bone allografts in rat femurs. |
| format | Article |
| id | doaj-art-ca8523a035a3498dbaf24cd23a08d745 |
| institution | DOAJ |
| issn | 1413-7852 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Sociedade Brasileira de Ortopedia e Traumatologia |
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| series | Acta Ortopédica Brasileira |
| spelling | doaj-art-ca8523a035a3498dbaf24cd23a08d7452025-08-20T03:07:28ZengSociedade Brasileira de Ortopedia e TraumatologiaActa Ortopédica Brasileira1413-78522025-08-0133310.1590/1413-785220253303e289865EXPERIMENTAL ANALYSIS IN WISTAR RATS OF BONE GRAFTS PREPARED BY DIFFERENT STERILIZATION METHODSPedro Gabriel Minikowski Martinshttps://orcid.org/0009-0009-6727-726XLuan Praxedes de Oliveirahttps://orcid.org/0009-0009-1823-5645Thiago Mota dos Santoshttps://orcid.org/0009-0001-7221-4820André Vinícius Silva Müllerhttps://orcid.org/0009-0000-9068-4394Erick Leonardo Martins Nascimentohttps://orcid.org/0009-0001-4082-3971Juliano Valerio Bortollettohttps://orcid.org/0009-0000-7305-1069ABSTRACT Objectives: To evaluate in vitro the bone graft preparations sterilization (BG) by freezing, gamma irradiation and ethylene oxide and compare in vivo their osseointegration in Wistar rat femurs. Method: This basic controlled experimental study carried out in two phases, in vitro. First, in vitro, it was evaluated the sterilization of bone allografts exposed to S. aureus, A. baumanii, M. tuberculosis and C. albicans. In the second phase, in vivo, bone allografts osteointegration was compared in thirty-two Wistar rats, separated into four groups. In the control group (CG), fenestrations were carried out in the femur and not given a graft. Other groups received bone allografts prepared by freezing to –70ºC, ethylene oxide and 25KGy gamma irradiation. After thirty days, femurs were histologically evaluated by Hematoxylin-Eosin and Alcian Blue colorations and the Fisher's Exact test was used with p≤0.05. Results: In the in vitro study was observed lower level of decontamination by freezing method (26.6±21.09%) meanwhile and the other methods being 100% efficient. In the in vivo study, we observed no significant differences between the inflammatory reaction, mineralization and osteointegration. The percentage of proteoglycans was higher in the Gamma Irradiation Group and Ethylene Oxide Group. However, there were no significant differences. In the Frozen Graft Group, there was an absence in 37.5% of proteoglycans. Conclusion: The preparation of the bone allografts by freezing is less effective than the other methods and there were no differences in the osteointegration of the bone allografts in rat femurs.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-78522025000301750&lng=en&tlng=enRatsSterilizationFreezingIrradiationEthylene OxideProteoglycans |
| spellingShingle | Pedro Gabriel Minikowski Martins Luan Praxedes de Oliveira Thiago Mota dos Santos André Vinícius Silva Müller Erick Leonardo Martins Nascimento Juliano Valerio Bortolletto EXPERIMENTAL ANALYSIS IN WISTAR RATS OF BONE GRAFTS PREPARED BY DIFFERENT STERILIZATION METHODS Acta Ortopédica Brasileira Rats Sterilization Freezing Irradiation Ethylene Oxide Proteoglycans |
| title | EXPERIMENTAL ANALYSIS IN WISTAR RATS OF BONE GRAFTS PREPARED BY DIFFERENT STERILIZATION METHODS |
| title_full | EXPERIMENTAL ANALYSIS IN WISTAR RATS OF BONE GRAFTS PREPARED BY DIFFERENT STERILIZATION METHODS |
| title_fullStr | EXPERIMENTAL ANALYSIS IN WISTAR RATS OF BONE GRAFTS PREPARED BY DIFFERENT STERILIZATION METHODS |
| title_full_unstemmed | EXPERIMENTAL ANALYSIS IN WISTAR RATS OF BONE GRAFTS PREPARED BY DIFFERENT STERILIZATION METHODS |
| title_short | EXPERIMENTAL ANALYSIS IN WISTAR RATS OF BONE GRAFTS PREPARED BY DIFFERENT STERILIZATION METHODS |
| title_sort | experimental analysis in wistar rats of bone grafts prepared by different sterilization methods |
| topic | Rats Sterilization Freezing Irradiation Ethylene Oxide Proteoglycans |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-78522025000301750&lng=en&tlng=en |
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