The Peptide-Encoding <i>CLE25</i> Gene Modulates Drought Response in Cotton
CLAVATA3 (CLV3)/endosperm surrounding region (CLE) peptides have been reportedly involved in plant growth and development, as well as responses to abiotic stresses. However, the stress resilience of most <i>CLE</i> genes in cotton remains largely unknown. Here, induced expression pattern...
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MDPI AG
2025-06-01
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| author | Dayong Zhang Qingfeng Zhu Pu Qin Lu Yu Weixi Li Hao Sun |
| author_facet | Dayong Zhang Qingfeng Zhu Pu Qin Lu Yu Weixi Li Hao Sun |
| author_sort | Dayong Zhang |
| collection | DOAJ |
| description | CLAVATA3 (CLV3)/endosperm surrounding region (CLE) peptides have been reportedly involved in plant growth and development, as well as responses to abiotic stresses. However, the stress resilience of most <i>CLE</i> genes in cotton remains largely unknown. Here, induced expression pattern analysis showed that <i>GhCLE25</i> was obviously responsive to osmotic and salt treatments, indicating that <i>GhCLE25</i> was involved in abiotic stress tolerance. Furthermore, silencing <i>GhCLE25</i> or the exogenous application of CLE25p effectively led to reduced and enhanced drought tolerance, respectively, as indicated by the activities of the plants’ POD, SOD, CAT, and MDA contents, as well as their height and fresh weight. We found that the knockdown of <i>GhCLE25</i> promoted seedling growth and development, with a higher plant height and fresh weight in <i>GhCLE25</i>-silenced plants in comparison to control plants. In addition, a comparative transcriptome analysis of TRV:00 versus TRV:<i>GhCLE25</i> and Mock versus CLE25p revealed that the CLE25-mediated signaling pathway is mainly involved in defense response and phytohormone signaling. Collectively, these findings indicate diverse roles of CLE25 in regulating plant growth and response to environmental stimuli and highlight the potential utilization of CLE25 to improve drought stress in modern agriculture via CLE25p spraying. |
| format | Article |
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| language | English |
| publishDate | 2025-06-01 |
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| spelling | doaj-art-ca8510f0946e4c9d8a9adf62f832b7732025-08-20T03:10:50ZengMDPI AGAgriculture2077-04722025-06-011511122610.3390/agriculture15111226The Peptide-Encoding <i>CLE25</i> Gene Modulates Drought Response in CottonDayong Zhang0Qingfeng Zhu1Pu Qin2Lu Yu3Weixi Li4Hao Sun5State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Engineering Research Center of Ministry of Education for Cotton Germplasm Enhancement and Application, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Engineering Research Center of Ministry of Education for Cotton Germplasm Enhancement and Application, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Engineering Research Center of Ministry of Education for Cotton Germplasm Enhancement and Application, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Engineering Research Center of Ministry of Education for Cotton Germplasm Enhancement and Application, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaCLAVATA3 (CLV3)/endosperm surrounding region (CLE) peptides have been reportedly involved in plant growth and development, as well as responses to abiotic stresses. However, the stress resilience of most <i>CLE</i> genes in cotton remains largely unknown. Here, induced expression pattern analysis showed that <i>GhCLE25</i> was obviously responsive to osmotic and salt treatments, indicating that <i>GhCLE25</i> was involved in abiotic stress tolerance. Furthermore, silencing <i>GhCLE25</i> or the exogenous application of CLE25p effectively led to reduced and enhanced drought tolerance, respectively, as indicated by the activities of the plants’ POD, SOD, CAT, and MDA contents, as well as their height and fresh weight. We found that the knockdown of <i>GhCLE25</i> promoted seedling growth and development, with a higher plant height and fresh weight in <i>GhCLE25</i>-silenced plants in comparison to control plants. In addition, a comparative transcriptome analysis of TRV:00 versus TRV:<i>GhCLE25</i> and Mock versus CLE25p revealed that the CLE25-mediated signaling pathway is mainly involved in defense response and phytohormone signaling. Collectively, these findings indicate diverse roles of CLE25 in regulating plant growth and response to environmental stimuli and highlight the potential utilization of CLE25 to improve drought stress in modern agriculture via CLE25p spraying.https://www.mdpi.com/2077-0472/15/11/1226cotton<i>GhCLE25</i>exogenous sprayingdrought responseRNA-seq |
| spellingShingle | Dayong Zhang Qingfeng Zhu Pu Qin Lu Yu Weixi Li Hao Sun The Peptide-Encoding <i>CLE25</i> Gene Modulates Drought Response in Cotton Agriculture cotton <i>GhCLE25</i> exogenous spraying drought response RNA-seq |
| title | The Peptide-Encoding <i>CLE25</i> Gene Modulates Drought Response in Cotton |
| title_full | The Peptide-Encoding <i>CLE25</i> Gene Modulates Drought Response in Cotton |
| title_fullStr | The Peptide-Encoding <i>CLE25</i> Gene Modulates Drought Response in Cotton |
| title_full_unstemmed | The Peptide-Encoding <i>CLE25</i> Gene Modulates Drought Response in Cotton |
| title_short | The Peptide-Encoding <i>CLE25</i> Gene Modulates Drought Response in Cotton |
| title_sort | peptide encoding i cle25 i gene modulates drought response in cotton |
| topic | cotton <i>GhCLE25</i> exogenous spraying drought response RNA-seq |
| url | https://www.mdpi.com/2077-0472/15/11/1226 |
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