Cell–Cell Interaction of Macrophages and Vascular Smooth Muscle Cells in the Synthesis of Leukotriene B4
Biosynthesis of LTB4 during cell-cell interaction between vascular smooth muscle cells (SMC) and alveolar macrophages (AM) has been investigated by use of both high-pressure Hquid chromatography (HPLC) and radtoimmunoassay (RIA). Both interleukin-β (IL-β) and tumour necrosis factor-α (TNFα) induced...
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| Format: | Article |
| Language: | English |
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Wiley
1994-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935194000414 |
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| _version_ | 1832559999358861312 |
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| author | M. Zou C. Anges |
| author_facet | M. Zou C. Anges |
| author_sort | M. Zou |
| collection | DOAJ |
| description | Biosynthesis of LTB4 during cell-cell interaction between
vascular smooth muscle cells (SMC) and alveolar macrophages (AM) has
been investigated by use of both high-pressure Hquid chromatography
(HPLC) and radtoimmunoassay (RIA). Both interleukin-β
(IL-β) and tumour necrosis factor-α (TNFα) induced
a time- and dose-dependent synthesis of 15-, and
5-hydroxyeicosatetraenoic acids (HETEs) from cultured SMC. However,
neither TNFα nor IL-1β induced a significant
LTB4 production in SMC alone or AM alone after 24 h of
incubation. Addition of IL-1β and TNFα simultaneously to
SMC resulted in a dose-dependent synergistic increase of HETEs.
Macrophages dose-dependently transformed extremely low
concentrations of exogenous LTA4 into LTB4.
Incubation of vascular SMC with various numbers of AM in the
presence of IL-1β (5 units/ml) and TNFα (10
units/ml) induced a great increase of LTB4 synthesis
in comparison with the detectable levels of LTB4 produced
by macrophages alone. Pretreatment of SMC with NDGA, cycloheximide,
and actinomycin not only inhibited IL-1 and TNT induced HETEs
synthesis but also abolished LTB4 production when
co-incubated with macrophages. These results suggest that
LTB4 in a mixture of SMC and macrophages could originate
from a transcellular metabolism, i.e. macrophages transforming
SMC-derived LTA4 into LTB4. |
| format | Article |
| id | doaj-art-ca778c2994f949da8939792503e26be9 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1994-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-ca778c2994f949da8939792503e26be92025-02-03T01:28:42ZengWileyMediators of Inflammation0962-93511466-18611994-01-013429730210.1155/S0962935194000414Cell–Cell Interaction of Macrophages and Vascular Smooth Muscle Cells in the Synthesis of Leukotriene B4M. Zou0C. Anges1Laboratoire d'lmmunopharmacologie, Institut des Cordeliers, 15 rue de I'école de Mddecine, Paris 75270, FranceLaboratoire de Biochimie, Hôpital Broussais, Paris 75674, FranceBiosynthesis of LTB4 during cell-cell interaction between vascular smooth muscle cells (SMC) and alveolar macrophages (AM) has been investigated by use of both high-pressure Hquid chromatography (HPLC) and radtoimmunoassay (RIA). Both interleukin-β (IL-β) and tumour necrosis factor-α (TNFα) induced a time- and dose-dependent synthesis of 15-, and 5-hydroxyeicosatetraenoic acids (HETEs) from cultured SMC. However, neither TNFα nor IL-1β induced a significant LTB4 production in SMC alone or AM alone after 24 h of incubation. Addition of IL-1β and TNFα simultaneously to SMC resulted in a dose-dependent synergistic increase of HETEs. Macrophages dose-dependently transformed extremely low concentrations of exogenous LTA4 into LTB4. Incubation of vascular SMC with various numbers of AM in the presence of IL-1β (5 units/ml) and TNFα (10 units/ml) induced a great increase of LTB4 synthesis in comparison with the detectable levels of LTB4 produced by macrophages alone. Pretreatment of SMC with NDGA, cycloheximide, and actinomycin not only inhibited IL-1 and TNT induced HETEs synthesis but also abolished LTB4 production when co-incubated with macrophages. These results suggest that LTB4 in a mixture of SMC and macrophages could originate from a transcellular metabolism, i.e. macrophages transforming SMC-derived LTA4 into LTB4.http://dx.doi.org/10.1155/S0962935194000414 |
| spellingShingle | M. Zou C. Anges Cell–Cell Interaction of Macrophages and Vascular Smooth Muscle Cells in the Synthesis of Leukotriene B4 Mediators of Inflammation |
| title | Cell–Cell Interaction of Macrophages and Vascular Smooth Muscle
Cells in the Synthesis of Leukotriene B4 |
| title_full | Cell–Cell Interaction of Macrophages and Vascular Smooth Muscle
Cells in the Synthesis of Leukotriene B4 |
| title_fullStr | Cell–Cell Interaction of Macrophages and Vascular Smooth Muscle
Cells in the Synthesis of Leukotriene B4 |
| title_full_unstemmed | Cell–Cell Interaction of Macrophages and Vascular Smooth Muscle
Cells in the Synthesis of Leukotriene B4 |
| title_short | Cell–Cell Interaction of Macrophages and Vascular Smooth Muscle
Cells in the Synthesis of Leukotriene B4 |
| title_sort | cell cell interaction of macrophages and vascular smooth muscle cells in the synthesis of leukotriene b4 |
| url | http://dx.doi.org/10.1155/S0962935194000414 |
| work_keys_str_mv | AT mzou cellcellinteractionofmacrophagesandvascularsmoothmusclecellsinthesynthesisofleukotrieneb4 AT canges cellcellinteractionofmacrophagesandvascularsmoothmusclecellsinthesynthesisofleukotrieneb4 |