Establishment of a clinical workflow for in vivo Raman spectroscopy during head and neck cancer surgery
Abstract As first part of an ongoing prospective feasibility trial (DRKS00028114) this work explored the integration of in vivo Raman spectroscopy (RS) into the routine setting workflow of head and neck cancer (HNC) surgery. In vivo RS was performed intraoperatively on 30 patients with HNC cell carc...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-025-08222-9 |
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| author | Ayman Bali Thomas Bitter Mussab Kouka Jonas Ballmaier Ines Latka Florian Windirsch David Pertzborn Nadja Ziller Marcela Mafra Nikolaus Gaßler Jürgen Popp Anna Mühlig Ferdinand von Eggeling Orlando Guntinas-Lichius Iwan W. Schie |
| author_facet | Ayman Bali Thomas Bitter Mussab Kouka Jonas Ballmaier Ines Latka Florian Windirsch David Pertzborn Nadja Ziller Marcela Mafra Nikolaus Gaßler Jürgen Popp Anna Mühlig Ferdinand von Eggeling Orlando Guntinas-Lichius Iwan W. Schie |
| author_sort | Ayman Bali |
| collection | DOAJ |
| description | Abstract As first part of an ongoing prospective feasibility trial (DRKS00028114) this work explored the integration of in vivo Raman spectroscopy (RS) into the routine setting workflow of head and neck cancer (HNC) surgery. In vivo RS was performed intraoperatively on 30 patients with HNC cell carcinoma and 10 patients with inflammatory diseases as a control group. A standardized process was established using a Raman system complied with stringent medical device regulatory standards. Spectra were collected in vivo from the tumor site, the tumor margins, and healthy tissue. The learning curve of the HNC team significantly improved measurement times from over 30 min initially to 2 min after 15 patients. Data from 35 patients were interpretable, demonstrating clear spectral differences between tumor and healthy tissues. The intraoperative in vivo RS workflow is now well established and is being used in the ongoing clinical trial. |
| format | Article |
| id | doaj-art-ca6eaece26cc4f3d88a8aaf876009610 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-ca6eaece26cc4f3d88a8aaf8760096102025-08-20T03:42:57ZengNature PortfolioScientific Reports2045-23222025-07-0115111210.1038/s41598-025-08222-9Establishment of a clinical workflow for in vivo Raman spectroscopy during head and neck cancer surgeryAyman Bali0Thomas Bitter1Mussab Kouka2Jonas Ballmaier3Ines Latka4Florian Windirsch5David Pertzborn6Nadja Ziller7Marcela Mafra8Nikolaus Gaßler9Jürgen Popp10Anna Mühlig11Ferdinand von Eggeling12Orlando Guntinas-Lichius13Iwan W. Schie14Department of Otorhinolaryngology, Jena University Hospital DepartmentDepartment of Otorhinolaryngology, Jena University Hospital DepartmentDepartment of Otorhinolaryngology, Jena University Hospital DepartmentDepartment of Otorhinolaryngology, Jena University Hospital DepartmentLeibniz Institute of Photonic TechnologyLeibniz Institute of Photonic TechnologyDepartment of Otorhinolaryngology, Jena University Hospital DepartmentDepartment of Otorhinolaryngology, Jena University Hospital DepartmentDepartment of Otorhinolaryngology, Jena University Hospital DepartmentSection Pathology of the Institute of Forensic Medicine, Jena University HospitalLeibniz Institute of Photonic TechnologyDepartment of Otorhinolaryngology, Jena University Hospital DepartmentDepartment of Otorhinolaryngology, Jena University Hospital DepartmentDepartment of Otorhinolaryngology, Jena University Hospital DepartmentLeibniz Institute of Photonic TechnologyAbstract As first part of an ongoing prospective feasibility trial (DRKS00028114) this work explored the integration of in vivo Raman spectroscopy (RS) into the routine setting workflow of head and neck cancer (HNC) surgery. In vivo RS was performed intraoperatively on 30 patients with HNC cell carcinoma and 10 patients with inflammatory diseases as a control group. A standardized process was established using a Raman system complied with stringent medical device regulatory standards. Spectra were collected in vivo from the tumor site, the tumor margins, and healthy tissue. The learning curve of the HNC team significantly improved measurement times from over 30 min initially to 2 min after 15 patients. Data from 35 patients were interpretable, demonstrating clear spectral differences between tumor and healthy tissues. The intraoperative in vivo RS workflow is now well established and is being used in the ongoing clinical trial.https://doi.org/10.1038/s41598-025-08222-9 |
| spellingShingle | Ayman Bali Thomas Bitter Mussab Kouka Jonas Ballmaier Ines Latka Florian Windirsch David Pertzborn Nadja Ziller Marcela Mafra Nikolaus Gaßler Jürgen Popp Anna Mühlig Ferdinand von Eggeling Orlando Guntinas-Lichius Iwan W. Schie Establishment of a clinical workflow for in vivo Raman spectroscopy during head and neck cancer surgery Scientific Reports |
| title | Establishment of a clinical workflow for in vivo Raman spectroscopy during head and neck cancer surgery |
| title_full | Establishment of a clinical workflow for in vivo Raman spectroscopy during head and neck cancer surgery |
| title_fullStr | Establishment of a clinical workflow for in vivo Raman spectroscopy during head and neck cancer surgery |
| title_full_unstemmed | Establishment of a clinical workflow for in vivo Raman spectroscopy during head and neck cancer surgery |
| title_short | Establishment of a clinical workflow for in vivo Raman spectroscopy during head and neck cancer surgery |
| title_sort | establishment of a clinical workflow for in vivo raman spectroscopy during head and neck cancer surgery |
| url | https://doi.org/10.1038/s41598-025-08222-9 |
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