Investigating the effects of rosmarinic acid on ovarian tissue, inflammatory markers, and sex hormones in polycystic ovary syndrome rats
Abstract Polycystic ovary syndrome (PCOS) causes the impairment of female fertility and elevates the risk of metabolic disorders. The current study aimed to evaluate the effects of rosmarinic acid (Ros) on the ovarian histo‐stereology, the level of reproductive hormones, and inflammation in a rat mo...
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Wiley
2025-04-01
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| Series: | Physiological Reports |
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| Online Access: | https://doi.org/10.14814/phy2.70304 |
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| author | Sina Vakili Farhad Koohpeyma Mohammad Samare‐Najaf Bahia Namavar Jahromi Morteza Jafarinia Sepide Goharitaban Amir Savardashtaki Ali Samareh Fatemeh Amini Mohammad Hashem Hashempur |
| author_facet | Sina Vakili Farhad Koohpeyma Mohammad Samare‐Najaf Bahia Namavar Jahromi Morteza Jafarinia Sepide Goharitaban Amir Savardashtaki Ali Samareh Fatemeh Amini Mohammad Hashem Hashempur |
| author_sort | Sina Vakili |
| collection | DOAJ |
| description | Abstract Polycystic ovary syndrome (PCOS) causes the impairment of female fertility and elevates the risk of metabolic disorders. The current study aimed to evaluate the effects of rosmarinic acid (Ros) on the ovarian histo‐stereology, the level of reproductive hormones, and inflammation in a rat model of PCOS. Fifteen adult Sprague Dawley rats were randomly divided into three groups, including controls, PCOS, and PCOS+Ros (receiving 25 mg/kg of Ros for 39 days). After treatments, the ovarian histo‐stereology, the level of reproductive hormones, and the level of inflammatory markers were analyzed. PCOS led to increased ovarian weight and volume, cortical and medullary expansion, reduced ovarian follicles, and enhanced follicular atresia. It also caused hormonal imbalances, elevating LH, FSH, and testosterone while decreasing estradiol, progesterone, and AMH. Additionally, PCOS increased pro‐inflammatory markers (TNF‐α and IL‐6) and decreased anti‐inflammatory markers (IL‐4 and IL‐10). However, Ros administration in PCOS animals improved ovarian structure, increased follicle numbers, reduced atresia, balanced reproductive hormones, and restored inflammatory markers (p value <0.05). The present findings may suggest Ros as a novel strategy for the management of PCOS, although further studies are necessary. |
| format | Article |
| id | doaj-art-ca6502aba9514324af4351ea88c58e77 |
| institution | OA Journals |
| issn | 2051-817X |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | Physiological Reports |
| spelling | doaj-art-ca6502aba9514324af4351ea88c58e772025-08-20T02:12:38ZengWileyPhysiological Reports2051-817X2025-04-01137n/an/a10.14814/phy2.70304Investigating the effects of rosmarinic acid on ovarian tissue, inflammatory markers, and sex hormones in polycystic ovary syndrome ratsSina Vakili0Farhad Koohpeyma1Mohammad Samare‐Najaf2Bahia Namavar Jahromi3Morteza Jafarinia4Sepide Goharitaban5Amir Savardashtaki6Ali Samareh7Fatemeh Amini8Mohammad Hashem Hashempur9Infertility Research Center Shiraz University of Medical Sciences Shiraz IranEndocrinology and Metabolism Research Center Shiraz University of Medical Science Shiraz IranBlood Transfusion Research Center High Institute for Research and Education in Transfusion Medicine Tehran IranInfertility Research Center Shiraz University of Medical Sciences Shiraz IranShiraz Neuroscience Research Center Shiraz University of Medical Sciences Shiraz IranDepartment of Anatomical Sciences, Faculty of Medicine Hamedan University of Medical Sciences Hamedan IranInfertility Research Center Shiraz University of Medical Sciences Shiraz IranDepartment of Clinical Biochemistry, School of Medicine Kerman University of Medical Sciences Kerman IranDepartment of Persian Medicine and Pharmacy, School of Pharmacy Ahvaz Jundishapur University of Medical Sciences Ahvaz IranResearch Center for Traditional Medicine and History of Medicine, Department of Persian Medicine, School of Medicine Shiraz University of Medical Sciences Shiraz IranAbstract Polycystic ovary syndrome (PCOS) causes the impairment of female fertility and elevates the risk of metabolic disorders. The current study aimed to evaluate the effects of rosmarinic acid (Ros) on the ovarian histo‐stereology, the level of reproductive hormones, and inflammation in a rat model of PCOS. Fifteen adult Sprague Dawley rats were randomly divided into three groups, including controls, PCOS, and PCOS+Ros (receiving 25 mg/kg of Ros for 39 days). After treatments, the ovarian histo‐stereology, the level of reproductive hormones, and the level of inflammatory markers were analyzed. PCOS led to increased ovarian weight and volume, cortical and medullary expansion, reduced ovarian follicles, and enhanced follicular atresia. It also caused hormonal imbalances, elevating LH, FSH, and testosterone while decreasing estradiol, progesterone, and AMH. Additionally, PCOS increased pro‐inflammatory markers (TNF‐α and IL‐6) and decreased anti‐inflammatory markers (IL‐4 and IL‐10). However, Ros administration in PCOS animals improved ovarian structure, increased follicle numbers, reduced atresia, balanced reproductive hormones, and restored inflammatory markers (p value <0.05). The present findings may suggest Ros as a novel strategy for the management of PCOS, although further studies are necessary.https://doi.org/10.14814/phy2.70304histologyhormoneinfertilityintegrative medicinephytotherapypolycystic ovary syndrome |
| spellingShingle | Sina Vakili Farhad Koohpeyma Mohammad Samare‐Najaf Bahia Namavar Jahromi Morteza Jafarinia Sepide Goharitaban Amir Savardashtaki Ali Samareh Fatemeh Amini Mohammad Hashem Hashempur Investigating the effects of rosmarinic acid on ovarian tissue, inflammatory markers, and sex hormones in polycystic ovary syndrome rats Physiological Reports histology hormone infertility integrative medicine phytotherapy polycystic ovary syndrome |
| title | Investigating the effects of rosmarinic acid on ovarian tissue, inflammatory markers, and sex hormones in polycystic ovary syndrome rats |
| title_full | Investigating the effects of rosmarinic acid on ovarian tissue, inflammatory markers, and sex hormones in polycystic ovary syndrome rats |
| title_fullStr | Investigating the effects of rosmarinic acid on ovarian tissue, inflammatory markers, and sex hormones in polycystic ovary syndrome rats |
| title_full_unstemmed | Investigating the effects of rosmarinic acid on ovarian tissue, inflammatory markers, and sex hormones in polycystic ovary syndrome rats |
| title_short | Investigating the effects of rosmarinic acid on ovarian tissue, inflammatory markers, and sex hormones in polycystic ovary syndrome rats |
| title_sort | investigating the effects of rosmarinic acid on ovarian tissue inflammatory markers and sex hormones in polycystic ovary syndrome rats |
| topic | histology hormone infertility integrative medicine phytotherapy polycystic ovary syndrome |
| url | https://doi.org/10.14814/phy2.70304 |
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