The key target and role of betel nut in oral squamous cell carcinoma

Abstract Background Betel nut consumption is closely related to oral cancer. This study aimed to explore the crucial target and role of betel nut in oral cancer. Methods We used RNA sequencing and protein-protein interaction networks to screen the key target of betel nut in oral cancer. Enrichment a...

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Main Authors: Tianyi Zhang, Haofeng Xiong, Liujun Zeng, Zhimin Yang, Xin Hu, Tong Su
Format: Article
Language:English
Published: BMC 2025-07-01
Series:BMC Oral Health
Subjects:
Online Access:https://doi.org/10.1186/s12903-025-06558-2
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author Tianyi Zhang
Haofeng Xiong
Liujun Zeng
Zhimin Yang
Xin Hu
Tong Su
author_facet Tianyi Zhang
Haofeng Xiong
Liujun Zeng
Zhimin Yang
Xin Hu
Tong Su
author_sort Tianyi Zhang
collection DOAJ
description Abstract Background Betel nut consumption is closely related to oral cancer. This study aimed to explore the crucial target and role of betel nut in oral cancer. Methods We used RNA sequencing and protein-protein interaction networks to screen the key target of betel nut in oral cancer. Enrichment analysis was used to explore the role of betel nut. Immunohistochemical analysis was used to identify the expression trend of the key target, and survival analysis was used to explore the prognostic value of the key target. The animal model of oral cancer was used to verify the key target of betel nut. Results Firstly, 1108 genes were differentially expressed in oral cancer under the influence of betel nut, which may be involved in “metabolic pathways.” Moreover, TP53 was the key target of betel nut and was upregulated, resulting in poor prognosis in patients with oral cancer. Finally, the differential expression and role of TP53 in oral cancer with betel nut were validated by human and animal samples. Conclusions In conclusion, we found that TP53 upregulation may be the crucial biological phenomenon in the oral cancer with betel nut, indicating that TP53 may be the key target of betel nut in oral cancer.
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publishDate 2025-07-01
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series BMC Oral Health
spelling doaj-art-ca581b86849a4d2aaebfc6a003abd4322025-08-20T03:42:07ZengBMCBMC Oral Health1472-68312025-07-0125111010.1186/s12903-025-06558-2The key target and role of betel nut in oral squamous cell carcinomaTianyi Zhang0Haofeng Xiong1Liujun Zeng2Zhimin Yang3Xin Hu4Tong Su5Department of Oral and Maxillofacial Surgery, Center of Stomatology, Xiangya Hospital, Central South UniversityDepartment of Oral and Maxillofacial Surgery, Center of Stomatology, Xiangya Hospital, Central South UniversityDepartment of Oral and Maxillofacial Surgery, Center of Stomatology, Xiangya Hospital, Central South UniversityDepartment of Oral and Maxillofacial Surgery, Center of Stomatology, Xiangya Hospital, Central South UniversityDepartment of Oral and Maxillofacial Surgery, Center of Stomatology, Xiangya Hospital, Central South UniversityDepartment of Oral and Maxillofacial Surgery, Center of Stomatology, Xiangya Hospital, Central South UniversityAbstract Background Betel nut consumption is closely related to oral cancer. This study aimed to explore the crucial target and role of betel nut in oral cancer. Methods We used RNA sequencing and protein-protein interaction networks to screen the key target of betel nut in oral cancer. Enrichment analysis was used to explore the role of betel nut. Immunohistochemical analysis was used to identify the expression trend of the key target, and survival analysis was used to explore the prognostic value of the key target. The animal model of oral cancer was used to verify the key target of betel nut. Results Firstly, 1108 genes were differentially expressed in oral cancer under the influence of betel nut, which may be involved in “metabolic pathways.” Moreover, TP53 was the key target of betel nut and was upregulated, resulting in poor prognosis in patients with oral cancer. Finally, the differential expression and role of TP53 in oral cancer with betel nut were validated by human and animal samples. Conclusions In conclusion, we found that TP53 upregulation may be the crucial biological phenomenon in the oral cancer with betel nut, indicating that TP53 may be the key target of betel nut in oral cancer.https://doi.org/10.1186/s12903-025-06558-2Oral squamous cell carcinomaBetel nutTP53
spellingShingle Tianyi Zhang
Haofeng Xiong
Liujun Zeng
Zhimin Yang
Xin Hu
Tong Su
The key target and role of betel nut in oral squamous cell carcinoma
BMC Oral Health
Oral squamous cell carcinoma
Betel nut
TP53
title The key target and role of betel nut in oral squamous cell carcinoma
title_full The key target and role of betel nut in oral squamous cell carcinoma
title_fullStr The key target and role of betel nut in oral squamous cell carcinoma
title_full_unstemmed The key target and role of betel nut in oral squamous cell carcinoma
title_short The key target and role of betel nut in oral squamous cell carcinoma
title_sort key target and role of betel nut in oral squamous cell carcinoma
topic Oral squamous cell carcinoma
Betel nut
TP53
url https://doi.org/10.1186/s12903-025-06558-2
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