An inexpensive point-of-care immunochromatographic test for Talaromyces marneffei infection based on the yeast phase specific monoclonal antibody 4D1 and Galanthus nivalis agglutinin.
Talaromyces marneffei is a thermally dimorphic fungus that causes opportunistic systemic mycoses in patients with AIDS or other immunodeficiency syndromes. The purpose of this study was to develop an immunochromatographic strip test (ICT) based on a solid phase sandwich format immunoassay for the de...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-05-01
|
| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0009058&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850046241889583104 |
|---|---|
| author | Kritsada Pruksaphon Akarin Intaramat Pavinee Simsiriwong Skorn Mongkolsuk Kavi Ratanabanangkoon Joshua D Nosanchuk Anna Kaltsas Sirida Youngchim |
| author_facet | Kritsada Pruksaphon Akarin Intaramat Pavinee Simsiriwong Skorn Mongkolsuk Kavi Ratanabanangkoon Joshua D Nosanchuk Anna Kaltsas Sirida Youngchim |
| author_sort | Kritsada Pruksaphon |
| collection | DOAJ |
| description | Talaromyces marneffei is a thermally dimorphic fungus that causes opportunistic systemic mycoses in patients with AIDS or other immunodeficiency syndromes. The purpose of this study was to develop an immunochromatographic strip test (ICT) based on a solid phase sandwich format immunoassay for the detection of T. marneffei antigens in clinical urine specimens. The T. marneffei yeast phase specific monoclonal antibody 4D1 (MAb4D1) conjugated with colloidal gold nanoparticle was used as a specific signal reporter. Galanthus nivalis Agglutinin (GNA) was adsorbed onto nitrocellulose membrane to serve as the test line. Similarly, a control line was created above the test line by immobilization of rabbit anti-mouse IgG. The immobilized GNA served as capturing molecule and as non-immune mediated anti-terminal mannose of T. marneffei antigenic mannoprotein. The MAb4D1-GNA based ICT showed specific binding activity with yeast phase antigen of T. marneffei, and it did not react with other common pathogenic fungal antigens. The limit of detection of this ICT for T. marneffei antigen spiked in normal urine was approximately 0.6 μg/ml. The diagnostic performance of the ICT was validated using 341 urine samples from patents with culture- confirmed T. marneffei infection and from a control group of healthy individuals and patients with other infections in an endemic area. The ICT exhibited 89.47% sensitivity, 100% specificity, and 97.65% accuracy. Our results demonstrate that the urine-based GNA-MAb4D1 based ICT produces a visual result within 30 minutes and that the test is highly specific for the diagnosis of T. marneffei infection. The findings validate the deployment of the ICT for clinical use. |
| format | Article |
| id | doaj-art-ca576bc5769643c88a9698bf890fa5b8 |
| institution | DOAJ |
| issn | 1935-2727 1935-2735 |
| language | English |
| publishDate | 2021-05-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj-art-ca576bc5769643c88a9698bf890fa5b82025-08-20T02:54:29ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352021-05-01155e000905810.1371/journal.pntd.0009058An inexpensive point-of-care immunochromatographic test for Talaromyces marneffei infection based on the yeast phase specific monoclonal antibody 4D1 and Galanthus nivalis agglutinin.Kritsada PruksaphonAkarin IntaramatPavinee SimsiriwongSkorn MongkolsukKavi RatanabanangkoonJoshua D NosanchukAnna KaltsasSirida YoungchimTalaromyces marneffei is a thermally dimorphic fungus that causes opportunistic systemic mycoses in patients with AIDS or other immunodeficiency syndromes. The purpose of this study was to develop an immunochromatographic strip test (ICT) based on a solid phase sandwich format immunoassay for the detection of T. marneffei antigens in clinical urine specimens. The T. marneffei yeast phase specific monoclonal antibody 4D1 (MAb4D1) conjugated with colloidal gold nanoparticle was used as a specific signal reporter. Galanthus nivalis Agglutinin (GNA) was adsorbed onto nitrocellulose membrane to serve as the test line. Similarly, a control line was created above the test line by immobilization of rabbit anti-mouse IgG. The immobilized GNA served as capturing molecule and as non-immune mediated anti-terminal mannose of T. marneffei antigenic mannoprotein. The MAb4D1-GNA based ICT showed specific binding activity with yeast phase antigen of T. marneffei, and it did not react with other common pathogenic fungal antigens. The limit of detection of this ICT for T. marneffei antigen spiked in normal urine was approximately 0.6 μg/ml. The diagnostic performance of the ICT was validated using 341 urine samples from patents with culture- confirmed T. marneffei infection and from a control group of healthy individuals and patients with other infections in an endemic area. The ICT exhibited 89.47% sensitivity, 100% specificity, and 97.65% accuracy. Our results demonstrate that the urine-based GNA-MAb4D1 based ICT produces a visual result within 30 minutes and that the test is highly specific for the diagnosis of T. marneffei infection. The findings validate the deployment of the ICT for clinical use.https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0009058&type=printable |
| spellingShingle | Kritsada Pruksaphon Akarin Intaramat Pavinee Simsiriwong Skorn Mongkolsuk Kavi Ratanabanangkoon Joshua D Nosanchuk Anna Kaltsas Sirida Youngchim An inexpensive point-of-care immunochromatographic test for Talaromyces marneffei infection based on the yeast phase specific monoclonal antibody 4D1 and Galanthus nivalis agglutinin. PLoS Neglected Tropical Diseases |
| title | An inexpensive point-of-care immunochromatographic test for Talaromyces marneffei infection based on the yeast phase specific monoclonal antibody 4D1 and Galanthus nivalis agglutinin. |
| title_full | An inexpensive point-of-care immunochromatographic test for Talaromyces marneffei infection based on the yeast phase specific monoclonal antibody 4D1 and Galanthus nivalis agglutinin. |
| title_fullStr | An inexpensive point-of-care immunochromatographic test for Talaromyces marneffei infection based on the yeast phase specific monoclonal antibody 4D1 and Galanthus nivalis agglutinin. |
| title_full_unstemmed | An inexpensive point-of-care immunochromatographic test for Talaromyces marneffei infection based on the yeast phase specific monoclonal antibody 4D1 and Galanthus nivalis agglutinin. |
| title_short | An inexpensive point-of-care immunochromatographic test for Talaromyces marneffei infection based on the yeast phase specific monoclonal antibody 4D1 and Galanthus nivalis agglutinin. |
| title_sort | inexpensive point of care immunochromatographic test for talaromyces marneffei infection based on the yeast phase specific monoclonal antibody 4d1 and galanthus nivalis agglutinin |
| url | https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0009058&type=printable |
| work_keys_str_mv | AT kritsadapruksaphon aninexpensivepointofcareimmunochromatographictestfortalaromycesmarneffeiinfectionbasedontheyeastphasespecificmonoclonalantibody4d1andgalanthusnivalisagglutinin AT akarinintaramat aninexpensivepointofcareimmunochromatographictestfortalaromycesmarneffeiinfectionbasedontheyeastphasespecificmonoclonalantibody4d1andgalanthusnivalisagglutinin AT pavineesimsiriwong aninexpensivepointofcareimmunochromatographictestfortalaromycesmarneffeiinfectionbasedontheyeastphasespecificmonoclonalantibody4d1andgalanthusnivalisagglutinin AT skornmongkolsuk aninexpensivepointofcareimmunochromatographictestfortalaromycesmarneffeiinfectionbasedontheyeastphasespecificmonoclonalantibody4d1andgalanthusnivalisagglutinin AT kaviratanabanangkoon aninexpensivepointofcareimmunochromatographictestfortalaromycesmarneffeiinfectionbasedontheyeastphasespecificmonoclonalantibody4d1andgalanthusnivalisagglutinin AT joshuadnosanchuk aninexpensivepointofcareimmunochromatographictestfortalaromycesmarneffeiinfectionbasedontheyeastphasespecificmonoclonalantibody4d1andgalanthusnivalisagglutinin AT annakaltsas aninexpensivepointofcareimmunochromatographictestfortalaromycesmarneffeiinfectionbasedontheyeastphasespecificmonoclonalantibody4d1andgalanthusnivalisagglutinin AT siridayoungchim aninexpensivepointofcareimmunochromatographictestfortalaromycesmarneffeiinfectionbasedontheyeastphasespecificmonoclonalantibody4d1andgalanthusnivalisagglutinin AT kritsadapruksaphon inexpensivepointofcareimmunochromatographictestfortalaromycesmarneffeiinfectionbasedontheyeastphasespecificmonoclonalantibody4d1andgalanthusnivalisagglutinin AT akarinintaramat inexpensivepointofcareimmunochromatographictestfortalaromycesmarneffeiinfectionbasedontheyeastphasespecificmonoclonalantibody4d1andgalanthusnivalisagglutinin AT pavineesimsiriwong inexpensivepointofcareimmunochromatographictestfortalaromycesmarneffeiinfectionbasedontheyeastphasespecificmonoclonalantibody4d1andgalanthusnivalisagglutinin AT skornmongkolsuk inexpensivepointofcareimmunochromatographictestfortalaromycesmarneffeiinfectionbasedontheyeastphasespecificmonoclonalantibody4d1andgalanthusnivalisagglutinin AT kaviratanabanangkoon inexpensivepointofcareimmunochromatographictestfortalaromycesmarneffeiinfectionbasedontheyeastphasespecificmonoclonalantibody4d1andgalanthusnivalisagglutinin AT joshuadnosanchuk inexpensivepointofcareimmunochromatographictestfortalaromycesmarneffeiinfectionbasedontheyeastphasespecificmonoclonalantibody4d1andgalanthusnivalisagglutinin AT annakaltsas inexpensivepointofcareimmunochromatographictestfortalaromycesmarneffeiinfectionbasedontheyeastphasespecificmonoclonalantibody4d1andgalanthusnivalisagglutinin AT siridayoungchim inexpensivepointofcareimmunochromatographictestfortalaromycesmarneffeiinfectionbasedontheyeastphasespecificmonoclonalantibody4d1andgalanthusnivalisagglutinin |