Succinic Acid Production from Monosaccharides and Woody and Herbaceous Plant Hydrolysates Using Metabolically Engineered Corynebacterium glutamicum§
Research background. Succinic acid from lignocellulosic biomass is a sustainable alternative for biochemical production that is an environmentally friendly substitute for petroleum-based chemicals. The aim of this study is to evaluate the effects of variations in hemicellulose content and cellulose...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Zagreb Faculty of Food Technology and Biotechnology
2025-01-01
|
| Series: | Food Technology and Biotechnology |
| Subjects: | |
| Online Access: | https://hrcak.srce.hr/file/482595 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850112630542303232 |
|---|---|
| author | Dae-Seok Lee Eun Jin Cho Seryung Kim Dien Thanh Nguyen Hyeun-Jong Bae |
| author_facet | Dae-Seok Lee Eun Jin Cho Seryung Kim Dien Thanh Nguyen Hyeun-Jong Bae |
| author_sort | Dae-Seok Lee |
| collection | DOAJ |
| description | Research background. Succinic acid from lignocellulosic biomass is a sustainable alternative for biochemical production that is an environmentally friendly substitute for petroleum-based chemicals. The aim of this study is to evaluate the effects of variations in hemicellulose content and cellulose fibre structure within the microfibrils of woody and herbaceous plants on the enzymatic saccharification and succinic acid production efficiency of Psod:SucE12-ΔldhA, a strain overexpressing the succinic acid transporter (SucE).
Experimental approach. The study investigated the influence of different monosaccharide combinations on succinic acid production, focusing on combinations with mannose compared to glucose alone. Additionally, hydrolysates from different lignocellulosic biomass — bamboo, oak, poplar, pine and spent coffee grounds — were analysed to determine the most favourable bioresource for succinic acid production.
Results and conclusions. Monosaccharide combinations containing mannose resulted in 2.20-2.48 times higher succinic acid production than glucose alone, indicating a positive influence of mannose on succinic acid metabolism. Among the lignocellulosic biomass hydrolysates, bamboo, with its higher xylose content than woody plants, was the most efficient bioresource for succinic acid production (23.38–24.12 g/L within 24 h), followed by oak, poplar, pine and spent coffee grounds. Therefore, improving the xylose consumption rate is crucial for increasing succinic acid production from lignocellulosic biomass and increasing market competitiveness.
Novelty and scientific contribution. This research emphasises the potential of lignocellulosic biomass, especially bamboo, as a sustainable feedstock for succinic acid production. The novelty of the study lies in the detailed investigation of how hemicellulose content and cellulose fibre structure affect enzymatic saccharification and fermentation. The significant influence of mannose and xylose on the succinic acid yield provides key insights for the optimisation of biomass use in biochemical production. These findings promote bio-based chemical production, reduce reliance on fossil fuels and improve industrial sustainability. |
| format | Article |
| id | doaj-art-ca520dff34dc4f3aa2afc712d6ee519b |
| institution | OA Journals |
| issn | 1330-9862 1334-2606 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
| record_format | Article |
| series | Food Technology and Biotechnology |
| spelling | doaj-art-ca520dff34dc4f3aa2afc712d6ee519b2025-08-20T02:37:20ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062025-01-0163213414810.17113/ftb.63.02.25.8808Succinic Acid Production from Monosaccharides and Woody and Herbaceous Plant Hydrolysates Using Metabolically Engineered Corynebacterium glutamicum§Dae-Seok Lee0Eun Jin Cho1Seryung Kim2Dien Thanh Nguyen3Hyeun-Jong Bae4Bio-Energy Research Institute, Chonnam National University, Gwangju 61186, Republic of KoreaBio-Energy Research Institute, Chonnam National University, Gwangju 61186, Republic of KoreaDepartment of Integrative Food, Bioscience and Biotechnology, Chonnam National University, Gwangju 61186, Republic of KoreaSchool of Biotechnology, Tan Tao University, Long An 82000, Viet NamBio-Energy Research Institute, Chonnam National University, Gwangju 61186, Republic of KoreaResearch background. Succinic acid from lignocellulosic biomass is a sustainable alternative for biochemical production that is an environmentally friendly substitute for petroleum-based chemicals. The aim of this study is to evaluate the effects of variations in hemicellulose content and cellulose fibre structure within the microfibrils of woody and herbaceous plants on the enzymatic saccharification and succinic acid production efficiency of Psod:SucE12-ΔldhA, a strain overexpressing the succinic acid transporter (SucE). Experimental approach. The study investigated the influence of different monosaccharide combinations on succinic acid production, focusing on combinations with mannose compared to glucose alone. Additionally, hydrolysates from different lignocellulosic biomass — bamboo, oak, poplar, pine and spent coffee grounds — were analysed to determine the most favourable bioresource for succinic acid production. Results and conclusions. Monosaccharide combinations containing mannose resulted in 2.20-2.48 times higher succinic acid production than glucose alone, indicating a positive influence of mannose on succinic acid metabolism. Among the lignocellulosic biomass hydrolysates, bamboo, with its higher xylose content than woody plants, was the most efficient bioresource for succinic acid production (23.38–24.12 g/L within 24 h), followed by oak, poplar, pine and spent coffee grounds. Therefore, improving the xylose consumption rate is crucial for increasing succinic acid production from lignocellulosic biomass and increasing market competitiveness. Novelty and scientific contribution. This research emphasises the potential of lignocellulosic biomass, especially bamboo, as a sustainable feedstock for succinic acid production. The novelty of the study lies in the detailed investigation of how hemicellulose content and cellulose fibre structure affect enzymatic saccharification and fermentation. The significant influence of mannose and xylose on the succinic acid yield provides key insights for the optimisation of biomass use in biochemical production. These findings promote bio-based chemical production, reduce reliance on fossil fuels and improve industrial sustainability.https://hrcak.srce.hr/file/482595succinic acidlignocellulosic biomassbambooenzymatic saccharificationfermentation |
| spellingShingle | Dae-Seok Lee Eun Jin Cho Seryung Kim Dien Thanh Nguyen Hyeun-Jong Bae Succinic Acid Production from Monosaccharides and Woody and Herbaceous Plant Hydrolysates Using Metabolically Engineered Corynebacterium glutamicum§ Food Technology and Biotechnology succinic acid lignocellulosic biomass bamboo enzymatic saccharification fermentation |
| title | Succinic Acid Production from Monosaccharides and Woody and Herbaceous Plant Hydrolysates Using Metabolically Engineered Corynebacterium glutamicum§ |
| title_full | Succinic Acid Production from Monosaccharides and Woody and Herbaceous Plant Hydrolysates Using Metabolically Engineered Corynebacterium glutamicum§ |
| title_fullStr | Succinic Acid Production from Monosaccharides and Woody and Herbaceous Plant Hydrolysates Using Metabolically Engineered Corynebacterium glutamicum§ |
| title_full_unstemmed | Succinic Acid Production from Monosaccharides and Woody and Herbaceous Plant Hydrolysates Using Metabolically Engineered Corynebacterium glutamicum§ |
| title_short | Succinic Acid Production from Monosaccharides and Woody and Herbaceous Plant Hydrolysates Using Metabolically Engineered Corynebacterium glutamicum§ |
| title_sort | succinic acid production from monosaccharides and woody and herbaceous plant hydrolysates using metabolically engineered corynebacterium glutamicum§ |
| topic | succinic acid lignocellulosic biomass bamboo enzymatic saccharification fermentation |
| url | https://hrcak.srce.hr/file/482595 |
| work_keys_str_mv | AT daeseoklee succinicacidproductionfrommonosaccharidesandwoodyandherbaceousplanthydrolysatesusingmetabolicallyengineeredcorynebacteriumglutamicum AT eunjincho succinicacidproductionfrommonosaccharidesandwoodyandherbaceousplanthydrolysatesusingmetabolicallyengineeredcorynebacteriumglutamicum AT seryungkim succinicacidproductionfrommonosaccharidesandwoodyandherbaceousplanthydrolysatesusingmetabolicallyengineeredcorynebacteriumglutamicum AT dienthanhnguyen succinicacidproductionfrommonosaccharidesandwoodyandherbaceousplanthydrolysatesusingmetabolicallyengineeredcorynebacteriumglutamicum AT hyeunjongbae succinicacidproductionfrommonosaccharidesandwoodyandherbaceousplanthydrolysatesusingmetabolicallyengineeredcorynebacteriumglutamicum |