Phylogenetic Analysis of Streptomyces spp. Isolated from Soil Samples in Sulaimani Governorate
Streptomyces species have been an important source of bioactive secondary metabolites and clinically useful for antibiotic production, including fosfomycin, daptomycin, oxytetracycline, streptomycin, and chloramphenicol. The main objective of this study was to isolate actinomycetes, especially Strep...
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Erbil Polytechnic University
2020-06-01
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| Online Access: | https://polytechnic-journal.epu.edu.iq/home/vol10/iss1/4 |
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| author | Syamand A. Qadir Miran H. Qadir Osama H. Shareef Aryan M. Faraj |
| author_facet | Syamand A. Qadir Miran H. Qadir Osama H. Shareef Aryan M. Faraj |
| author_sort | Syamand A. Qadir |
| collection | DOAJ |
| description | Streptomyces species have been an important source of bioactive secondary metabolites and clinically
useful for antibiotic production, including fosfomycin, daptomycin, oxytetracycline, streptomycin, and
chloramphenicol. The main objective of this study was to isolate actinomycetes, especially Streptomyces
from 8 soil samples that collected from two different places, which are districts in Sulaimani governorate,
Kurdistan Region of Iraq. Totally, 30 diagnostic tests were carried out for representative of isolated
Streptomyces depending on the color groups. The 16Sr DNA gene was sequenced, for ten isolated
test strains were amplified with 2 universal primers after extraction of genomic DNA, only 8 of them
were successfully sequenced. Phylogenetic analysis of 8 test strains carried out using base sequences
of 16Sr DNA genes in the core genome. Four of isolated test strains were identified as a Streptomyces
fulvissimus, and others were nominated as a Streptomyces anulatus. The obtained sequence data were
compared with the sequence data of the closest related species in the international databases using
EzTaxon Server program and (%) similarities were determined. Finally, phylogenetic analysis indicated
that isolated test strains were nominated as (H001, H002, H003, and H004), which recognized members
of the S. anulatus and the similarity with their type strain is 99.93%. Whereas, other four isolated
test strains, including D001, D002, D003, and D004 recognized as members of the S. fulvissimus
and similarity with their type strain is 100%. |
| format | Article |
| id | doaj-art-ca51cebde05244ddb51fd1398b2a282c |
| institution | DOAJ |
| issn | 2707-7799 |
| language | English |
| publishDate | 2020-06-01 |
| publisher | Erbil Polytechnic University |
| record_format | Article |
| series | Polytechnic Journal |
| spelling | doaj-art-ca51cebde05244ddb51fd1398b2a282c2025-08-20T03:05:06ZengErbil Polytechnic UniversityPolytechnic Journal2707-77992020-06-011011824https://doi.org/10.25156/ptj.v10n1y2020.pp18-24Phylogenetic Analysis of Streptomyces spp. Isolated from Soil Samples in Sulaimani GovernorateSyamand A. Qadir0Miran H. Qadir1Osama H. Shareef2Aryan M. Faraj3Department of Medical Laboratory Science, Technical College of Applied Sciences, Sulaimani Polytechnic University, Sulaymaniyah, Kurdistan Region, Iraq, Department of Horticulture, Collage of Agricultural Engineering Sciences, University of Raparin, Sulaymaniyah, Kurdistan Region, IraqDepartment of Medical Laboratory Science, Technical College of Applied Sciences, Sulaimani Polytechnic University, Sulaymaniyah, Kurdistan Region, Iraq, Department of Medical Laboratory Science, Technical College of Applied Sciences, Sulaimani Polytechnic University, Sulaymaniyah, Kurdistan Region, Iraq, Streptomyces species have been an important source of bioactive secondary metabolites and clinically useful for antibiotic production, including fosfomycin, daptomycin, oxytetracycline, streptomycin, and chloramphenicol. The main objective of this study was to isolate actinomycetes, especially Streptomyces from 8 soil samples that collected from two different places, which are districts in Sulaimani governorate, Kurdistan Region of Iraq. Totally, 30 diagnostic tests were carried out for representative of isolated Streptomyces depending on the color groups. The 16Sr DNA gene was sequenced, for ten isolated test strains were amplified with 2 universal primers after extraction of genomic DNA, only 8 of them were successfully sequenced. Phylogenetic analysis of 8 test strains carried out using base sequences of 16Sr DNA genes in the core genome. Four of isolated test strains were identified as a Streptomyces fulvissimus, and others were nominated as a Streptomyces anulatus. The obtained sequence data were compared with the sequence data of the closest related species in the international databases using EzTaxon Server program and (%) similarities were determined. Finally, phylogenetic analysis indicated that isolated test strains were nominated as (H001, H002, H003, and H004), which recognized members of the S. anulatus and the similarity with their type strain is 99.93%. Whereas, other four isolated test strains, including D001, D002, D003, and D004 recognized as members of the S. fulvissimus and similarity with their type strain is 100%.https://polytechnic-journal.epu.edu.iq/home/vol10/iss1/4actinobacteria;streptomyces;antibiotic;16sr dna and diaminopimelic acid;soil |
| spellingShingle | Syamand A. Qadir Miran H. Qadir Osama H. Shareef Aryan M. Faraj Phylogenetic Analysis of Streptomyces spp. Isolated from Soil Samples in Sulaimani Governorate Polytechnic Journal actinobacteria; streptomyces; antibiotic; 16sr dna and diaminopimelic acid; soil |
| title | Phylogenetic Analysis of Streptomyces spp. Isolated from Soil Samples in Sulaimani Governorate |
| title_full | Phylogenetic Analysis of Streptomyces spp. Isolated from Soil Samples in Sulaimani Governorate |
| title_fullStr | Phylogenetic Analysis of Streptomyces spp. Isolated from Soil Samples in Sulaimani Governorate |
| title_full_unstemmed | Phylogenetic Analysis of Streptomyces spp. Isolated from Soil Samples in Sulaimani Governorate |
| title_short | Phylogenetic Analysis of Streptomyces spp. Isolated from Soil Samples in Sulaimani Governorate |
| title_sort | phylogenetic analysis of streptomyces spp isolated from soil samples in sulaimani governorate |
| topic | actinobacteria; streptomyces; antibiotic; 16sr dna and diaminopimelic acid; soil |
| url | https://polytechnic-journal.epu.edu.iq/home/vol10/iss1/4 |
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