A novel protein encoded by porcine circANKRD17 activates the PPAR pathway to regulate intramuscular fat metabolism
Abstract Background Intramuscular fat is an important factor in evaluating pork quality and varies widely among different pig breeds. However, the regulatory mechanism of circular RNAs (circRNAs) in lipid metabolism remains largely unexplored. Results We combined circRNA-seq and Ribo-seq data to scr...
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BMC
2025-02-01
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| Series: | Journal of Animal Science and Biotechnology |
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| Online Access: | https://doi.org/10.1186/s40104-025-01153-5 |
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| author | Xiao He Fang Xie Ying Nie Xuefeng Wang Junyi Luo Ting Chen Qianyun Xi Yongliang Zhang Jiajie Sun |
| author_facet | Xiao He Fang Xie Ying Nie Xuefeng Wang Junyi Luo Ting Chen Qianyun Xi Yongliang Zhang Jiajie Sun |
| author_sort | Xiao He |
| collection | DOAJ |
| description | Abstract Background Intramuscular fat is an important factor in evaluating pork quality and varies widely among different pig breeds. However, the regulatory mechanism of circular RNAs (circRNAs) in lipid metabolism remains largely unexplored. Results We combined circRNA-seq and Ribo-seq data to screen a total of 18 circRNA candidates with coding potential, and circANKRD17 was found to be significantly elevated in the longissimus dorsi muscle of Lantang piglets, with a length of 1,844 nucleotides. Using single-cell sequencing, we identified 477 differentially expressed genes in IMF cells between Lantang and Landrace piglets, with enrichment in the PPAR signaling pathway. These genes included FABP4, FABP5, CPT1A, and UBC, consistent with the high levels of acylcarnitines observed in the longissimus dorsi muscles of the Lantang breed, as determined by lipidomic analysis. Further in vitro and in vivo experiments indicated that circANKRD17 can regulate lipid metabolism through various mechanisms involving the PPAR pathway, including promoting adipocyte differentiation, fatty acid transport and metabolism, triglyceride synthesis, and lipid droplet formation and maturation. In addition, we discovered that circANKRD17 has an open reading frame and can be translated into a novel 571-amino-acid protein that promotes lipid metabolism. Conclusions Our research provides new insights into the role of protein-coding circANKRD17, especially concerning the metabolic characteristics of pig breeds with higher intramuscular fat content. |
| format | Article |
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| institution | Kabale University |
| issn | 2049-1891 |
| language | English |
| publishDate | 2025-02-01 |
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| series | Journal of Animal Science and Biotechnology |
| spelling | doaj-art-ca491bdca23f4c1288ecae06482820542025-02-09T12:49:06ZengBMCJournal of Animal Science and Biotechnology2049-18912025-02-0116111610.1186/s40104-025-01153-5A novel protein encoded by porcine circANKRD17 activates the PPAR pathway to regulate intramuscular fat metabolismXiao He0Fang Xie1Ying Nie2Xuefeng Wang3Junyi Luo4Ting Chen5Qianyun Xi6Yongliang Zhang7Jiajie Sun8Guangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural UniversityGuangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural UniversityGuangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural UniversityGuangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural UniversityGuangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural UniversityGuangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural UniversityGuangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural UniversityGuangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural UniversityGuangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural UniversityAbstract Background Intramuscular fat is an important factor in evaluating pork quality and varies widely among different pig breeds. However, the regulatory mechanism of circular RNAs (circRNAs) in lipid metabolism remains largely unexplored. Results We combined circRNA-seq and Ribo-seq data to screen a total of 18 circRNA candidates with coding potential, and circANKRD17 was found to be significantly elevated in the longissimus dorsi muscle of Lantang piglets, with a length of 1,844 nucleotides. Using single-cell sequencing, we identified 477 differentially expressed genes in IMF cells between Lantang and Landrace piglets, with enrichment in the PPAR signaling pathway. These genes included FABP4, FABP5, CPT1A, and UBC, consistent with the high levels of acylcarnitines observed in the longissimus dorsi muscles of the Lantang breed, as determined by lipidomic analysis. Further in vitro and in vivo experiments indicated that circANKRD17 can regulate lipid metabolism through various mechanisms involving the PPAR pathway, including promoting adipocyte differentiation, fatty acid transport and metabolism, triglyceride synthesis, and lipid droplet formation and maturation. In addition, we discovered that circANKRD17 has an open reading frame and can be translated into a novel 571-amino-acid protein that promotes lipid metabolism. Conclusions Our research provides new insights into the role of protein-coding circANKRD17, especially concerning the metabolic characteristics of pig breeds with higher intramuscular fat content.https://doi.org/10.1186/s40104-025-01153-5CircRNAsIntramuscular fatMeat qualityPPAR pathway |
| spellingShingle | Xiao He Fang Xie Ying Nie Xuefeng Wang Junyi Luo Ting Chen Qianyun Xi Yongliang Zhang Jiajie Sun A novel protein encoded by porcine circANKRD17 activates the PPAR pathway to regulate intramuscular fat metabolism Journal of Animal Science and Biotechnology CircRNAs Intramuscular fat Meat quality PPAR pathway |
| title | A novel protein encoded by porcine circANKRD17 activates the PPAR pathway to regulate intramuscular fat metabolism |
| title_full | A novel protein encoded by porcine circANKRD17 activates the PPAR pathway to regulate intramuscular fat metabolism |
| title_fullStr | A novel protein encoded by porcine circANKRD17 activates the PPAR pathway to regulate intramuscular fat metabolism |
| title_full_unstemmed | A novel protein encoded by porcine circANKRD17 activates the PPAR pathway to regulate intramuscular fat metabolism |
| title_short | A novel protein encoded by porcine circANKRD17 activates the PPAR pathway to regulate intramuscular fat metabolism |
| title_sort | novel protein encoded by porcine circankrd17 activates the ppar pathway to regulate intramuscular fat metabolism |
| topic | CircRNAs Intramuscular fat Meat quality PPAR pathway |
| url | https://doi.org/10.1186/s40104-025-01153-5 |
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