Exploration of Potential Roles of a New LOXL2 Splicing Variant Using Network Knowledge in Esophageal Squamous Cell Carcinoma
LOXL2 (lysyl oxidase-like 2), an enzyme that catalyzes oxidative deamination of lysine residue, is upregulated in esophageal squamous cell carcinoma (ESCC). A LOXL2 splice variant LOXL2-e13 and its wild type were overexpressed in ESCC cells followed by microarray analyses. In this study, we explored...
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2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/431792 |
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| author | Bing-Li Wu Guo-Qing Lv Hai-Ying Zou Ze-Peng Du Jian-Yi Wu Pi-Xian Zhang Li-Yan Xu En-Min Li |
| author_facet | Bing-Li Wu Guo-Qing Lv Hai-Ying Zou Ze-Peng Du Jian-Yi Wu Pi-Xian Zhang Li-Yan Xu En-Min Li |
| author_sort | Bing-Li Wu |
| collection | DOAJ |
| description | LOXL2 (lysyl oxidase-like 2), an enzyme that catalyzes oxidative deamination of lysine residue, is upregulated in esophageal squamous cell carcinoma (ESCC). A LOXL2 splice variant LOXL2-e13 and its wild type were overexpressed in ESCC cells followed by microarray analyses. In this study, we explored the potential role and molecular mechanism of LOXL2-e13 based on known protein-protein interactions (PPIs), following microarray analysis of KYSE150 ESCC cells overexpressing a LOXL2 splice variant, denoted by LOXL2-e13, or its wild-type counterpart. The differentially expressed genes (DEGs) of LOXL2-WT and LOXL2-e13 were applied to generate individual PPI subnetworks in which hundreds of DEGs interacted with thousands of other proteins. These two DEG groups were annotated by Functional Annotation Chart analysis in the DAVID bioinformatics database and compared. These results found many specific annotations indicating the potential specific role or mechanism for LOXL2-e13. The DEGs of LOXL2-e13, comparing to its wild type, were prioritized by the Random Walk with Restart algorithm. Several tumor-related genes such as ERO1L, ITGA3, and MAPK8 were found closest to LOXL2-e13. These results provide helpful information for subsequent experimental identification of the specific biological roles and molecular mechanisms of LOXL2-e13. Our study also provides a work flow to identify potential roles of splice variants with large scale data. |
| format | Article |
| id | doaj-art-ca47da59f63945ccba7b3d73c937cd5a |
| institution | DOAJ |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-ca47da59f63945ccba7b3d73c937cd5a2025-08-20T03:19:43ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/431792431792Exploration of Potential Roles of a New LOXL2 Splicing Variant Using Network Knowledge in Esophageal Squamous Cell CarcinomaBing-Li Wu0Guo-Qing Lv1Hai-Ying Zou2Ze-Peng Du3Jian-Yi Wu4Pi-Xian Zhang5Li-Yan Xu6En-Min Li7Department of Biochemistry and Molecular Biology, Shantou University Medical College, Shantou, Guangdong 515041, ChinaDepartment of Biochemistry and Molecular Biology, Shantou University Medical College, Shantou, Guangdong 515041, ChinaDepartment of Biochemistry and Molecular Biology, Shantou University Medical College, Shantou, Guangdong 515041, ChinaDepartment of Pathology, Shantou Central Hospital, Affiliated Shantou Hospital of Sun Yat-sen University, Shantou, Guangdong 515041, ChinaDepartment of Biochemistry and Molecular Biology, Shantou University Medical College, Shantou, Guangdong 515041, ChinaDepartment of Biochemistry and Molecular Biology, Shantou University Medical College, Shantou, Guangdong 515041, ChinaInstitute of Oncologic Pathology, Shantou University Medical College, Shantou, Guangdong 515041, ChinaDepartment of Biochemistry and Molecular Biology, Shantou University Medical College, Shantou, Guangdong 515041, ChinaLOXL2 (lysyl oxidase-like 2), an enzyme that catalyzes oxidative deamination of lysine residue, is upregulated in esophageal squamous cell carcinoma (ESCC). A LOXL2 splice variant LOXL2-e13 and its wild type were overexpressed in ESCC cells followed by microarray analyses. In this study, we explored the potential role and molecular mechanism of LOXL2-e13 based on known protein-protein interactions (PPIs), following microarray analysis of KYSE150 ESCC cells overexpressing a LOXL2 splice variant, denoted by LOXL2-e13, or its wild-type counterpart. The differentially expressed genes (DEGs) of LOXL2-WT and LOXL2-e13 were applied to generate individual PPI subnetworks in which hundreds of DEGs interacted with thousands of other proteins. These two DEG groups were annotated by Functional Annotation Chart analysis in the DAVID bioinformatics database and compared. These results found many specific annotations indicating the potential specific role or mechanism for LOXL2-e13. The DEGs of LOXL2-e13, comparing to its wild type, were prioritized by the Random Walk with Restart algorithm. Several tumor-related genes such as ERO1L, ITGA3, and MAPK8 were found closest to LOXL2-e13. These results provide helpful information for subsequent experimental identification of the specific biological roles and molecular mechanisms of LOXL2-e13. Our study also provides a work flow to identify potential roles of splice variants with large scale data.http://dx.doi.org/10.1155/2014/431792 |
| spellingShingle | Bing-Li Wu Guo-Qing Lv Hai-Ying Zou Ze-Peng Du Jian-Yi Wu Pi-Xian Zhang Li-Yan Xu En-Min Li Exploration of Potential Roles of a New LOXL2 Splicing Variant Using Network Knowledge in Esophageal Squamous Cell Carcinoma The Scientific World Journal |
| title | Exploration of Potential Roles of a New LOXL2 Splicing Variant Using Network Knowledge in Esophageal Squamous Cell Carcinoma |
| title_full | Exploration of Potential Roles of a New LOXL2 Splicing Variant Using Network Knowledge in Esophageal Squamous Cell Carcinoma |
| title_fullStr | Exploration of Potential Roles of a New LOXL2 Splicing Variant Using Network Knowledge in Esophageal Squamous Cell Carcinoma |
| title_full_unstemmed | Exploration of Potential Roles of a New LOXL2 Splicing Variant Using Network Knowledge in Esophageal Squamous Cell Carcinoma |
| title_short | Exploration of Potential Roles of a New LOXL2 Splicing Variant Using Network Knowledge in Esophageal Squamous Cell Carcinoma |
| title_sort | exploration of potential roles of a new loxl2 splicing variant using network knowledge in esophageal squamous cell carcinoma |
| url | http://dx.doi.org/10.1155/2014/431792 |
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