Methodological aspects of diagnostics and minimal residual disease monitoring in infant acute leukemias
Hereby we present methodological aspects and prognostic significance of minimal residual disease (MRD) monitoring in infant acute leukemias. Based on our own experience we made algorithm for detection of MRD in this group of patients. We conclude that general concordance between MRD detection by flo...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
ABV-press
2016-03-01
|
| Series: | Онкогематология |
| Subjects: | |
| Online Access: | https://oncohematology.abvpress.ru/ongm/article/view/184 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849689956960698368 |
|---|---|
| author | G. A. Tsaur A. M. Popov L. G. Fechina S. A. Rumyantsev |
| author_facet | G. A. Tsaur A. M. Popov L. G. Fechina S. A. Rumyantsev |
| author_sort | G. A. Tsaur |
| collection | DOAJ |
| description | Hereby we present methodological aspects and prognostic significance of minimal residual disease (MRD) monitoring in infant acute leukemias. Based on our own experience we made algorithm for detection of MRD in this group of patients. We conclude that general concordance between MRD detection by flow cytometry and real-time polymerase chain reaction (PCR) for fusion gene transcripts achieved 87.0 %. Concordance was significantly lower during induction in comparison to consolidation/intensification and relapse treatment (78.6; 90.4 and 93.4 %, correspondingly; p = 0.002). It was not dependent on presence of normal B-cell precursors. Concordance between MRD results obtained by qualitative real-time PCR in bone marrow and peripheral blood samples was 84.5 %. Interestingly, all discrepant results (22 samples 15.5 %) were MRD-positive in bone marrow, but negative in peripheral blood. Despite high qualitative concordance rate between MRD detection in bone marrow and peripheral blood samples we could not show prognostic value of MRD monitoring in peripheral blood by fusion gene transcripts. Multivariate analysis revealed that MRD-positivity at time-point 4 in bone marrow was the only significant and independent prognostic factor of unfavorable outcome in the observed group of patients (hazard ratio 7.326; 95 % confidence interval 2.378–22.565). |
| format | Article |
| id | doaj-art-ca411649a07a4dd487a5fc577366e509 |
| institution | DOAJ |
| issn | 1818-8346 2413-4023 |
| language | Russian |
| publishDate | 2016-03-01 |
| publisher | ABV-press |
| record_format | Article |
| series | Онкогематология |
| spelling | doaj-art-ca411649a07a4dd487a5fc577366e5092025-08-20T03:21:27ZrusABV-pressОнкогематология1818-83462413-40232016-03-01111627410.17650/1818-8346-2016-11-1-62-74192Methodological aspects of diagnostics and minimal residual disease monitoring in infant acute leukemiasG. A. Tsaur0A. M. Popov1L. G. Fechina2S. A. Rumyantsev3Regional Children Clinical Hospital No 1; 32 S. Deryabinoy St., Ekaterinburg, 620149, Russia Institute of Medical Cell Technologies; 22a Karla Marksa St., Ekaterinburg, 620026, Russia Ural Federal University named after the first President of Russia B. N. Yel’tsin; 19 Mira St., Ekaterinburg, 620002, RussiaFederal Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitriy Rogachev, Ministry of Health of Russia; 1 Samory Mashela St., Moscow, 117998, RussiaRegional Children Clinical Hospital No 1; 32 S. Deryabinoy St., Ekaterinburg, 620149, RussiaFederal Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitriy Rogachev, Ministry of Health of Russia; 1 Samory Mashela St., Moscow, 117998, Russia N.I. Pirogov Russian National Research Medical University, Ministry of Health of Russia; 1 Ostrovityanova St., Moscow, 117997, RussiaHereby we present methodological aspects and prognostic significance of minimal residual disease (MRD) monitoring in infant acute leukemias. Based on our own experience we made algorithm for detection of MRD in this group of patients. We conclude that general concordance between MRD detection by flow cytometry and real-time polymerase chain reaction (PCR) for fusion gene transcripts achieved 87.0 %. Concordance was significantly lower during induction in comparison to consolidation/intensification and relapse treatment (78.6; 90.4 and 93.4 %, correspondingly; p = 0.002). It was not dependent on presence of normal B-cell precursors. Concordance between MRD results obtained by qualitative real-time PCR in bone marrow and peripheral blood samples was 84.5 %. Interestingly, all discrepant results (22 samples 15.5 %) were MRD-positive in bone marrow, but negative in peripheral blood. Despite high qualitative concordance rate between MRD detection in bone marrow and peripheral blood samples we could not show prognostic value of MRD monitoring in peripheral blood by fusion gene transcripts. Multivariate analysis revealed that MRD-positivity at time-point 4 in bone marrow was the only significant and independent prognostic factor of unfavorable outcome in the observed group of patients (hazard ratio 7.326; 95 % confidence interval 2.378–22.565).https://oncohematology.abvpress.ru/ongm/article/view/18411q23/mll rearrangementsinfant acute leukemiasminimal residual disease |
| spellingShingle | G. A. Tsaur A. M. Popov L. G. Fechina S. A. Rumyantsev Methodological aspects of diagnostics and minimal residual disease monitoring in infant acute leukemias Онкогематология 11q23/mll rearrangements infant acute leukemias minimal residual disease |
| title | Methodological aspects of diagnostics and minimal residual disease monitoring in infant acute leukemias |
| title_full | Methodological aspects of diagnostics and minimal residual disease monitoring in infant acute leukemias |
| title_fullStr | Methodological aspects of diagnostics and minimal residual disease monitoring in infant acute leukemias |
| title_full_unstemmed | Methodological aspects of diagnostics and minimal residual disease monitoring in infant acute leukemias |
| title_short | Methodological aspects of diagnostics and minimal residual disease monitoring in infant acute leukemias |
| title_sort | methodological aspects of diagnostics and minimal residual disease monitoring in infant acute leukemias |
| topic | 11q23/mll rearrangements infant acute leukemias minimal residual disease |
| url | https://oncohematology.abvpress.ru/ongm/article/view/184 |
| work_keys_str_mv | AT gatsaur methodologicalaspectsofdiagnosticsandminimalresidualdiseasemonitoringininfantacuteleukemias AT ampopov methodologicalaspectsofdiagnosticsandminimalresidualdiseasemonitoringininfantacuteleukemias AT lgfechina methodologicalaspectsofdiagnosticsandminimalresidualdiseasemonitoringininfantacuteleukemias AT sarumyantsev methodologicalaspectsofdiagnosticsandminimalresidualdiseasemonitoringininfantacuteleukemias |