Occult hepatitis B- viral infections among blood donors in Duhok-Iraq
Background & objectives: When a patient has occult hepatitis B virus infection, HBV-DNA is detected in blood or liver tissues with negative HBsAg, with or without anti-HBc antibodies. The study's objective was to ascertain the prevalence of occult HBV infection among Duhok City blood donors...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Kurdistan Higher Council Of Medical Specialties
2025-03-01
|
| Series: | Advanced Medical Journal |
| Subjects: | |
| Online Access: | https://amj.khcms.edu.krd/index.php/main/article/view/589 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850174394422263808 |
|---|---|
| author | Noor Rizqo Mikha Amer Abdalla Goreal |
| author_facet | Noor Rizqo Mikha Amer Abdalla Goreal |
| author_sort | Noor Rizqo Mikha |
| collection | DOAJ |
| description | Background & objectives: When a patient has occult hepatitis B virus infection, HBV-DNA is detected in blood or liver tissues with negative HBsAg, with or without anti-HBc antibodies. The study's objective was to ascertain the prevalence of occult HBV infection among Duhok City blood donors.
Methods: A cross-sectional study has been conducted at Duhok blood bank from November 2022 to January 2023. Plasma samples were obtained from 100 willing blood donors (98male, 2 females, 21-67 years old) who tested negative for HBsAg but positive for AntiHBc. HBsAg negative/anti-HBc positive blood donors have been examined by Anti-HBs II (immunoassay for quantitative detection of antibodies to hepatitis B surface antigen). Next, with the use of a Viral Nucleic Acid Extraction Kit, DNA was isolated from plasma samples. An automated real-time PCR approach was used to identify HBV DNA.
Results: Of the 3156 HBsAg negative donors, 100 (3.16%) tested positive for anti HBc antibodies (total), 20 (20%) tested negative, and 80 (80%) tested positive for anti HBs Antibodies. All 100 donors had negative results from the real-time PCR with no HBV-DNA. No significant correlations were found between AntiHBc and AntiHBs results with sex and different age groups of donors.
Conclusion: No occult HBV infection was detected among Anti-HBc positive donors who were deferral from blood bank reserve. Taking in consideration that 80% of them have protective titer of Anti -HBs, reevaluation of the exemption of such blood donors should be done to reduce the deferral of donors and enhance blood bank reserve. |
| format | Article |
| id | doaj-art-ca3d67c491484c34ac3e699b5b4e25fd |
| institution | OA Journals |
| issn | 2958-8979 2957-3580 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Kurdistan Higher Council Of Medical Specialties |
| record_format | Article |
| series | Advanced Medical Journal |
| spelling | doaj-art-ca3d67c491484c34ac3e699b5b4e25fd2025-08-20T02:19:40ZengKurdistan Higher Council Of Medical SpecialtiesAdvanced Medical Journal2958-89792957-35802025-03-011015965https://doi.org/10.56056/amj.2025.318590Occult hepatitis B- viral infections among blood donors in Duhok-IraqNoor Rizqo Mikha0Amer Abdalla Goreal1M,B,Ch,B., KHCMS Clinical Microbiology student, Azadi Teaching Hospital-DuhokM,B,Ch,B. M.Sc. F.I.B.M.S. Assist. Prof. Department of Medical Microbiology, College of Medicine, University of DuhokBackground & objectives: When a patient has occult hepatitis B virus infection, HBV-DNA is detected in blood or liver tissues with negative HBsAg, with or without anti-HBc antibodies. The study's objective was to ascertain the prevalence of occult HBV infection among Duhok City blood donors. Methods: A cross-sectional study has been conducted at Duhok blood bank from November 2022 to January 2023. Plasma samples were obtained from 100 willing blood donors (98male, 2 females, 21-67 years old) who tested negative for HBsAg but positive for AntiHBc. HBsAg negative/anti-HBc positive blood donors have been examined by Anti-HBs II (immunoassay for quantitative detection of antibodies to hepatitis B surface antigen). Next, with the use of a Viral Nucleic Acid Extraction Kit, DNA was isolated from plasma samples. An automated real-time PCR approach was used to identify HBV DNA. Results: Of the 3156 HBsAg negative donors, 100 (3.16%) tested positive for anti HBc antibodies (total), 20 (20%) tested negative, and 80 (80%) tested positive for anti HBs Antibodies. All 100 donors had negative results from the real-time PCR with no HBV-DNA. No significant correlations were found between AntiHBc and AntiHBs results with sex and different age groups of donors. Conclusion: No occult HBV infection was detected among Anti-HBc positive donors who were deferral from blood bank reserve. Taking in consideration that 80% of them have protective titer of Anti -HBs, reevaluation of the exemption of such blood donors should be done to reduce the deferral of donors and enhance blood bank reserve.https://amj.khcms.edu.krd/index.php/main/article/view/589anti hbcblood donorshepatitis b virusoccult hepatitis b |
| spellingShingle | Noor Rizqo Mikha Amer Abdalla Goreal Occult hepatitis B- viral infections among blood donors in Duhok-Iraq Advanced Medical Journal anti hbc blood donors hepatitis b virus occult hepatitis b |
| title | Occult hepatitis B- viral infections among blood donors in Duhok-Iraq |
| title_full | Occult hepatitis B- viral infections among blood donors in Duhok-Iraq |
| title_fullStr | Occult hepatitis B- viral infections among blood donors in Duhok-Iraq |
| title_full_unstemmed | Occult hepatitis B- viral infections among blood donors in Duhok-Iraq |
| title_short | Occult hepatitis B- viral infections among blood donors in Duhok-Iraq |
| title_sort | occult hepatitis b viral infections among blood donors in duhok iraq |
| topic | anti hbc blood donors hepatitis b virus occult hepatitis b |
| url | https://amj.khcms.edu.krd/index.php/main/article/view/589 |
| work_keys_str_mv | AT noorrizqomikha occulthepatitisbviralinfectionsamongblooddonorsinduhokiraq AT amerabdallagoreal occulthepatitisbviralinfectionsamongblooddonorsinduhokiraq |