Comparative evaluation of free available chlorine in sodium hypochlorite solutions admixed with novel chelating agents

Aim: This study evaluated the effects of ethylenediaminetetraacetic acid (EDTA), etidronic acid (HEBP), peracetic acid (PAA), phytic acid (IP6), and glycolic acid (AHA) on the free available chlorine (FAC) content of sodium hypochlorite (NaOCl) solution when mixed in varying proportions. Methods: Tw...

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Main Authors: Somya Tyagi, Sonali Taneja, Kandasamy Nagarajan, Divya Chowdhary
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2025-04-01
Series:Endodontology
Subjects:
Online Access:https://journals.lww.com/10.4103/endo.endo_219_24
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author Somya Tyagi
Sonali Taneja
Kandasamy Nagarajan
Divya Chowdhary
author_facet Somya Tyagi
Sonali Taneja
Kandasamy Nagarajan
Divya Chowdhary
author_sort Somya Tyagi
collection DOAJ
description Aim: This study evaluated the effects of ethylenediaminetetraacetic acid (EDTA), etidronic acid (HEBP), peracetic acid (PAA), phytic acid (IP6), and glycolic acid (AHA) on the free available chlorine (FAC) content of sodium hypochlorite (NaOCl) solution when mixed in varying proportions. Methods: Twenty-five milliliter aliquots of 5.25% NaOCl were admixed in ratios of 90:10, 80:20, and 50:50 with secondary irrigation solutions: (a) 17% EDTA, (b) 9% HEBP, (c) 1% PAA, (d) 1% IP6, and (e) 10% AHA. Iodometric titration was done to evaluate the change in FAC. Statistical differences between means were determined using a post hoc Tukey’s analysis test after an analysis of variance. Results: On titration, the mean FAC value of NaOCl at baseline was 2.02 g%. On mixing NaOCl with EDTA and HEBP, the FAC decreased in all three proportions. On mixing NaOCl and PAA, the FAC increased on increasing dilution in all three proportions. On mixing NaOCl and IP6, the FAC decreased only in 80:20 dilutions. On mixing NaOCl and AHA, the FAC increased on increasing dilution in all three proportions. Conclusion: The FAC on the combination of NaOCl with AHA, PAA, and IP6 remained unaltered or decreased marginally, suggesting that the antimicrobial properties of NaOCl might be preserved.
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institution Kabale University
issn 0970-7212
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language English
publishDate 2025-04-01
publisher Wolters Kluwer Medknow Publications
record_format Article
series Endodontology
spelling doaj-art-ca3afb2c778345c99257fc5876d9740c2025-08-20T03:29:18ZengWolters Kluwer Medknow PublicationsEndodontology0970-72122543-08312025-04-0137218819310.4103/endo.endo_219_24Comparative evaluation of free available chlorine in sodium hypochlorite solutions admixed with novel chelating agentsSomya TyagiSonali TanejaKandasamy NagarajanDivya ChowdharyAim: This study evaluated the effects of ethylenediaminetetraacetic acid (EDTA), etidronic acid (HEBP), peracetic acid (PAA), phytic acid (IP6), and glycolic acid (AHA) on the free available chlorine (FAC) content of sodium hypochlorite (NaOCl) solution when mixed in varying proportions. Methods: Twenty-five milliliter aliquots of 5.25% NaOCl were admixed in ratios of 90:10, 80:20, and 50:50 with secondary irrigation solutions: (a) 17% EDTA, (b) 9% HEBP, (c) 1% PAA, (d) 1% IP6, and (e) 10% AHA. Iodometric titration was done to evaluate the change in FAC. Statistical differences between means were determined using a post hoc Tukey’s analysis test after an analysis of variance. Results: On titration, the mean FAC value of NaOCl at baseline was 2.02 g%. On mixing NaOCl with EDTA and HEBP, the FAC decreased in all three proportions. On mixing NaOCl and PAA, the FAC increased on increasing dilution in all three proportions. On mixing NaOCl and IP6, the FAC decreased only in 80:20 dilutions. On mixing NaOCl and AHA, the FAC increased on increasing dilution in all three proportions. Conclusion: The FAC on the combination of NaOCl with AHA, PAA, and IP6 remained unaltered or decreased marginally, suggesting that the antimicrobial properties of NaOCl might be preserved.https://journals.lww.com/10.4103/endo.endo_219_24etidronic acidfree available chlorineglycolic acidperacetic acidphytic acidsodium hypochlorite
spellingShingle Somya Tyagi
Sonali Taneja
Kandasamy Nagarajan
Divya Chowdhary
Comparative evaluation of free available chlorine in sodium hypochlorite solutions admixed with novel chelating agents
Endodontology
etidronic acid
free available chlorine
glycolic acid
peracetic acid
phytic acid
sodium hypochlorite
title Comparative evaluation of free available chlorine in sodium hypochlorite solutions admixed with novel chelating agents
title_full Comparative evaluation of free available chlorine in sodium hypochlorite solutions admixed with novel chelating agents
title_fullStr Comparative evaluation of free available chlorine in sodium hypochlorite solutions admixed with novel chelating agents
title_full_unstemmed Comparative evaluation of free available chlorine in sodium hypochlorite solutions admixed with novel chelating agents
title_short Comparative evaluation of free available chlorine in sodium hypochlorite solutions admixed with novel chelating agents
title_sort comparative evaluation of free available chlorine in sodium hypochlorite solutions admixed with novel chelating agents
topic etidronic acid
free available chlorine
glycolic acid
peracetic acid
phytic acid
sodium hypochlorite
url https://journals.lww.com/10.4103/endo.endo_219_24
work_keys_str_mv AT somyatyagi comparativeevaluationoffreeavailablechlorineinsodiumhypochloritesolutionsadmixedwithnovelchelatingagents
AT sonalitaneja comparativeevaluationoffreeavailablechlorineinsodiumhypochloritesolutionsadmixedwithnovelchelatingagents
AT kandasamynagarajan comparativeevaluationoffreeavailablechlorineinsodiumhypochloritesolutionsadmixedwithnovelchelatingagents
AT divyachowdhary comparativeevaluationoffreeavailablechlorineinsodiumhypochloritesolutionsadmixedwithnovelchelatingagents