Post-Translational Modification of p62: Roles and Regulations in Autophagy

Autophagy is a highly conserved cellular process that plays a crucial role in maintaining cellular homeostasis by degrading damaged organelles, misfolded proteins, and other cellular components. p62/SQSTM1 functions as a selective autophagy receptor by binding polyubiquitinated cargo through its UBA...

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Main Authors: Shuai Xiao, Yeping Yu, Meng Liao, Dandan Song, Xiaozhen Xu, Lingli Tian, Rui Zhang, Hao Lyu, Dong Guo, Qi Zhang, Xing-Zhen Chen, Cefan Zhou, Jingfeng Tang
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Cells
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Online Access:https://www.mdpi.com/2073-4409/14/13/1016
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author Shuai Xiao
Yeping Yu
Meng Liao
Dandan Song
Xiaozhen Xu
Lingli Tian
Rui Zhang
Hao Lyu
Dong Guo
Qi Zhang
Xing-Zhen Chen
Cefan Zhou
Jingfeng Tang
author_facet Shuai Xiao
Yeping Yu
Meng Liao
Dandan Song
Xiaozhen Xu
Lingli Tian
Rui Zhang
Hao Lyu
Dong Guo
Qi Zhang
Xing-Zhen Chen
Cefan Zhou
Jingfeng Tang
author_sort Shuai Xiao
collection DOAJ
description Autophagy is a highly conserved cellular process that plays a crucial role in maintaining cellular homeostasis by degrading damaged organelles, misfolded proteins, and other cellular components. p62/SQSTM1 functions as a selective autophagy receptor by binding polyubiquitinated cargo through its UBA domain and linking it to microtubule-associated protein light chain 3 (LC3)-decorated autophagosomes. Moreover, p62 acts as a signaling hub and is essential in response to various stressors, including nutrient deprivation and oxidative stress. Post-translational modifications (PTMs) critically regulate p62’s multifaceted roles, controlling p62’s phase separation, cargo recruitment, signaling interactions, and autophagic degradation efficiency. The dysregulation of p62 PTMs is closely related to the occurrence and development of human diseases, particularly neurodegenerative disorders and certain cancers. This review summarizes the main PTM events of p62 discovered to date that influence the autophagy process, including phosphorylation, acetylation, ubiquitination, and S-acylation, as well as their known contributions to protein aggregation and disease. The PTMs of p62 dynamically regulate autophagy, protein aggregation, and cellular signaling, underscoring its importance as a potential therapeutic target and biomarker for these diseases.
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spelling doaj-art-ca3a802232884de9a7bfb3e2c9be7f9e2025-08-20T03:16:55ZengMDPI AGCells2073-44092025-07-011413101610.3390/cells14131016Post-Translational Modification of p62: Roles and Regulations in AutophagyShuai Xiao0Yeping Yu1Meng Liao2Dandan Song3Xiaozhen Xu4Lingli Tian5Rui Zhang6Hao Lyu7Dong Guo8Qi Zhang9Xing-Zhen Chen10Cefan Zhou11Jingfeng Tang12National “111” Center for Cellular Regulation and Molecular Pharmaceutics, Hubei University of Technology, Wuhan 430068, ChinaNational “111” Center for Cellular Regulation and Molecular Pharmaceutics, Hubei University of Technology, Wuhan 430068, ChinaNational “111” Center for Cellular Regulation and Molecular Pharmaceutics, Hubei University of Technology, Wuhan 430068, ChinaNational “111” Center for Cellular Regulation and Molecular Pharmaceutics, Hubei University of Technology, Wuhan 430068, ChinaNational “111” Center for Cellular Regulation and Molecular Pharmaceutics, Hubei University of Technology, Wuhan 430068, ChinaNational “111” Center for Cellular Regulation and Molecular Pharmaceutics, Hubei University of Technology, Wuhan 430068, ChinaNational “111” Center for Cellular Regulation and Molecular Pharmaceutics, Hubei University of Technology, Wuhan 430068, ChinaNational “111” Center for Cellular Regulation and Molecular Pharmaceutics, Hubei University of Technology, Wuhan 430068, ChinaNational “111” Center for Cellular Regulation and Molecular Pharmaceutics, Hubei University of Technology, Wuhan 430068, ChinaNational “111” Center for Cellular Regulation and Molecular Pharmaceutics, Hubei University of Technology, Wuhan 430068, ChinaMembrane Protein Disease Research Group, Department of Physiology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB T6G 2R3, CanadaNational “111” Center for Cellular Regulation and Molecular Pharmaceutics, Hubei University of Technology, Wuhan 430068, ChinaNational “111” Center for Cellular Regulation and Molecular Pharmaceutics, Hubei University of Technology, Wuhan 430068, ChinaAutophagy is a highly conserved cellular process that plays a crucial role in maintaining cellular homeostasis by degrading damaged organelles, misfolded proteins, and other cellular components. p62/SQSTM1 functions as a selective autophagy receptor by binding polyubiquitinated cargo through its UBA domain and linking it to microtubule-associated protein light chain 3 (LC3)-decorated autophagosomes. Moreover, p62 acts as a signaling hub and is essential in response to various stressors, including nutrient deprivation and oxidative stress. Post-translational modifications (PTMs) critically regulate p62’s multifaceted roles, controlling p62’s phase separation, cargo recruitment, signaling interactions, and autophagic degradation efficiency. The dysregulation of p62 PTMs is closely related to the occurrence and development of human diseases, particularly neurodegenerative disorders and certain cancers. This review summarizes the main PTM events of p62 discovered to date that influence the autophagy process, including phosphorylation, acetylation, ubiquitination, and S-acylation, as well as their known contributions to protein aggregation and disease. The PTMs of p62 dynamically regulate autophagy, protein aggregation, and cellular signaling, underscoring its importance as a potential therapeutic target and biomarker for these diseases.https://www.mdpi.com/2073-4409/14/13/1016post-translational modificationsautophagyp62oligomerization
spellingShingle Shuai Xiao
Yeping Yu
Meng Liao
Dandan Song
Xiaozhen Xu
Lingli Tian
Rui Zhang
Hao Lyu
Dong Guo
Qi Zhang
Xing-Zhen Chen
Cefan Zhou
Jingfeng Tang
Post-Translational Modification of p62: Roles and Regulations in Autophagy
Cells
post-translational modifications
autophagy
p62
oligomerization
title Post-Translational Modification of p62: Roles and Regulations in Autophagy
title_full Post-Translational Modification of p62: Roles and Regulations in Autophagy
title_fullStr Post-Translational Modification of p62: Roles and Regulations in Autophagy
title_full_unstemmed Post-Translational Modification of p62: Roles and Regulations in Autophagy
title_short Post-Translational Modification of p62: Roles and Regulations in Autophagy
title_sort post translational modification of p62 roles and regulations in autophagy
topic post-translational modifications
autophagy
p62
oligomerization
url https://www.mdpi.com/2073-4409/14/13/1016
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