Cloning and expression of the receptor gene (Bt-r<sub>3</sub>) of the Bt toxic protein CrylAb in E. coli
An extracellular part of the receptor gene (Bt-r<sub>3</sub>) of the Bt toxic protein Cry1Ab, with a length of about 4500 bp, was amplified by PCR and cloned into plasmid prcHis-TOPO. The recombinant plasmid pTrcHis-TOPO/peBt-r<sub>3</sub> was transformed to E. coli, and expr...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2004-05-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2004.03.0323 |
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| Summary: | An extracellular part of the receptor gene (Bt-r<sub>3</sub>) of the Bt toxic protein Cry1Ab, with a length of about 4500 bp, was amplified by PCR and cloned into plasmid prcHis-TOPO. The recombinant plasmid pTrcHis-TOPO/peBt-r<sub>3</sub> was transformed to E. coli, and expressed at 37℃ by IPTG induction. SDS-PAGE proved that the molecular weight of expressed product was about 160 kD. Western blotting showed that the recombinant protein could specifically bind to Bt toxic protein Cry1Ab, which validated that the Bt-R<sub>3</sub> was a receptor protein of CrylAb. The binding site of Bt-R<sub>3</sub> with CrylAb was located in extracellular domains of Bt-R<sub>3</sub> protein. |
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| ISSN: | 1008-9209 2097-5155 |