A platform for Bioengineering Tissue Membranes from cell spheroids
Cell spheroids are essential building blocks for engineering tissues like cartilage, bone, liver, cardiac, pancreatic, and neural tissues, but controlling their fusion and organisation is challenging. Spheroids tend to fuse into a larger mass, impeding nutrient and waste diffusion. To overcome this,...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-04-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006425000845 |
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| author | Quang Bach Le Hariharan Ezhilarasu Weng Wan Chan Alok Tanala Patra Priya Murugan Shashaank Abhinav Venkatesh Yean Kai Tay Shin Ru Lim Ahmad Amirul Abdul Rahim Jia Sheng Zach Lee Xuezhi Bi Deepak Choudhury |
| author_facet | Quang Bach Le Hariharan Ezhilarasu Weng Wan Chan Alok Tanala Patra Priya Murugan Shashaank Abhinav Venkatesh Yean Kai Tay Shin Ru Lim Ahmad Amirul Abdul Rahim Jia Sheng Zach Lee Xuezhi Bi Deepak Choudhury |
| author_sort | Quang Bach Le |
| collection | DOAJ |
| description | Cell spheroids are essential building blocks for engineering tissues like cartilage, bone, liver, cardiac, pancreatic, and neural tissues, but controlling their fusion and organisation is challenging. Spheroids tend to fuse into a larger mass, impeding nutrient and waste diffusion. To overcome this, we developed a method to assemble spheroids into a thin layer by using two mesh scaffolds to spread them evenly, and a solid frame with grid to secure the assembly. This allows the spheroids to fuse into a thin membrane-like tissue, allowing better medium diffusion during cell culture. We demonstrated this method by producing cartilage tissue membranes from human mesenchymal stem cell spheroids undergoing chondrogenic differentiation, evaluating spheroid sizes, assembly timing, fusion process and membrane thickness. Our method is a versatile platform for producing tissue membranes from cell spheroids, with significant potential in tissue engineering for creating functional tissue constructs from various cell types. |
| format | Article |
| id | doaj-art-ca2d96386e39407b9b2f81b64b1e0948 |
| institution | Kabale University |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-ca2d96386e39407b9b2f81b64b1e09482025-02-12T05:31:41ZengElsevierMaterials Today Bio2590-00642025-04-0131101526A platform for Bioengineering Tissue Membranes from cell spheroidsQuang Bach Le0Hariharan Ezhilarasu1Weng Wan Chan2Alok Tanala Patra3Priya Murugan4Shashaank Abhinav Venkatesh5Yean Kai Tay6Shin Ru Lim7Ahmad Amirul Abdul Rahim8Jia Sheng Zach Lee9Xuezhi Bi10Deepak Choudhury11Biomanufacturing Technology (BMT), Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A∗STAR), 20 Biopolis Way, 138668, Singapore; Corresponding author.Biomanufacturing Technology (BMT), Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A∗STAR), 20 Biopolis Way, 138668, SingaporeBiomanufacturing Technology (BMT), Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A∗STAR), 20 Biopolis Way, 138668, SingaporeBiomanufacturing Technology (BMT), Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A∗STAR), 20 Biopolis Way, 138668, SingaporeBiomanufacturing Technology (BMT), Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A∗STAR), 20 Biopolis Way, 138668, SingaporeBiomanufacturing Technology (BMT), Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A∗STAR), 20 Biopolis Way, 138668, SingaporeBiomanufacturing Technology (BMT), Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A∗STAR), 20 Biopolis Way, 138668, SingaporeBiomanufacturing Technology (BMT), Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A∗STAR), 20 Biopolis Way, 138668, SingaporeBiomanufacturing Technology (BMT), Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A∗STAR), 20 Biopolis Way, 138668, SingaporeBiomanufacturing Technology (BMT), Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A∗STAR), 20 Biopolis Way, 138668, SingaporeBiomanufacturing Technology (BMT), Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A∗STAR), 20 Biopolis Way, 138668, SingaporeBiomanufacturing Technology (BMT), Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A∗STAR), 20 Biopolis Way, 138668, Singapore; Corresponding author. Biomanufacturing Technology (BMT), Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A∗STAR), 20 Biopolis Way, 138668, Singapore.Cell spheroids are essential building blocks for engineering tissues like cartilage, bone, liver, cardiac, pancreatic, and neural tissues, but controlling their fusion and organisation is challenging. Spheroids tend to fuse into a larger mass, impeding nutrient and waste diffusion. To overcome this, we developed a method to assemble spheroids into a thin layer by using two mesh scaffolds to spread them evenly, and a solid frame with grid to secure the assembly. This allows the spheroids to fuse into a thin membrane-like tissue, allowing better medium diffusion during cell culture. We demonstrated this method by producing cartilage tissue membranes from human mesenchymal stem cell spheroids undergoing chondrogenic differentiation, evaluating spheroid sizes, assembly timing, fusion process and membrane thickness. Our method is a versatile platform for producing tissue membranes from cell spheroids, with significant potential in tissue engineering for creating functional tissue constructs from various cell types.http://www.sciencedirect.com/science/article/pii/S2590006425000845BiomanufacturingCell therapyTissue therapyTissue engineeringBioengineeringTissue membrane |
| spellingShingle | Quang Bach Le Hariharan Ezhilarasu Weng Wan Chan Alok Tanala Patra Priya Murugan Shashaank Abhinav Venkatesh Yean Kai Tay Shin Ru Lim Ahmad Amirul Abdul Rahim Jia Sheng Zach Lee Xuezhi Bi Deepak Choudhury A platform for Bioengineering Tissue Membranes from cell spheroids Materials Today Bio Biomanufacturing Cell therapy Tissue therapy Tissue engineering Bioengineering Tissue membrane |
| title | A platform for Bioengineering Tissue Membranes from cell spheroids |
| title_full | A platform for Bioengineering Tissue Membranes from cell spheroids |
| title_fullStr | A platform for Bioengineering Tissue Membranes from cell spheroids |
| title_full_unstemmed | A platform for Bioengineering Tissue Membranes from cell spheroids |
| title_short | A platform for Bioengineering Tissue Membranes from cell spheroids |
| title_sort | platform for bioengineering tissue membranes from cell spheroids |
| topic | Biomanufacturing Cell therapy Tissue therapy Tissue engineering Bioengineering Tissue membrane |
| url | http://www.sciencedirect.com/science/article/pii/S2590006425000845 |
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