Exploring Morphology of Thermoplasmonic Nanoparticles to Synergize Immunotherapeutic Fibroblast Activation Protein‐Positive Cell Sensitization and Photothermal Therapy
The precision of photothermal therapy (PTT) is often hindered by the challenge of achieving selective delivery of thermoplasmonic nanostructures to tumors. Active targeting, which leverages synthetic molecular complexes to address receptors overexpressed by malignant cells, enables such specificity...
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Wiley-VCH
2025-08-01
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| Series: | Small Science |
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| Online Access: | https://doi.org/10.1002/smsc.202500099 |
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| author | Ahmed Alsadig Xuan Peng Hugo Boutier Liliana R. Loureiro Anja Feldmann René Hübner Humberto Cabrera Manja Kubeil Michael Bachmann Larysa Baraban |
| author_facet | Ahmed Alsadig Xuan Peng Hugo Boutier Liliana R. Loureiro Anja Feldmann René Hübner Humberto Cabrera Manja Kubeil Michael Bachmann Larysa Baraban |
| author_sort | Ahmed Alsadig |
| collection | DOAJ |
| description | The precision of photothermal therapy (PTT) is often hindered by the challenge of achieving selective delivery of thermoplasmonic nanostructures to tumors. Active targeting, which leverages synthetic molecular complexes to address receptors overexpressed by malignant cells, enables such specificity and facilitates the combination of the PTT with other anticancer therapies. In this study, we developed thermoplasmonic nanoconjugates consisting of (i) 20 nm spherical gold nanoparticles (AuNPs) or gold nanostars (AuNSs) as nanocarriers, and (ii) surface‐passivated antibody‐based fibroblast activation protein (FAP)‐targeting modules, used in adaptive chimeric antigen receptor T‐cells immunotherapy. The nanoconjugates demonstrated excellent stability and specific binding to FAP‐expressing fibrosarcoma HT1080 genetically modified to express human FAP, as confirmed by fluorescence activated cell sorting, immunofluorescence, and surface plasmon resonance scattering imaging. Moreover, the nanocarriers showed significant photothermal conversion after visible and near‐infrared irradiation. Quantitative thermal lens spectroscopy demonstrated the superior photothermal capability of AuNSs, achieving up to 1.5‐fold greater thermal enhancement than AuNPs under identical conditions. This synergistic approach, combining targeted immunotherapy with the thermoplasmonic nanocarriers, not only streamlines nanoparticle delivery, increasing photothermal yield and therapeutic efficacy but also offers a comprehensive and potent strategy for cancer treatment with the potential for superior outcomes across multiple modalities. |
| format | Article |
| id | doaj-art-ca2b3d5bed2a402296651af3349d505b |
| institution | DOAJ |
| issn | 2688-4046 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Small Science |
| spelling | doaj-art-ca2b3d5bed2a402296651af3349d505b2025-08-20T03:04:01ZengWiley-VCHSmall Science2688-40462025-08-0158n/an/a10.1002/smsc.202500099Exploring Morphology of Thermoplasmonic Nanoparticles to Synergize Immunotherapeutic Fibroblast Activation Protein‐Positive Cell Sensitization and Photothermal TherapyAhmed Alsadig0Xuan Peng1Hugo Boutier2Liliana R. Loureiro3Anja Feldmann4René Hübner5Humberto Cabrera6Manja Kubeil7Michael Bachmann8Larysa Baraban9Institute of Radiopharmaceutical Cancer Research Helmholtz‐Zentrum Dresden‐Rossendorf e. V. 01328 Dresden GermanyInstitute of Radiopharmaceutical Cancer Research Helmholtz‐Zentrum Dresden‐Rossendorf e. V. 01328 Dresden GermanyInstitute of Radiopharmaceutical Cancer Research Helmholtz‐Zentrum Dresden‐Rossendorf e. V. 01328 Dresden GermanyInstitute of Radiopharmaceutical Cancer Research Helmholtz‐Zentrum Dresden‐Rossendorf e. V. 01328 Dresden GermanyInstitute of Radiopharmaceutical Cancer Research Helmholtz‐Zentrum Dresden‐Rossendorf e. V. 01328 Dresden GermanyInstitute of Ion Beam Physics and Materials Research Helmholtz‐Zenztrum Dresden‐Rossendorf e. V. 01328 Dresden GermanyThe Abdus Salam International Centre for Theoretical Physics MLab STI Unit 34151 Trieste ItalyInstitute of Radiopharmaceutical Cancer Research Helmholtz‐Zentrum Dresden‐Rossendorf e. V. 01328 Dresden GermanyInstitute of Radiopharmaceutical Cancer Research Helmholtz‐Zentrum Dresden‐Rossendorf e. V. 01328 Dresden GermanyInstitute of Radiopharmaceutical Cancer Research Helmholtz‐Zentrum Dresden‐Rossendorf e. V. 01328 Dresden GermanyThe precision of photothermal therapy (PTT) is often hindered by the challenge of achieving selective delivery of thermoplasmonic nanostructures to tumors. Active targeting, which leverages synthetic molecular complexes to address receptors overexpressed by malignant cells, enables such specificity and facilitates the combination of the PTT with other anticancer therapies. In this study, we developed thermoplasmonic nanoconjugates consisting of (i) 20 nm spherical gold nanoparticles (AuNPs) or gold nanostars (AuNSs) as nanocarriers, and (ii) surface‐passivated antibody‐based fibroblast activation protein (FAP)‐targeting modules, used in adaptive chimeric antigen receptor T‐cells immunotherapy. The nanoconjugates demonstrated excellent stability and specific binding to FAP‐expressing fibrosarcoma HT1080 genetically modified to express human FAP, as confirmed by fluorescence activated cell sorting, immunofluorescence, and surface plasmon resonance scattering imaging. Moreover, the nanocarriers showed significant photothermal conversion after visible and near‐infrared irradiation. Quantitative thermal lens spectroscopy demonstrated the superior photothermal capability of AuNSs, achieving up to 1.5‐fold greater thermal enhancement than AuNPs under identical conditions. This synergistic approach, combining targeted immunotherapy with the thermoplasmonic nanocarriers, not only streamlines nanoparticle delivery, increasing photothermal yield and therapeutic efficacy but also offers a comprehensive and potent strategy for cancer treatment with the potential for superior outcomes across multiple modalities.https://doi.org/10.1002/smsc.202500099fibroblast activation proteingold nanoparticlesimmunotherapeutic target modulesphotothermal therapyspecific cell targetingthermal lens spectroscopy |
| spellingShingle | Ahmed Alsadig Xuan Peng Hugo Boutier Liliana R. Loureiro Anja Feldmann René Hübner Humberto Cabrera Manja Kubeil Michael Bachmann Larysa Baraban Exploring Morphology of Thermoplasmonic Nanoparticles to Synergize Immunotherapeutic Fibroblast Activation Protein‐Positive Cell Sensitization and Photothermal Therapy Small Science fibroblast activation protein gold nanoparticles immunotherapeutic target modules photothermal therapy specific cell targeting thermal lens spectroscopy |
| title | Exploring Morphology of Thermoplasmonic Nanoparticles to Synergize Immunotherapeutic Fibroblast Activation Protein‐Positive Cell Sensitization and Photothermal Therapy |
| title_full | Exploring Morphology of Thermoplasmonic Nanoparticles to Synergize Immunotherapeutic Fibroblast Activation Protein‐Positive Cell Sensitization and Photothermal Therapy |
| title_fullStr | Exploring Morphology of Thermoplasmonic Nanoparticles to Synergize Immunotherapeutic Fibroblast Activation Protein‐Positive Cell Sensitization and Photothermal Therapy |
| title_full_unstemmed | Exploring Morphology of Thermoplasmonic Nanoparticles to Synergize Immunotherapeutic Fibroblast Activation Protein‐Positive Cell Sensitization and Photothermal Therapy |
| title_short | Exploring Morphology of Thermoplasmonic Nanoparticles to Synergize Immunotherapeutic Fibroblast Activation Protein‐Positive Cell Sensitization and Photothermal Therapy |
| title_sort | exploring morphology of thermoplasmonic nanoparticles to synergize immunotherapeutic fibroblast activation protein positive cell sensitization and photothermal therapy |
| topic | fibroblast activation protein gold nanoparticles immunotherapeutic target modules photothermal therapy specific cell targeting thermal lens spectroscopy |
| url | https://doi.org/10.1002/smsc.202500099 |
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