FANCI is involved in the malignant progression of glioma cells by regulating the Akt/Bcl-2 signaling pathway
Abstract Introduction Fanconi anemia supplementation group I (FANCI), one of the Fanconi family proteins, may be closely related to the malignant progression of glioma cells. Here, we sought to validate the expression of FANCI in glioma cells and its role in regulating the Akt signaling pathway in t...
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| Format: | Article |
| Language: | English |
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Springer
2025-05-01
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| Series: | Discover Oncology |
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| Online Access: | https://doi.org/10.1007/s12672-025-02284-x |
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| author | Hanyun Li Yinrui Liu Mingyao Wang Shaoshao Xu Jilan Liu Xianyun Qin Banban Li Jikui Sun Yan Liu Feng Jin |
| author_facet | Hanyun Li Yinrui Liu Mingyao Wang Shaoshao Xu Jilan Liu Xianyun Qin Banban Li Jikui Sun Yan Liu Feng Jin |
| author_sort | Hanyun Li |
| collection | DOAJ |
| description | Abstract Introduction Fanconi anemia supplementation group I (FANCI), one of the Fanconi family proteins, may be closely related to the malignant progression of glioma cells. Here, we sought to validate the expression of FANCI in glioma cells and its role in regulating the Akt signaling pathway in the malignant progression of glioma cells. Methods The expression of FANCI in glioma cells was analyzed by bioinformatics and microarray. Lentivirus transfection was used to regulate the expression of FANCI in glioma cells. CCK-8, EdU, Transwell, and flow cytometry were used to observe the effect of FANCI on the malignant progression of glioma cells. Results We found that low expression of FANCI inhibited the proliferation, migration and invasion of human glioma cells, and promoted cell apoptosis. However, overexpression of FANCI produced the opposite effect. We also found that low expression of FANCI inhibited the expression of P-Akt and B-cell lymphoma-2 (Bcl-2). Finally, we validated these in vitro results in a xenograft mouse model. Conclusion FANCI can inhibit the development of glioma by inhibiting Akt/Bcl-2 signaling pathway. |
| format | Article |
| id | doaj-art-ca25968dba2f439ba89909cdbec5ba74 |
| institution | OA Journals |
| issn | 2730-6011 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Springer |
| record_format | Article |
| series | Discover Oncology |
| spelling | doaj-art-ca25968dba2f439ba89909cdbec5ba742025-08-20T01:51:36ZengSpringerDiscover Oncology2730-60112025-05-0116111610.1007/s12672-025-02284-xFANCI is involved in the malignant progression of glioma cells by regulating the Akt/Bcl-2 signaling pathwayHanyun Li0Yinrui Liu1Mingyao Wang2Shaoshao Xu3Jilan Liu4Xianyun Qin5Banban Li6Jikui Sun7Yan Liu8Feng Jin9Cheeloo College of Medicine, Shandong UniversityDepartment of Neurosurgery, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan HospitalJining Medical UniversityCheeloo College of Medicine, Shandong UniversityDepartment of Medical Research Center, Affiliated Hospital of Jining Medical UniversityDepartment of Medical Research Center, Affiliated Hospital of Jining Medical UniversityDepartment of Hematology, The Affiliated Taian City Central Hospital of Qingdao UniversityDepartment of Neurosurgery, Huanhu Hospital, Tianjin Medical UniversitySchool of Public Health, Jining Medical UniversityDepartment of Neurosurgery, Qingdao Central Hospital, University of Health and Rehabilitation SciencesAbstract Introduction Fanconi anemia supplementation group I (FANCI), one of the Fanconi family proteins, may be closely related to the malignant progression of glioma cells. Here, we sought to validate the expression of FANCI in glioma cells and its role in regulating the Akt signaling pathway in the malignant progression of glioma cells. Methods The expression of FANCI in glioma cells was analyzed by bioinformatics and microarray. Lentivirus transfection was used to regulate the expression of FANCI in glioma cells. CCK-8, EdU, Transwell, and flow cytometry were used to observe the effect of FANCI on the malignant progression of glioma cells. Results We found that low expression of FANCI inhibited the proliferation, migration and invasion of human glioma cells, and promoted cell apoptosis. However, overexpression of FANCI produced the opposite effect. We also found that low expression of FANCI inhibited the expression of P-Akt and B-cell lymphoma-2 (Bcl-2). Finally, we validated these in vitro results in a xenograft mouse model. Conclusion FANCI can inhibit the development of glioma by inhibiting Akt/Bcl-2 signaling pathway.https://doi.org/10.1007/s12672-025-02284-xGliomaFANCIAkt/Bcl-2ProliferationApoptosis |
| spellingShingle | Hanyun Li Yinrui Liu Mingyao Wang Shaoshao Xu Jilan Liu Xianyun Qin Banban Li Jikui Sun Yan Liu Feng Jin FANCI is involved in the malignant progression of glioma cells by regulating the Akt/Bcl-2 signaling pathway Discover Oncology Glioma FANCI Akt/Bcl-2 Proliferation Apoptosis |
| title | FANCI is involved in the malignant progression of glioma cells by regulating the Akt/Bcl-2 signaling pathway |
| title_full | FANCI is involved in the malignant progression of glioma cells by regulating the Akt/Bcl-2 signaling pathway |
| title_fullStr | FANCI is involved in the malignant progression of glioma cells by regulating the Akt/Bcl-2 signaling pathway |
| title_full_unstemmed | FANCI is involved in the malignant progression of glioma cells by regulating the Akt/Bcl-2 signaling pathway |
| title_short | FANCI is involved in the malignant progression of glioma cells by regulating the Akt/Bcl-2 signaling pathway |
| title_sort | fanci is involved in the malignant progression of glioma cells by regulating the akt bcl 2 signaling pathway |
| topic | Glioma FANCI Akt/Bcl-2 Proliferation Apoptosis |
| url | https://doi.org/10.1007/s12672-025-02284-x |
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