Biochemical and gene expression profiling of five pear species under drought stress conditions
Abstract Drought is one of the crucial abiotic stresses which affects growth, development, and performance of pear trees. This research was performed to investigate responses of five pear species including Pyrus communis L., Pyrus boissieriana Bushe., Pyrus glabra Boiss., Pyrus syriaca Boiss., and P...
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| Language: | English |
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BMC
2025-03-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-025-06408-x |
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| author | Lavin Babaei M. Mehdi Sharifani Reza Darvishzadeh Naser Abbaspour Mashhid Henareh |
| author_facet | Lavin Babaei M. Mehdi Sharifani Reza Darvishzadeh Naser Abbaspour Mashhid Henareh |
| author_sort | Lavin Babaei |
| collection | DOAJ |
| description | Abstract Drought is one of the crucial abiotic stresses which affects growth, development, and performance of pear trees. This research was performed to investigate responses of five pear species including Pyrus communis L., Pyrus boissieriana Bushe., Pyrus glabra Boiss., Pyrus syriaca Boiss., and Pyrus salicifolia Pall. to different levels of drought stress. The potted trees were irrigated with water volume of 100%, 60%, or 30% of field capacity (FC) during 90 days. Based on the visual observation, the plant growth was restricted by severe drought in all species. Malondialdehyde (MDA) and glycine betaine (GB) contents, as well as the ascorbate peroxidase (APX), guaiacol peroxidase (GPX) and catalase (CAT) activities were indicated an uprising behavior under drought stress. Principal component analysis (PCA) analysis revealed P. glabra as tolerant and P. communis as sensitive to drought stress. The expression rates of stress-responsive transcription factors (TFs); WRKY29 and DREB6 and their responding genes, including LEA29 and Dehydrin1, were analyzed in the two differentially drought-responding pear species i.e., P. glabra and P. communis. The expression of the studied TFs was induced when both species were exposed to higher drought levels. The species P. glabra exhibited better osmoregulation, antioxidant response, and higher up-regulation of WRKY29, DREB6, LEA29 and Dehydrin1. In conclusion, among the studied pear species, P. glabra could best tolerate drought stress by boosting protective mechanisms. |
| format | Article |
| id | doaj-art-ca1d4eff9b074d3ab219dc599b03874d |
| institution | Kabale University |
| issn | 1471-2229 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj-art-ca1d4eff9b074d3ab219dc599b03874d2025-08-20T03:40:45ZengBMCBMC Plant Biology1471-22292025-03-0125111310.1186/s12870-025-06408-xBiochemical and gene expression profiling of five pear species under drought stress conditionsLavin Babaei0M. Mehdi Sharifani1Reza Darvishzadeh2Naser Abbaspour3Mashhid Henareh4Department of Horticulture, Gorgan University of Agricultural Sciences and Natural ResourcesDepartment of Horticulture, Gorgan University of Agricultural Sciences and Natural ResourcesDepartment of Plant Production and Genetics, Faculty of Agriculture, Urmia UniversityDepartment of Biology, Faculty of Sciences, Urmia UniversitySeed and Plant Improvement Research Department, West Azarbaijan Agricultural and Natural Resources Research and Education Center, AREEOAbstract Drought is one of the crucial abiotic stresses which affects growth, development, and performance of pear trees. This research was performed to investigate responses of five pear species including Pyrus communis L., Pyrus boissieriana Bushe., Pyrus glabra Boiss., Pyrus syriaca Boiss., and Pyrus salicifolia Pall. to different levels of drought stress. The potted trees were irrigated with water volume of 100%, 60%, or 30% of field capacity (FC) during 90 days. Based on the visual observation, the plant growth was restricted by severe drought in all species. Malondialdehyde (MDA) and glycine betaine (GB) contents, as well as the ascorbate peroxidase (APX), guaiacol peroxidase (GPX) and catalase (CAT) activities were indicated an uprising behavior under drought stress. Principal component analysis (PCA) analysis revealed P. glabra as tolerant and P. communis as sensitive to drought stress. The expression rates of stress-responsive transcription factors (TFs); WRKY29 and DREB6 and their responding genes, including LEA29 and Dehydrin1, were analyzed in the two differentially drought-responding pear species i.e., P. glabra and P. communis. The expression of the studied TFs was induced when both species were exposed to higher drought levels. The species P. glabra exhibited better osmoregulation, antioxidant response, and higher up-regulation of WRKY29, DREB6, LEA29 and Dehydrin1. In conclusion, among the studied pear species, P. glabra could best tolerate drought stress by boosting protective mechanisms.https://doi.org/10.1186/s12870-025-06408-xAntioxidant enzymesPyrus spp.Stress-responsive genesWater deficit conditions |
| spellingShingle | Lavin Babaei M. Mehdi Sharifani Reza Darvishzadeh Naser Abbaspour Mashhid Henareh Biochemical and gene expression profiling of five pear species under drought stress conditions BMC Plant Biology Antioxidant enzymes Pyrus spp. Stress-responsive genes Water deficit conditions |
| title | Biochemical and gene expression profiling of five pear species under drought stress conditions |
| title_full | Biochemical and gene expression profiling of five pear species under drought stress conditions |
| title_fullStr | Biochemical and gene expression profiling of five pear species under drought stress conditions |
| title_full_unstemmed | Biochemical and gene expression profiling of five pear species under drought stress conditions |
| title_short | Biochemical and gene expression profiling of five pear species under drought stress conditions |
| title_sort | biochemical and gene expression profiling of five pear species under drought stress conditions |
| topic | Antioxidant enzymes Pyrus spp. Stress-responsive genes Water deficit conditions |
| url | https://doi.org/10.1186/s12870-025-06408-x |
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