Protocol for inducing epidermis-specific gene deletion and establishing a UVB-irradiated skin inflammation model
Summary: When skin is exposed to ultraviolet B (UVB) radiation, damaged keratinocytes release double-stranded RNA to trigger an inflammatory response. Here, we present a protocol to induce epidermal-specific Zfp750 gene-deficient mice and establish a UVB-induced skin inflammation model. We detail pr...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725001078 |
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| author | Ye Liu George L. Sen |
| author_facet | Ye Liu George L. Sen |
| author_sort | Ye Liu |
| collection | DOAJ |
| description | Summary: When skin is exposed to ultraviolet B (UVB) radiation, damaged keratinocytes release double-stranded RNA to trigger an inflammatory response. Here, we present a protocol to induce epidermal-specific Zfp750 gene-deficient mice and establish a UVB-induced skin inflammation model. We detail procedures for detecting gene expressions in the epidermis by quantitative reverse-transcription PCR (RT-qPCR) and analyzing changes in skin immune cell subsets after UVB radiation using flow cytometry.For complete details on the use and execution of this protocol, please refer to Liu et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-ca1ba12d89d94ce3848c774ffa7d801f |
| institution | DOAJ |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-ca1ba12d89d94ce3848c774ffa7d801f2025-08-20T02:55:54ZengElsevierSTAR Protocols2666-16672025-06-016210370110.1016/j.xpro.2025.103701Protocol for inducing epidermis-specific gene deletion and establishing a UVB-irradiated skin inflammation modelYe Liu0George L. Sen1Department of Dermatology, Department of Cellular and Molecular Medicine, Division of Epithelial Biology, University of California, San Diego, La Jolla, CA 92093-0869, USA; Corresponding authorDepartment of Dermatology, Department of Cellular and Molecular Medicine, Division of Epithelial Biology, University of California, San Diego, La Jolla, CA 92093-0869, USA; Corresponding authorSummary: When skin is exposed to ultraviolet B (UVB) radiation, damaged keratinocytes release double-stranded RNA to trigger an inflammatory response. Here, we present a protocol to induce epidermal-specific Zfp750 gene-deficient mice and establish a UVB-induced skin inflammation model. We detail procedures for detecting gene expressions in the epidermis by quantitative reverse-transcription PCR (RT-qPCR) and analyzing changes in skin immune cell subsets after UVB radiation using flow cytometry.For complete details on the use and execution of this protocol, please refer to Liu et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725001078GeneticsImmunologyModel Organisms |
| spellingShingle | Ye Liu George L. Sen Protocol for inducing epidermis-specific gene deletion and establishing a UVB-irradiated skin inflammation model STAR Protocols Genetics Immunology Model Organisms |
| title | Protocol for inducing epidermis-specific gene deletion and establishing a UVB-irradiated skin inflammation model |
| title_full | Protocol for inducing epidermis-specific gene deletion and establishing a UVB-irradiated skin inflammation model |
| title_fullStr | Protocol for inducing epidermis-specific gene deletion and establishing a UVB-irradiated skin inflammation model |
| title_full_unstemmed | Protocol for inducing epidermis-specific gene deletion and establishing a UVB-irradiated skin inflammation model |
| title_short | Protocol for inducing epidermis-specific gene deletion and establishing a UVB-irradiated skin inflammation model |
| title_sort | protocol for inducing epidermis specific gene deletion and establishing a uvb irradiated skin inflammation model |
| topic | Genetics Immunology Model Organisms |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725001078 |
| work_keys_str_mv | AT yeliu protocolforinducingepidermisspecificgenedeletionandestablishingauvbirradiatedskininflammationmodel AT georgelsen protocolforinducingepidermisspecificgenedeletionandestablishingauvbirradiatedskininflammationmodel |