Entomopathogenic fungi Metarhizium pingshaense increases susceptibility to insecticides in highly resistant malaria mosquitoes Anopheles coluzzii [version 2; peer review: 1 approved, 3 approved with reservations]
Background Metarhizium spp. based mosquito control products are among the most investigated and could potentially serve as promising complements to chemical insecticides. However, limited knowledge exists on the implementation of this biocontrol tool in conjunction with primary insecticide-based str...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wellcome
2025-02-01
|
| Series: | Wellcome Open Research |
| Subjects: | |
| Online Access: | https://wellcomeopenresearch.org/articles/9-290/v2 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850186433506050048 |
|---|---|
| author | Doubé Lucien Lamy Etienne Bilgo Abdoulaye Diabaté Issiaka Sare Edounou Jacques Gnambani Francesco Baldini Fatimata Aïcha Sodre Souro Abel Millogo Mafalda Viana Moussa Namountougou |
| author_facet | Doubé Lucien Lamy Etienne Bilgo Abdoulaye Diabaté Issiaka Sare Edounou Jacques Gnambani Francesco Baldini Fatimata Aïcha Sodre Souro Abel Millogo Mafalda Viana Moussa Namountougou |
| author_sort | Doubé Lucien Lamy |
| collection | DOAJ |
| description | Background Metarhizium spp. based mosquito control products are among the most investigated and could potentially serve as promising complements to chemical insecticides. However, limited knowledge exists on the implementation of this biocontrol tool in conjunction with primary insecticide-based strategies to achieve synergy. Methods In laboratory bioassays, we combined 107 conidia/ml native Metarhizium pingshaense strains with deltamethrin standard dose in three ways, before, after or simultaneously. These combinations were tested on laboratory insecticide resistant Anopheles coluzzii. Results We found that Metarhizium pingshaense and deltamethrin could be combined to achieve greater mortality against a highly insecticide resistance colony of Anopheles coluzzii. When mosquitoes were exposed to both simultaneously, no effect was observed, as expected for an insecticide resistant colony. However, when fungi were applied earlier than deltamethrin, mosquitoes became more sensitive to insecticide with a minimum Lethal Time to kill at least 50% of mosquito population (LT50) less than 8 days. In addition, when deltamethrin exposure was followed by Metarhizium infection, mosquito survival was similar to Metarhizium alone LT50 (LT50 ~11 days). Conclusion These findings suggest that early mosquito infection to Metarhizium pingshaense followed by chemical insecticide exposure synergically improve mosquito control in the laboratory. |
| format | Article |
| id | doaj-art-ca192b425a7f4b3e9672c258ca7320e4 |
| institution | OA Journals |
| issn | 2398-502X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Wellcome |
| record_format | Article |
| series | Wellcome Open Research |
| spelling | doaj-art-ca192b425a7f4b3e9672c258ca7320e42025-08-20T02:16:21ZengWellcomeWellcome Open Research2398-502X2025-02-01910.12688/wellcomeopenres.21238.226006Entomopathogenic fungi Metarhizium pingshaense increases susceptibility to insecticides in highly resistant malaria mosquitoes Anopheles coluzzii [version 2; peer review: 1 approved, 3 approved with reservations]Doubé Lucien Lamy0Etienne Bilgo1https://orcid.org/0000-0003-2350-9339Abdoulaye Diabaté2Issiaka Sare3Edounou Jacques Gnambani4Francesco Baldini5https://orcid.org/0000-0002-5904-4070Fatimata Aïcha Sodre6Souro Abel Millogo7Mafalda Viana8https://orcid.org/0000-0001-5975-6505Moussa Namountougou9Direction Régionale de l'Ouest, Institut de Recherche en Sciences de la Santé, Bobo-Dioulasso, Houet, 545, Burkina FasoDirection Régionale de l'Ouest, Institut de Recherche en Sciences de la Santé, Bobo-Dioulasso, Houet, 545, Burkina FasoDirection Régionale de l'Ouest, Institut de Recherche en Sciences de la Santé, Bobo-Dioulasso, Houet, 545, Burkina FasoDirection Régionale de l'Ouest, Institut de Recherche en Sciences de la Santé, Bobo-Dioulasso, Houet, 545, Burkina FasoDirection Régionale de l'Ouest, Institut de Recherche en Sciences de la Santé, Bobo-Dioulasso, Houet, 545, Burkina FasoSchool of Biodiversity One Health and Veterinary Medicine, University of Glasgow, Glasgow, Scotland, G12 8QQ, UKDirection Régionale de l'Ouest, Institut de Recherche en Sciences de la Santé, Bobo-Dioulasso, Houet, 545, Burkina FasoDirection Régionale de l'Ouest, Institut de Recherche en Sciences de la Santé, Bobo-Dioulasso, Houet, 545, Burkina FasoSchool of Biodiversity One Health and Veterinary Medicine, University of Glasgow, Glasgow, Scotland, G12 8QQ, UKLaboratoire de Recherches et d’Enseignements en Santé et Biotechnologies Animales (LARESBA), Universite NAZI BONI, Bobo-Dioulasso, Hauts-Bassins Region, 1091, Burkina FasoBackground Metarhizium spp. based mosquito control products are among the most investigated and could potentially serve as promising complements to chemical insecticides. However, limited knowledge exists on the implementation of this biocontrol tool in conjunction with primary insecticide-based strategies to achieve synergy. Methods In laboratory bioassays, we combined 107 conidia/ml native Metarhizium pingshaense strains with deltamethrin standard dose in three ways, before, after or simultaneously. These combinations were tested on laboratory insecticide resistant Anopheles coluzzii. Results We found that Metarhizium pingshaense and deltamethrin could be combined to achieve greater mortality against a highly insecticide resistance colony of Anopheles coluzzii. When mosquitoes were exposed to both simultaneously, no effect was observed, as expected for an insecticide resistant colony. However, when fungi were applied earlier than deltamethrin, mosquitoes became more sensitive to insecticide with a minimum Lethal Time to kill at least 50% of mosquito population (LT50) less than 8 days. In addition, when deltamethrin exposure was followed by Metarhizium infection, mosquito survival was similar to Metarhizium alone LT50 (LT50 ~11 days). Conclusion These findings suggest that early mosquito infection to Metarhizium pingshaense followed by chemical insecticide exposure synergically improve mosquito control in the laboratory.https://wellcomeopenresearch.org/articles/9-290/v2Metarhizium pingshaense Deltamethrin Anopheles coluzzii Insecticide resistance Integrated vector control Burkina Fasoeng |
| spellingShingle | Doubé Lucien Lamy Etienne Bilgo Abdoulaye Diabaté Issiaka Sare Edounou Jacques Gnambani Francesco Baldini Fatimata Aïcha Sodre Souro Abel Millogo Mafalda Viana Moussa Namountougou Entomopathogenic fungi Metarhizium pingshaense increases susceptibility to insecticides in highly resistant malaria mosquitoes Anopheles coluzzii [version 2; peer review: 1 approved, 3 approved with reservations] Wellcome Open Research Metarhizium pingshaense Deltamethrin Anopheles coluzzii Insecticide resistance Integrated vector control Burkina Faso eng |
| title | Entomopathogenic fungi Metarhizium pingshaense increases susceptibility to insecticides in highly resistant malaria mosquitoes Anopheles coluzzii [version 2; peer review: 1 approved, 3 approved with reservations] |
| title_full | Entomopathogenic fungi Metarhizium pingshaense increases susceptibility to insecticides in highly resistant malaria mosquitoes Anopheles coluzzii [version 2; peer review: 1 approved, 3 approved with reservations] |
| title_fullStr | Entomopathogenic fungi Metarhizium pingshaense increases susceptibility to insecticides in highly resistant malaria mosquitoes Anopheles coluzzii [version 2; peer review: 1 approved, 3 approved with reservations] |
| title_full_unstemmed | Entomopathogenic fungi Metarhizium pingshaense increases susceptibility to insecticides in highly resistant malaria mosquitoes Anopheles coluzzii [version 2; peer review: 1 approved, 3 approved with reservations] |
| title_short | Entomopathogenic fungi Metarhizium pingshaense increases susceptibility to insecticides in highly resistant malaria mosquitoes Anopheles coluzzii [version 2; peer review: 1 approved, 3 approved with reservations] |
| title_sort | entomopathogenic fungi metarhizium pingshaense increases susceptibility to insecticides in highly resistant malaria mosquitoes anopheles coluzzii version 2 peer review 1 approved 3 approved with reservations |
| topic | Metarhizium pingshaense Deltamethrin Anopheles coluzzii Insecticide resistance Integrated vector control Burkina Faso eng |
| url | https://wellcomeopenresearch.org/articles/9-290/v2 |
| work_keys_str_mv | AT doubelucienlamy entomopathogenicfungimetarhiziumpingshaenseincreasessusceptibilitytoinsecticidesinhighlyresistantmalariamosquitoesanophelescoluzziiversion2peerreview1approved3approvedwithreservations AT etiennebilgo entomopathogenicfungimetarhiziumpingshaenseincreasessusceptibilitytoinsecticidesinhighlyresistantmalariamosquitoesanophelescoluzziiversion2peerreview1approved3approvedwithreservations AT abdoulayediabate entomopathogenicfungimetarhiziumpingshaenseincreasessusceptibilitytoinsecticidesinhighlyresistantmalariamosquitoesanophelescoluzziiversion2peerreview1approved3approvedwithreservations AT issiakasare entomopathogenicfungimetarhiziumpingshaenseincreasessusceptibilitytoinsecticidesinhighlyresistantmalariamosquitoesanophelescoluzziiversion2peerreview1approved3approvedwithreservations AT edounoujacquesgnambani entomopathogenicfungimetarhiziumpingshaenseincreasessusceptibilitytoinsecticidesinhighlyresistantmalariamosquitoesanophelescoluzziiversion2peerreview1approved3approvedwithreservations AT francescobaldini entomopathogenicfungimetarhiziumpingshaenseincreasessusceptibilitytoinsecticidesinhighlyresistantmalariamosquitoesanophelescoluzziiversion2peerreview1approved3approvedwithreservations AT fatimataaichasodre entomopathogenicfungimetarhiziumpingshaenseincreasessusceptibilitytoinsecticidesinhighlyresistantmalariamosquitoesanophelescoluzziiversion2peerreview1approved3approvedwithreservations AT souroabelmillogo entomopathogenicfungimetarhiziumpingshaenseincreasessusceptibilitytoinsecticidesinhighlyresistantmalariamosquitoesanophelescoluzziiversion2peerreview1approved3approvedwithreservations AT mafaldaviana entomopathogenicfungimetarhiziumpingshaenseincreasessusceptibilitytoinsecticidesinhighlyresistantmalariamosquitoesanophelescoluzziiversion2peerreview1approved3approvedwithreservations AT moussanamountougou entomopathogenicfungimetarhiziumpingshaenseincreasessusceptibilitytoinsecticidesinhighlyresistantmalariamosquitoesanophelescoluzziiversion2peerreview1approved3approvedwithreservations |