3D printing‐based frugal manufacturing of glass pipettes for minimally invasive delivery of therapeutics to the brain
Abstract Objective Intracerebral delivery of agents in liquid form is usually achieved through commercially available and durable metal needles. However, their size and texture may contribute to mechanical brain damage. Glass pipettes with a thin tip may significantly reduce injection‐associated bra...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-09-01
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| Series: | Neuroprotection |
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| Online Access: | https://doi.org/10.1002/nep3.20 |
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| author | Guanda Qiao David Gulisashvili Anna Jablonska Guiling Zhao Miroslaw Janowski Piotr Walczak Yajie Liang |
| author_facet | Guanda Qiao David Gulisashvili Anna Jablonska Guiling Zhao Miroslaw Janowski Piotr Walczak Yajie Liang |
| author_sort | Guanda Qiao |
| collection | DOAJ |
| description | Abstract Objective Intracerebral delivery of agents in liquid form is usually achieved through commercially available and durable metal needles. However, their size and texture may contribute to mechanical brain damage. Glass pipettes with a thin tip may significantly reduce injection‐associated brain damage but require access to prohibitively expensive programmable pipette pullers. This study is to remove the economic barrier to the application of minimally invasive delivery of therapeutics to the brain, such as chemical compounds, viral vectors, and cells. Methods We took advantage of the rapid development of free educational online resources and emerging low‐cost 3D printers by designing an affordable pipette puller (APP) to remove the cost obstacle. Results We showed that our APP could produce glass pipettes with a sharp tip opening down to 20 μm or less, which is sufficiently thin for the delivery of therapeutics into the brain. A pipeline from pipette pulling to brain injection using low‐cost and open‐source equipment was established to facilitate the application of the APP. Conclusion In the spirit of frugal science, our device may democratize glass pipette‐puling and substantially promote the application of minimally invasive and precisely controlled delivery of therapeutics to the brain for finding more effective therapies of brain diseases. |
| format | Article |
| id | doaj-art-ca17019b8ce84a09b704501514be89f7 |
| institution | DOAJ |
| issn | 2770-7296 2770-730X |
| language | English |
| publishDate | 2023-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neuroprotection |
| spelling | doaj-art-ca17019b8ce84a09b704501514be89f72025-08-20T03:13:12ZengWileyNeuroprotection2770-72962770-730X2023-09-0111354210.1002/nep3.203D printing‐based frugal manufacturing of glass pipettes for minimally invasive delivery of therapeutics to the brainGuanda Qiao0David Gulisashvili1Anna Jablonska2Guiling Zhao3Miroslaw Janowski4Piotr Walczak5Yajie Liang6Department of Diagnostic Radiology and Nuclear Medicine University of Maryland School of Medicine Baltimore Maryland USADepartment of Diagnostic Radiology and Nuclear Medicine University of Maryland School of Medicine Baltimore Maryland USADepartment of Diagnostic Radiology and Nuclear Medicine University of Maryland School of Medicine Baltimore Maryland USALaboratory of Molecular Cardiology, Department of Physiology, Center for Biomedical Engineering and Technology University of Maryland School of Medicine Baltimore Maryland USADepartment of Diagnostic Radiology and Nuclear Medicine University of Maryland School of Medicine Baltimore Maryland USADepartment of Diagnostic Radiology and Nuclear Medicine University of Maryland School of Medicine Baltimore Maryland USADepartment of Diagnostic Radiology and Nuclear Medicine University of Maryland School of Medicine Baltimore Maryland USAAbstract Objective Intracerebral delivery of agents in liquid form is usually achieved through commercially available and durable metal needles. However, their size and texture may contribute to mechanical brain damage. Glass pipettes with a thin tip may significantly reduce injection‐associated brain damage but require access to prohibitively expensive programmable pipette pullers. This study is to remove the economic barrier to the application of minimally invasive delivery of therapeutics to the brain, such as chemical compounds, viral vectors, and cells. Methods We took advantage of the rapid development of free educational online resources and emerging low‐cost 3D printers by designing an affordable pipette puller (APP) to remove the cost obstacle. Results We showed that our APP could produce glass pipettes with a sharp tip opening down to 20 μm or less, which is sufficiently thin for the delivery of therapeutics into the brain. A pipeline from pipette pulling to brain injection using low‐cost and open‐source equipment was established to facilitate the application of the APP. Conclusion In the spirit of frugal science, our device may democratize glass pipette‐puling and substantially promote the application of minimally invasive and precisely controlled delivery of therapeutics to the brain for finding more effective therapies of brain diseases.https://doi.org/10.1002/nep3.203D‐printablebrain injectionfrugal scienceglass pipette pulling |
| spellingShingle | Guanda Qiao David Gulisashvili Anna Jablonska Guiling Zhao Miroslaw Janowski Piotr Walczak Yajie Liang 3D printing‐based frugal manufacturing of glass pipettes for minimally invasive delivery of therapeutics to the brain Neuroprotection 3D‐printable brain injection frugal science glass pipette pulling |
| title | 3D printing‐based frugal manufacturing of glass pipettes for minimally invasive delivery of therapeutics to the brain |
| title_full | 3D printing‐based frugal manufacturing of glass pipettes for minimally invasive delivery of therapeutics to the brain |
| title_fullStr | 3D printing‐based frugal manufacturing of glass pipettes for minimally invasive delivery of therapeutics to the brain |
| title_full_unstemmed | 3D printing‐based frugal manufacturing of glass pipettes for minimally invasive delivery of therapeutics to the brain |
| title_short | 3D printing‐based frugal manufacturing of glass pipettes for minimally invasive delivery of therapeutics to the brain |
| title_sort | 3d printing based frugal manufacturing of glass pipettes for minimally invasive delivery of therapeutics to the brain |
| topic | 3D‐printable brain injection frugal science glass pipette pulling |
| url | https://doi.org/10.1002/nep3.20 |
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