Dietary supplementation of Lycium barbarum polysaccharides alleviates soybean meal-induced enteritis in spotted sea bass Lateolabrax maculatus
The aim of this experiment was to investigate the effect of Lycium barbarum polysaccharides (LBP) on alleviating soybean meal-induced enteritis (SBMIE) in spotted sea bass Lateolabrax maculatus. The diet with 44% fishmeal (FM) content was used as a blank control, and soybean meal (SM) was used to re...
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KeAi Communications Co., Ltd.
2025-03-01
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| Series: | Animal Nutrition |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405654524001537 |
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| author | Longhui Liu Yanbo Zhao Zhangfan Huang Zhongying Long Huihui Qin Hao Lin Sishun Zhou Lumin Kong Jianrong Ma Yi Lin Zhongbao Li |
| author_facet | Longhui Liu Yanbo Zhao Zhangfan Huang Zhongying Long Huihui Qin Hao Lin Sishun Zhou Lumin Kong Jianrong Ma Yi Lin Zhongbao Li |
| author_sort | Longhui Liu |
| collection | DOAJ |
| description | The aim of this experiment was to investigate the effect of Lycium barbarum polysaccharides (LBP) on alleviating soybean meal-induced enteritis (SBMIE) in spotted sea bass Lateolabrax maculatus. The diet with 44% fishmeal (FM) content was used as a blank control, and soybean meal (SM) was used to replace 50% FM as an experimental control to induce enteritis. Then, on the basis of experimental control, 0.10%, 0.15%, and 0.20% LBP were added as experimental diets. A total of 225 spotted sea bass (44.52 ± 0.24 g) were randomly divided into 5 groups and fed the corresponding diets for 52 d. The results showed that 0.15% LBP decreased serum D-lactic acid (D-LA) content and diamine oxidase (DAO) activity (P < 0.05). In addition, in all LBP supplementation groups, the intestinal tissue morphology was significantly improved (P < 0.05); the intestinal microbial structure gradually recovered to a level close to that without adding SM; and the microbial species richness and diversity were significantly increased (P < 0.05). Through transcriptomic and metabolomic analysis, it was found that the expression of proinflammatory factors such as interleukin-1β (IL-1β), interleukin-12 (IL-12), nuclear factor kappa B subunit 2 (NF-κB2), and Toll-like receptor 2 (TLR2) were significantly down-regulated in the mitogen-activated protein kinase (MAPK) and Toll-like receptor signaling pathways (P < 0.05), and the important tight junction protein gene Occludin was up-regulated (P < 0.05). In addition, LBP down-regulated saponin metabolites and up-regulated amino acid metabolites (P < 0.05). In conclusion, LBP demonstrated a significant alleviating effect on SBMIE of spotted sea bass L. maculatus. |
| format | Article |
| id | doaj-art-ca0e195e9c5e43d68a70d379f584dd47 |
| institution | Kabale University |
| issn | 2405-6545 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Animal Nutrition |
| spelling | doaj-art-ca0e195e9c5e43d68a70d379f584dd472025-01-31T05:11:57ZengKeAi Communications Co., Ltd.Animal Nutrition2405-65452025-03-0120122Dietary supplementation of Lycium barbarum polysaccharides alleviates soybean meal-induced enteritis in spotted sea bass Lateolabrax maculatusLonghui Liu0Yanbo Zhao1Zhangfan Huang2Zhongying Long3Huihui Qin4Hao Lin5Sishun Zhou6Lumin Kong7Jianrong Ma8Yi Lin9Zhongbao Li10Fisheries College, Jimei University, Xiamen, China; Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, ChinaFisheries College, Jimei University, Xiamen, China; Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, ChinaFisheries College, Jimei University, Xiamen, China; Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, ChinaFisheries College, Jimei University, Xiamen, China; Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, ChinaFisheries College, Jimei University, Xiamen, China; Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, ChinaFisheries College, Jimei University, Xiamen, China; Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, ChinaFisheries College, Jimei University, Xiamen, China; Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, ChinaFisheries College, Jimei University, Xiamen, China; Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, ChinaFisheries College, Jimei University, Xiamen, China; Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, ChinaFisheries College, Jimei University, Xiamen, China; Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, ChinaFisheries College, Jimei University, Xiamen, China; Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, China; Corresponding author.The aim of this experiment was to investigate the effect of Lycium barbarum polysaccharides (LBP) on alleviating soybean meal-induced enteritis (SBMIE) in spotted sea bass Lateolabrax maculatus. The diet with 44% fishmeal (FM) content was used as a blank control, and soybean meal (SM) was used to replace 50% FM as an experimental control to induce enteritis. Then, on the basis of experimental control, 0.10%, 0.15%, and 0.20% LBP were added as experimental diets. A total of 225 spotted sea bass (44.52 ± 0.24 g) were randomly divided into 5 groups and fed the corresponding diets for 52 d. The results showed that 0.15% LBP decreased serum D-lactic acid (D-LA) content and diamine oxidase (DAO) activity (P < 0.05). In addition, in all LBP supplementation groups, the intestinal tissue morphology was significantly improved (P < 0.05); the intestinal microbial structure gradually recovered to a level close to that without adding SM; and the microbial species richness and diversity were significantly increased (P < 0.05). Through transcriptomic and metabolomic analysis, it was found that the expression of proinflammatory factors such as interleukin-1β (IL-1β), interleukin-12 (IL-12), nuclear factor kappa B subunit 2 (NF-κB2), and Toll-like receptor 2 (TLR2) were significantly down-regulated in the mitogen-activated protein kinase (MAPK) and Toll-like receptor signaling pathways (P < 0.05), and the important tight junction protein gene Occludin was up-regulated (P < 0.05). In addition, LBP down-regulated saponin metabolites and up-regulated amino acid metabolites (P < 0.05). In conclusion, LBP demonstrated a significant alleviating effect on SBMIE of spotted sea bass L. maculatus.http://www.sciencedirect.com/science/article/pii/S2405654524001537Lycium barbarum polysaccharidesSpotted sea bassIntestinal inflammationTranscriptomics and metabolomics |
| spellingShingle | Longhui Liu Yanbo Zhao Zhangfan Huang Zhongying Long Huihui Qin Hao Lin Sishun Zhou Lumin Kong Jianrong Ma Yi Lin Zhongbao Li Dietary supplementation of Lycium barbarum polysaccharides alleviates soybean meal-induced enteritis in spotted sea bass Lateolabrax maculatus Animal Nutrition Lycium barbarum polysaccharides Spotted sea bass Intestinal inflammation Transcriptomics and metabolomics |
| title | Dietary supplementation of Lycium barbarum polysaccharides alleviates soybean meal-induced enteritis in spotted sea bass Lateolabrax maculatus |
| title_full | Dietary supplementation of Lycium barbarum polysaccharides alleviates soybean meal-induced enteritis in spotted sea bass Lateolabrax maculatus |
| title_fullStr | Dietary supplementation of Lycium barbarum polysaccharides alleviates soybean meal-induced enteritis in spotted sea bass Lateolabrax maculatus |
| title_full_unstemmed | Dietary supplementation of Lycium barbarum polysaccharides alleviates soybean meal-induced enteritis in spotted sea bass Lateolabrax maculatus |
| title_short | Dietary supplementation of Lycium barbarum polysaccharides alleviates soybean meal-induced enteritis in spotted sea bass Lateolabrax maculatus |
| title_sort | dietary supplementation of lycium barbarum polysaccharides alleviates soybean meal induced enteritis in spotted sea bass lateolabrax maculatus |
| topic | Lycium barbarum polysaccharides Spotted sea bass Intestinal inflammation Transcriptomics and metabolomics |
| url | http://www.sciencedirect.com/science/article/pii/S2405654524001537 |
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