Recombinant Bombyx mori Nucleopolyhedrovirus Harboring Green Fluorescent Protein
The gene encoding the green fluorescent protein (gfp) under the control of the highly expressed Autographa californica nucleopolyhedrovirus (AcMNPV)-polyhedrin promoter has been introduced into the polyhedron (polh) locus of Bombyx mori nucleopolyhedrovirus (BmNPV) by homologous recombination. The i...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
1998-12-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/98256st02 |
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| author | Deepak Sehgal Karumathil P. Gopinathan |
| author_facet | Deepak Sehgal Karumathil P. Gopinathan |
| author_sort | Deepak Sehgal |
| collection | DOAJ |
| description | The gene encoding the green fluorescent protein (gfp) under the control of the highly expressed Autographa californica nucleopolyhedrovirus (AcMNPV)-polyhedrin promoter has been introduced into the polyhedron (polh) locus of Bombyx mori nucleopolyhedrovirus (BmNPV) by homologous recombination. The insect host larvae and the cultured cells infected with this recombinant virus (vBmGFP) showed high levels of expression of gfp. The larval tissues permissive to virus multiplication could be readily visualized using the tagged recombinant virus, thus providing a direct approach to study the progress of virus infection or its control in the animal host. The highly expressed recombinant protein, GFP, could be easily solubilized from fat bodies. Thus, the caterpillar-based expression could serve as an economic alternative method for the large-scale production of recombinant proteins, even when they are nonsecretory in nature. Further, if the recombinant vBmGFP is used as a parent in generating other recombinants, conversion of the fluorescent plaques to colorless plaques serves as an easy means for screening recombinants. Such a method is especially helpful for BmNPV-recombinant selections in the absence of the other simplified techniques as are available for the prototype baculovirus AcMNPV system. |
| format | Article |
| id | doaj-art-ca0dde5f50244e1ab11bdbbd5d84e223 |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 1998-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-ca0dde5f50244e1ab11bdbbd5d84e2232025-08-20T02:25:37ZengTaylor & Francis GroupBioTechniques0736-62051940-98181998-12-01256997100610.2144/98256st02Recombinant Bombyx mori Nucleopolyhedrovirus Harboring Green Fluorescent ProteinDeepak Sehgal0Karumathil P. Gopinathan11Indian Institute of Science, Bangalore, India1Indian Institute of Science, Bangalore, IndiaThe gene encoding the green fluorescent protein (gfp) under the control of the highly expressed Autographa californica nucleopolyhedrovirus (AcMNPV)-polyhedrin promoter has been introduced into the polyhedron (polh) locus of Bombyx mori nucleopolyhedrovirus (BmNPV) by homologous recombination. The insect host larvae and the cultured cells infected with this recombinant virus (vBmGFP) showed high levels of expression of gfp. The larval tissues permissive to virus multiplication could be readily visualized using the tagged recombinant virus, thus providing a direct approach to study the progress of virus infection or its control in the animal host. The highly expressed recombinant protein, GFP, could be easily solubilized from fat bodies. Thus, the caterpillar-based expression could serve as an economic alternative method for the large-scale production of recombinant proteins, even when they are nonsecretory in nature. Further, if the recombinant vBmGFP is used as a parent in generating other recombinants, conversion of the fluorescent plaques to colorless plaques serves as an easy means for screening recombinants. Such a method is especially helpful for BmNPV-recombinant selections in the absence of the other simplified techniques as are available for the prototype baculovirus AcMNPV system.https://www.future-science.com/doi/10.2144/98256st02 |
| spellingShingle | Deepak Sehgal Karumathil P. Gopinathan Recombinant Bombyx mori Nucleopolyhedrovirus Harboring Green Fluorescent Protein BioTechniques |
| title | Recombinant Bombyx mori Nucleopolyhedrovirus Harboring Green Fluorescent Protein |
| title_full | Recombinant Bombyx mori Nucleopolyhedrovirus Harboring Green Fluorescent Protein |
| title_fullStr | Recombinant Bombyx mori Nucleopolyhedrovirus Harboring Green Fluorescent Protein |
| title_full_unstemmed | Recombinant Bombyx mori Nucleopolyhedrovirus Harboring Green Fluorescent Protein |
| title_short | Recombinant Bombyx mori Nucleopolyhedrovirus Harboring Green Fluorescent Protein |
| title_sort | recombinant bombyx mori nucleopolyhedrovirus harboring green fluorescent protein |
| url | https://www.future-science.com/doi/10.2144/98256st02 |
| work_keys_str_mv | AT deepaksehgal recombinantbombyxmorinucleopolyhedrovirusharboringgreenfluorescentprotein AT karumathilpgopinathan recombinantbombyxmorinucleopolyhedrovirusharboringgreenfluorescentprotein |