Microsatellite Instability and BAT-26 Marker Expression in a Mexican Prostate Cancer Population with Different Gleason Scores

Background/Objectives: Prostate cancer (PCa) is one of the most common cancers in men worldwide. While standard treatments often provide good initial results, many patients eventually develop resistance and experience a more aggressive relapse. Microsatellite instability (MSI) involves variations in...

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Main Authors: Ana K. Flores-Islas, Manuel A. Rico-Méndez, Marisol Godínez-Rubí, Martha Arisbeth Villanueva-Pérez, Erick Sierra-Díaz, Ana Laura Pereira-Suárez, Saul A. Beltrán-Ontiveros, Perla Y. Gutiérrez-Arzapalo, José M. Moreno-Ortiz, Adrián Ramírez-de-Arellano
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Diseases
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Online Access:https://www.mdpi.com/2079-9721/13/7/202
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author Ana K. Flores-Islas
Manuel A. Rico-Méndez
Marisol Godínez-Rubí
Martha Arisbeth Villanueva-Pérez
Erick Sierra-Díaz
Ana Laura Pereira-Suárez
Saul A. Beltrán-Ontiveros
Perla Y. Gutiérrez-Arzapalo
José M. Moreno-Ortiz
Adrián Ramírez-de-Arellano
author_facet Ana K. Flores-Islas
Manuel A. Rico-Méndez
Marisol Godínez-Rubí
Martha Arisbeth Villanueva-Pérez
Erick Sierra-Díaz
Ana Laura Pereira-Suárez
Saul A. Beltrán-Ontiveros
Perla Y. Gutiérrez-Arzapalo
José M. Moreno-Ortiz
Adrián Ramírez-de-Arellano
author_sort Ana K. Flores-Islas
collection DOAJ
description Background/Objectives: Prostate cancer (PCa) is one of the most common cancers in men worldwide. While standard treatments often provide good initial results, many patients eventually develop resistance and experience a more aggressive relapse. Microsatellite instability (MSI) involves variations in the lengths of microsatellite base repeats in cells. Assessing the frequency of MSI is essential, as it may identify candidates for immune checkpoint inhibitors, which have shown promising outcomes. This study focuses on evaluating the MSI frequency in Mexican PCa patients and exploring its potential relationship with tumor aggressiveness. Methods: In this study, 116 formalin-fixed paraffin-embedded tumoral tissue biopsies from Mexican patients with PCa were collected from Hospital Civil de Culiacán and Pathology and Nephropathology, Diagnosis and Research Center, in the period from 2021 to 2024. The Gleason score was assessed, and the MSI was performed by multiplex PCR with a panel of five markers (NR-27, NR-21, NR-24, BAT-25, and BAT-26). High microsatellite instability (MSI-H) was defined as two or more unstable markers, low microsatellite instability (MSI-L) as an unstable marker, and microsatellite stability (MSS) as no unstable marker. Results: We found 19.83% (23/116) MSI PCa patients, of which 21.74% (5/23) were MSI-H, and 78.26% (18/23) were MSI-L. We found a major distribution of MSI-positive cases (50% (11/22)) in Gleason score 9 patients, corresponding to prognostic group 5. In addition, we found most of the instability in the BAT-26 marker in MSI PCa patients (60.87% (14/23)). Conclusions: This study is the first to evaluate the frequency of MSI in PCa within the Mexican population. Among the Mexican patients with MSI-positive PCa, there was a predominant Gleason score 9 and a majority instability of the BAT-26 marker.
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spelling doaj-art-c9fc375f700e46e08c8174a5ee0d2abe2025-08-20T03:35:37ZengMDPI AGDiseases2079-97212025-06-0113720210.3390/diseases13070202Microsatellite Instability and BAT-26 Marker Expression in a Mexican Prostate Cancer Population with Different Gleason ScoresAna K. Flores-Islas0Manuel A. Rico-Méndez1Marisol Godínez-Rubí2Martha Arisbeth Villanueva-Pérez3Erick Sierra-Díaz4Ana Laura Pereira-Suárez5Saul A. Beltrán-Ontiveros6Perla Y. Gutiérrez-Arzapalo7José M. Moreno-Ortiz8Adrián Ramírez-de-Arellano9Laboratorio de Investigación en Cáncer e Infecciones, Departamento de Microbiología y Patología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara 44340, Jalisco, MexicoInstituto de Genética Humana “Dr. Enrique Corona Rivera”, Departamento de Biología Molecular y Genómica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara 44340, Jalisco, MexicoLaboratorio de Patología Diagnóstica e Inmunohistoquímica, Centro de Investigación y Diagnóstico en Patología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara 44340, Jalisco, MexicoPatología y Nefropatología, Centro de Diagnóstico e Investigación, Guadalajara 44600, Jalisco, MexicoUnidad Médica de Alta Especialidad, Hospital de Especialidades, Centro Médico Nacional de Occidente, Universidad de Guadalajara, Guadalajara 44340, Jalisco, MexicoLaboratorio de Investigación en Cáncer e Infecciones, Departamento de Microbiología y Patología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara 44340, Jalisco, MexicoCentro de Investigación y Docencia en Ciencias de la Salud, Universidad Autónoma de Sinaloa, Culiacán Rosales 80030, Sinaloa, MexicoCentro de Investigación y Docencia en Ciencias de la Salud, Universidad Autónoma de Sinaloa, Culiacán Rosales 80030, Sinaloa, MexicoInstituto de Genética Humana “Dr. Enrique Corona Rivera”, Departamento de Biología Molecular y Genómica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara 44340, Jalisco, MexicoLaboratorio de Investigación en Cáncer e Infecciones, Departamento de Microbiología y Patología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara 44340, Jalisco, MexicoBackground/Objectives: Prostate cancer (PCa) is one of the most common cancers in men worldwide. While standard treatments often provide good initial results, many patients eventually develop resistance and experience a more aggressive relapse. Microsatellite instability (MSI) involves variations in the lengths of microsatellite base repeats in cells. Assessing the frequency of MSI is essential, as it may identify candidates for immune checkpoint inhibitors, which have shown promising outcomes. This study focuses on evaluating the MSI frequency in Mexican PCa patients and exploring its potential relationship with tumor aggressiveness. Methods: In this study, 116 formalin-fixed paraffin-embedded tumoral tissue biopsies from Mexican patients with PCa were collected from Hospital Civil de Culiacán and Pathology and Nephropathology, Diagnosis and Research Center, in the period from 2021 to 2024. The Gleason score was assessed, and the MSI was performed by multiplex PCR with a panel of five markers (NR-27, NR-21, NR-24, BAT-25, and BAT-26). High microsatellite instability (MSI-H) was defined as two or more unstable markers, low microsatellite instability (MSI-L) as an unstable marker, and microsatellite stability (MSS) as no unstable marker. Results: We found 19.83% (23/116) MSI PCa patients, of which 21.74% (5/23) were MSI-H, and 78.26% (18/23) were MSI-L. We found a major distribution of MSI-positive cases (50% (11/22)) in Gleason score 9 patients, corresponding to prognostic group 5. In addition, we found most of the instability in the BAT-26 marker in MSI PCa patients (60.87% (14/23)). Conclusions: This study is the first to evaluate the frequency of MSI in PCa within the Mexican population. Among the Mexican patients with MSI-positive PCa, there was a predominant Gleason score 9 and a majority instability of the BAT-26 marker.https://www.mdpi.com/2079-9721/13/7/202prostate cancermicrosatellite instabilityGleason scorepersonalized medicineimmunotherapyMexico
spellingShingle Ana K. Flores-Islas
Manuel A. Rico-Méndez
Marisol Godínez-Rubí
Martha Arisbeth Villanueva-Pérez
Erick Sierra-Díaz
Ana Laura Pereira-Suárez
Saul A. Beltrán-Ontiveros
Perla Y. Gutiérrez-Arzapalo
José M. Moreno-Ortiz
Adrián Ramírez-de-Arellano
Microsatellite Instability and BAT-26 Marker Expression in a Mexican Prostate Cancer Population with Different Gleason Scores
Diseases
prostate cancer
microsatellite instability
Gleason score
personalized medicine
immunotherapy
Mexico
title Microsatellite Instability and BAT-26 Marker Expression in a Mexican Prostate Cancer Population with Different Gleason Scores
title_full Microsatellite Instability and BAT-26 Marker Expression in a Mexican Prostate Cancer Population with Different Gleason Scores
title_fullStr Microsatellite Instability and BAT-26 Marker Expression in a Mexican Prostate Cancer Population with Different Gleason Scores
title_full_unstemmed Microsatellite Instability and BAT-26 Marker Expression in a Mexican Prostate Cancer Population with Different Gleason Scores
title_short Microsatellite Instability and BAT-26 Marker Expression in a Mexican Prostate Cancer Population with Different Gleason Scores
title_sort microsatellite instability and bat 26 marker expression in a mexican prostate cancer population with different gleason scores
topic prostate cancer
microsatellite instability
Gleason score
personalized medicine
immunotherapy
Mexico
url https://www.mdpi.com/2079-9721/13/7/202
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