Seroprevalence of SARS-COV-2 antibodies among school-age children in the Swabi district of Pakistan

OBJECTIVES: To determine the seroprevalence of SARS-CoV-2 antibodies (IgM, IgG) in school-age children and identify the predominant symptoms in seropositive children in the Swabi district of Khyber Pakhtunkhwa, Pakistan. METHODS: This descriptive cross-sectional study was conducted from November 2...

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Main Authors: Muhammad Arif, Muhammad Nisar Khan, Muhammad Ibrahim, Syed Abbas Anwar, Sumera, Yasar Mehmood Yousafzai
Format: Article
Language:English
Published: Khyber Medical University 2024-03-01
Series:Khyber Medical University Journal
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Online Access:https://www.kmuj.kmu.edu.pk/article/view/23504
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author Muhammad Arif
Muhammad Nisar Khan
Muhammad Ibrahim
Syed Abbas Anwar
Sumera
Yasar Mehmood Yousafzai
author_facet Muhammad Arif
Muhammad Nisar Khan
Muhammad Ibrahim
Syed Abbas Anwar
Sumera
Yasar Mehmood Yousafzai
author_sort Muhammad Arif
collection DOAJ
description OBJECTIVES: To determine the seroprevalence of SARS-CoV-2 antibodies (IgM, IgG) in school-age children and identify the predominant symptoms in seropositive children in the Swabi district of Khyber Pakhtunkhwa, Pakistan. METHODS: This descriptive cross-sectional study was conducted from November 2020 to May 2021. Blood samples from 246 randomly selected children (aged 5-16 years) in Swabi district of Pakistan were analyzed using ELISA. The study focused on the general pediatric population, excluding those over 16 years, with chronic illnesses, or whose parents declined participation. Data was collected through a structured questionnaire. RESULTS: Among 246 participants, 5 (2.03%) individuals tested positive for IgM and 58 (23.6%) for IgG antibodies, totaling 63 (25.6%) participants with both IgM & IgG antibodies. Mean age of seropositive and seronegative children was 11.2±3.0 and 10.3±2.98 years respectively (p=0.030). Out of 63 seropositive children, 51 (80.96%) were symptomatic, and 12 (19.04%) were asymptomatic. Common symptoms among seropositive individuals included fever (98.0%, n=50/51), cough (88.02%, n=45/51), sore throat (78.4%, n=40/51), coryza (66.7%, n=34/51), myalgia (60.8%, n=31/51), and loss of smell and taste (49.0%, n=25/51). The mean antibody titer was 20.9±10.41 IU/ml and 13.35±5.6 IU/ml for symptomatic and asymptomatic individuals respectively (p=0.013). CONCLUSION: In our study, approximately one fourth of the participants tested positive for SARS-CoV-2 antibodies, predominantly IgG. Females exhibited higher seropositivity than males, with a significant association between seropositivity and exposure to confirmed COVID-19 cases. Symptomatic cases comprised 80.95% of seropositive individuals, with predominant symptoms of fever, cough, and sore throat. Symptomatic individuals had a higher mean antibody titer.
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publishDate 2024-03-01
publisher Khyber Medical University
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series Khyber Medical University Journal
spelling doaj-art-c9f905749b444c869f0478be8ebbe09e2025-08-20T01:47:21ZengKhyber Medical UniversityKhyber Medical University Journal2305-26432305-26512024-03-01161101510.35845/kmuj.2024.235042276Seroprevalence of SARS-COV-2 antibodies among school-age children in the Swabi district of PakistanMuhammad Arif0Muhammad Nisar Khan1Muhammad Ibrahim2Syed Abbas Anwar3Sumera4Yasar Mehmood Yousafzai5Institute of Pathology and Diagnostic Medicine, Khyber Medical University, Peshawar, PakistanInstitute of Pathology and Diagnostic Medicine, Khyber Medical University, Peshawar, Pakistan and Regional Blood Centre, Peshawar, PakistanInstitute of Pathology and Diagnostic Medicine, Khyber Medical University, Peshawar, PakistanInstitute of Pathology and Diagnostic Medicine, Khyber Medical University, Peshawar, Pakistan and Ayub Teaching Hospital, AbbottabadInstitute of Pathology and diagnostic Medicine, Khyber Medical University, PeshawarInstitute of Pathology and Diagnostic Medicine, Khyber Medical University, Peshawar, PakistanOBJECTIVES: To determine the seroprevalence of SARS-CoV-2 antibodies (IgM, IgG) in school-age children and identify the predominant symptoms in seropositive children in the Swabi district of Khyber Pakhtunkhwa, Pakistan. METHODS: This descriptive cross-sectional study was conducted from November 2020 to May 2021. Blood samples from 246 randomly selected children (aged 5-16 years) in Swabi district of Pakistan were analyzed using ELISA. The study focused on the general pediatric population, excluding those over 16 years, with chronic illnesses, or whose parents declined participation. Data was collected through a structured questionnaire. RESULTS: Among 246 participants, 5 (2.03%) individuals tested positive for IgM and 58 (23.6%) for IgG antibodies, totaling 63 (25.6%) participants with both IgM & IgG antibodies. Mean age of seropositive and seronegative children was 11.2±3.0 and 10.3±2.98 years respectively (p=0.030). Out of 63 seropositive children, 51 (80.96%) were symptomatic, and 12 (19.04%) were asymptomatic. Common symptoms among seropositive individuals included fever (98.0%, n=50/51), cough (88.02%, n=45/51), sore throat (78.4%, n=40/51), coryza (66.7%, n=34/51), myalgia (60.8%, n=31/51), and loss of smell and taste (49.0%, n=25/51). The mean antibody titer was 20.9±10.41 IU/ml and 13.35±5.6 IU/ml for symptomatic and asymptomatic individuals respectively (p=0.013). CONCLUSION: In our study, approximately one fourth of the participants tested positive for SARS-CoV-2 antibodies, predominantly IgG. Females exhibited higher seropositivity than males, with a significant association between seropositivity and exposure to confirmed COVID-19 cases. Symptomatic cases comprised 80.95% of seropositive individuals, with predominant symptoms of fever, cough, and sore throat. Symptomatic individuals had a higher mean antibody titer.https://www.kmuj.kmu.edu.pk/article/view/23504covid-19seroprevalenceimmunityserosurveyimmune surveillancesars-cov-2
spellingShingle Muhammad Arif
Muhammad Nisar Khan
Muhammad Ibrahim
Syed Abbas Anwar
Sumera
Yasar Mehmood Yousafzai
Seroprevalence of SARS-COV-2 antibodies among school-age children in the Swabi district of Pakistan
Khyber Medical University Journal
covid-19
seroprevalence
immunity
serosurvey
immune surveillance
sars-cov-2
title Seroprevalence of SARS-COV-2 antibodies among school-age children in the Swabi district of Pakistan
title_full Seroprevalence of SARS-COV-2 antibodies among school-age children in the Swabi district of Pakistan
title_fullStr Seroprevalence of SARS-COV-2 antibodies among school-age children in the Swabi district of Pakistan
title_full_unstemmed Seroprevalence of SARS-COV-2 antibodies among school-age children in the Swabi district of Pakistan
title_short Seroprevalence of SARS-COV-2 antibodies among school-age children in the Swabi district of Pakistan
title_sort seroprevalence of sars cov 2 antibodies among school age children in the swabi district of pakistan
topic covid-19
seroprevalence
immunity
serosurvey
immune surveillance
sars-cov-2
url https://www.kmuj.kmu.edu.pk/article/view/23504
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