Antiproliferative Effect of Grammatophyllum speciosum Ethanolic Extract and Its Bioactive Compound on Human Breast Cancer Cells

Background/Aim. Grammatophyllum speciosum Blume exhibits various promising pharmacological activities. However, its effect on breast cancer has not been determined. Materials and Methods. The antiproliferation effects of the G. speciosum pseudobulb ethanolic extract (GSE) and isovitexin (bioactive c...

Full description

Saved in:
Bibliographic Details
Main Authors: Verisa Chowjarean, Kamala Sadabpod
Format: Article
Language:English
Published: Wiley 2021-01-01
Series:The Scientific World Journal
Online Access:http://dx.doi.org/10.1155/2021/3752169
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832546220215631872
author Verisa Chowjarean
Kamala Sadabpod
author_facet Verisa Chowjarean
Kamala Sadabpod
author_sort Verisa Chowjarean
collection DOAJ
description Background/Aim. Grammatophyllum speciosum Blume exhibits various promising pharmacological activities. However, its effect on breast cancer has not been determined. Materials and Methods. The antiproliferation effects of the G. speciosum pseudobulb ethanolic extract (GSE) and isovitexin (bioactive constituent) were investigated on the MCF-7 human breast cancer cell line using MTT and colony formation assay. The expression levels of proliferation-regulatory proteins were determined by western blotting. Results. Noncytotoxic concentrations of GSE significantly suppressed the proliferation of MCF-7 cells. Tumor colony formation decreased in both number and size. The level of phosphorylated AKT and β-catenin was suppressed by GSE treatment. Antiproliferation was observed in isovitexin-treated MCF-7 cells in the form of inhibited colony formation and reduced expression of phosphorylated AKT and β-catenin protein. Conclusions. This study demonstrates the novel effect of G. speciosum as an antiproliferative via suppression of the AKT/β-catenin-dependent pathway. This may prompt further investigation of this plant in breast cancer therapy.
format Article
id doaj-art-c9f7842b26fa4fc4a316bf950b50ceac
institution Kabale University
issn 2356-6140
1537-744X
language English
publishDate 2021-01-01
publisher Wiley
record_format Article
series The Scientific World Journal
spelling doaj-art-c9f7842b26fa4fc4a316bf950b50ceac2025-02-03T07:23:31ZengWileyThe Scientific World Journal2356-61401537-744X2021-01-01202110.1155/2021/37521693752169Antiproliferative Effect of Grammatophyllum speciosum Ethanolic Extract and Its Bioactive Compound on Human Breast Cancer CellsVerisa Chowjarean0Kamala Sadabpod1Cosmeceutical Research, Development and Testing Center, College of Pharmacy, Rangsit University, Lak Hok, Pathum Thani 12000, ThailandDepartment of Pharmacy Practice, College of Pharmacy, Rangsit University, Lak Hok, Pathum Thani 12000, ThailandBackground/Aim. Grammatophyllum speciosum Blume exhibits various promising pharmacological activities. However, its effect on breast cancer has not been determined. Materials and Methods. The antiproliferation effects of the G. speciosum pseudobulb ethanolic extract (GSE) and isovitexin (bioactive constituent) were investigated on the MCF-7 human breast cancer cell line using MTT and colony formation assay. The expression levels of proliferation-regulatory proteins were determined by western blotting. Results. Noncytotoxic concentrations of GSE significantly suppressed the proliferation of MCF-7 cells. Tumor colony formation decreased in both number and size. The level of phosphorylated AKT and β-catenin was suppressed by GSE treatment. Antiproliferation was observed in isovitexin-treated MCF-7 cells in the form of inhibited colony formation and reduced expression of phosphorylated AKT and β-catenin protein. Conclusions. This study demonstrates the novel effect of G. speciosum as an antiproliferative via suppression of the AKT/β-catenin-dependent pathway. This may prompt further investigation of this plant in breast cancer therapy.http://dx.doi.org/10.1155/2021/3752169
spellingShingle Verisa Chowjarean
Kamala Sadabpod
Antiproliferative Effect of Grammatophyllum speciosum Ethanolic Extract and Its Bioactive Compound on Human Breast Cancer Cells
The Scientific World Journal
title Antiproliferative Effect of Grammatophyllum speciosum Ethanolic Extract and Its Bioactive Compound on Human Breast Cancer Cells
title_full Antiproliferative Effect of Grammatophyllum speciosum Ethanolic Extract and Its Bioactive Compound on Human Breast Cancer Cells
title_fullStr Antiproliferative Effect of Grammatophyllum speciosum Ethanolic Extract and Its Bioactive Compound on Human Breast Cancer Cells
title_full_unstemmed Antiproliferative Effect of Grammatophyllum speciosum Ethanolic Extract and Its Bioactive Compound on Human Breast Cancer Cells
title_short Antiproliferative Effect of Grammatophyllum speciosum Ethanolic Extract and Its Bioactive Compound on Human Breast Cancer Cells
title_sort antiproliferative effect of grammatophyllum speciosum ethanolic extract and its bioactive compound on human breast cancer cells
url http://dx.doi.org/10.1155/2021/3752169
work_keys_str_mv AT verisachowjarean antiproliferativeeffectofgrammatophyllumspeciosumethanolicextractanditsbioactivecompoundonhumanbreastcancercells
AT kamalasadabpod antiproliferativeeffectofgrammatophyllumspeciosumethanolicextractanditsbioactivecompoundonhumanbreastcancercells