Investigating formate tolerance mechanisms in Saccharomyces cerevisiae and its application
Current global energy and environmental crisis have spurred efforts towards developing sustainable biotechnological solutions, such as utilizing CO2 and its derivatives as raw materials. Formate is an attractive one-carbon source due to its high solubility and low reduction potential. However, the r...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2023-09-01
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| Series: | Green Carbon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2950155523000071 |
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| author | Kai Wang Yining Liu Zhuoheng Wu Yilu Wu Haoran Bi Yanhui Liu Meng Wang Biqiang Chen Jens Nielsen Zihe Liu Tianwei Tan |
| author_facet | Kai Wang Yining Liu Zhuoheng Wu Yilu Wu Haoran Bi Yanhui Liu Meng Wang Biqiang Chen Jens Nielsen Zihe Liu Tianwei Tan |
| author_sort | Kai Wang |
| collection | DOAJ |
| description | Current global energy and environmental crisis have spurred efforts towards developing sustainable biotechnological solutions, such as utilizing CO2 and its derivatives as raw materials. Formate is an attractive one-carbon source due to its high solubility and low reduction potential. However, the regulatory mechanism of formate metabolism in yeast remains largely unexplored. This study employed adaptive laboratory evolution (ALE) to improve formate tolerance in Saccharomyces cerevisiae and characterized the underlying molecular mechanisms. The evolved strain was applied to produce free fatty acids (FFAs) under high concentration of formate with glucose addition. The results showed that the evolved strain achieved a FFAs titer of 250 mg/L. Overall, this study sheds light on the regulatory mechanism of formate tolerance and provides a platform for future studies under high concentrations of formate. |
| format | Article |
| id | doaj-art-c9ec4fca735041fa8a7106ae4fd9b004 |
| institution | OA Journals |
| issn | 2950-1555 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Green Carbon |
| spelling | doaj-art-c9ec4fca735041fa8a7106ae4fd9b0042025-08-20T01:49:31ZengKeAi Communications Co., Ltd.Green Carbon2950-15552023-09-0111657410.1016/j.greenca.2023.08.003Investigating formate tolerance mechanisms in Saccharomyces cerevisiae and its applicationKai Wang0Yining Liu1Zhuoheng Wu2Yilu Wu3Haoran Bi4Yanhui Liu5Meng Wang6Biqiang Chen7Jens Nielsen8Zihe Liu9Tianwei Tan10National Energy R&D Center for Biorefnery, Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, No. 15 North 3rd Ring Rd East, 100029 Beijing, PR ChinaNational Energy R&D Center for Biorefnery, Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, No. 15 North 3rd Ring Rd East, 100029 Beijing, PR ChinaNational Energy R&D Center for Biorefnery, Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, No. 15 North 3rd Ring Rd East, 100029 Beijing, PR ChinaNational Energy R&D Center for Biorefnery, Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, No. 15 North 3rd Ring Rd East, 100029 Beijing, PR ChinaNational Energy R&D Center for Biorefnery, Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, No. 15 North 3rd Ring Rd East, 100029 Beijing, PR ChinaNational Energy R&D Center for Biorefnery, Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, No. 15 North 3rd Ring Rd East, 100029 Beijing, PR ChinaNational Energy R&D Center for Biorefnery, Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, No. 15 North 3rd Ring Rd East, 100029 Beijing, PR ChinaNational Energy R&D Center for Biorefnery, Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, No. 15 North 3rd Ring Rd East, 100029 Beijing, PR ChinaNational Energy R&D Center for Biorefnery, Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, No. 15 North 3rd Ring Rd East, 100029 Beijing, PR China; Department of Biology and Biological Engineering, Chalmers University of Technology, SE41296 Gothenburg, SwedenNational Energy R&D Center for Biorefnery, Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, No. 15 North 3rd Ring Rd East, 100029 Beijing, PR China; Corresponding authors.National Energy R&D Center for Biorefnery, Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, No. 15 North 3rd Ring Rd East, 100029 Beijing, PR China; Corresponding authors.Current global energy and environmental crisis have spurred efforts towards developing sustainable biotechnological solutions, such as utilizing CO2 and its derivatives as raw materials. Formate is an attractive one-carbon source due to its high solubility and low reduction potential. However, the regulatory mechanism of formate metabolism in yeast remains largely unexplored. This study employed adaptive laboratory evolution (ALE) to improve formate tolerance in Saccharomyces cerevisiae and characterized the underlying molecular mechanisms. The evolved strain was applied to produce free fatty acids (FFAs) under high concentration of formate with glucose addition. The results showed that the evolved strain achieved a FFAs titer of 250 mg/L. Overall, this study sheds light on the regulatory mechanism of formate tolerance and provides a platform for future studies under high concentrations of formate.http://www.sciencedirect.com/science/article/pii/S2950155523000071Formate toleranceAdaptive laboratory evolutionSaccharomyces cerevisiaeFree fatty acids |
| spellingShingle | Kai Wang Yining Liu Zhuoheng Wu Yilu Wu Haoran Bi Yanhui Liu Meng Wang Biqiang Chen Jens Nielsen Zihe Liu Tianwei Tan Investigating formate tolerance mechanisms in Saccharomyces cerevisiae and its application Green Carbon Formate tolerance Adaptive laboratory evolution Saccharomyces cerevisiae Free fatty acids |
| title | Investigating formate tolerance mechanisms in Saccharomyces cerevisiae and its application |
| title_full | Investigating formate tolerance mechanisms in Saccharomyces cerevisiae and its application |
| title_fullStr | Investigating formate tolerance mechanisms in Saccharomyces cerevisiae and its application |
| title_full_unstemmed | Investigating formate tolerance mechanisms in Saccharomyces cerevisiae and its application |
| title_short | Investigating formate tolerance mechanisms in Saccharomyces cerevisiae and its application |
| title_sort | investigating formate tolerance mechanisms in saccharomyces cerevisiae and its application |
| topic | Formate tolerance Adaptive laboratory evolution Saccharomyces cerevisiae Free fatty acids |
| url | http://www.sciencedirect.com/science/article/pii/S2950155523000071 |
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