Long-term preserved bean seeds exhibit high RNA integrity and high germination potential
Abstract Stored RNA plays a key role in seed germination, especially after seeds long-term storage. This study aimed to assess the correlation between germination potential (GP) and RNA integrity in common bean (Phaseolus vulgaris L.) seed accessions stored at -18 °C for 19.25 to 43.75 years and in...
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Nature Portfolio
2025-04-01
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| Online Access: | https://doi.org/10.1038/s41598-024-85030-7 |
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| author | Alisson Ferreira Dantas Tayara Colins Nunes Priscila Grynberg Ana Cristina Miranda Brasileiro Antonieta Nassif Salomão Solange Carvalho Barrios Roveri José Rosana Pereira Vianello Juliano Gomes Pádua Marília de Castro Rodrigues Pappas Marcos Aparecido Gimenes |
| author_facet | Alisson Ferreira Dantas Tayara Colins Nunes Priscila Grynberg Ana Cristina Miranda Brasileiro Antonieta Nassif Salomão Solange Carvalho Barrios Roveri José Rosana Pereira Vianello Juliano Gomes Pádua Marília de Castro Rodrigues Pappas Marcos Aparecido Gimenes |
| author_sort | Alisson Ferreira Dantas |
| collection | DOAJ |
| description | Abstract Stored RNA plays a key role in seed germination, especially after seeds long-term storage. This study aimed to assess the correlation between germination potential (GP) and RNA integrity in common bean (Phaseolus vulgaris L.) seed accessions stored at -18 °C for 19.25 to 43.75 years and in seeds subjected to artificial aging at high temperature (42 °C) and relative humidity (100%). The GP of long-term preserved and artificially aged seed lots ranged from 3 to 100% and 38–87%, respectively. RNA integrity was evaluated using the RNA Integrity Number (RIN, Agilent Bioanalyzer software). RIN data suggested that: (1) Sample sizes for RIN analysis may need to vary based on the GP of the seeds; (2) RIN has the potential to predict physiological quality, especially in seeds with high GP; (3) RIN values by itself may not accurately reflect the rate of RNA degradation over time, necessitating comparison with a control to determine ΔRIN (the difference between the RIN values of the sample and the control); (4) ΔRIN has a significant positive correlation with GP. These findings highlight the potential of RNA integrity in seeds as a molecular marker for developing tests that complement germination tests. |
| format | Article |
| id | doaj-art-c9ebec03c43241dfb0a90e7275bd28eb |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-c9ebec03c43241dfb0a90e7275bd28eb2025-08-20T02:28:03ZengNature PortfolioScientific Reports2045-23222025-04-0115111210.1038/s41598-024-85030-7Long-term preserved bean seeds exhibit high RNA integrity and high germination potentialAlisson Ferreira Dantas0Tayara Colins Nunes1Priscila Grynberg2Ana Cristina Miranda Brasileiro3Antonieta Nassif Salomão4Solange Carvalho Barrios Roveri José5Rosana Pereira Vianello6Juliano Gomes Pádua7Marília de Castro Rodrigues Pappas8Marcos Aparecido Gimenes9National Council for Scientific and Technological DevelopmentFederal District Research Support FoundationEmbrapa Genetic Resources & BiotechnologyEmbrapa Genetic Resources & BiotechnologyEmbrapa Genetic Resources & BiotechnologyEmbrapa Genetic Resources & BiotechnologyEmbrapa Rice & BeansEmbrapa Genetic Resources & BiotechnologyEmbrapa Genetic Resources & BiotechnologyEmbrapa Genetic Resources & BiotechnologyAbstract Stored RNA plays a key role in seed germination, especially after seeds long-term storage. This study aimed to assess the correlation between germination potential (GP) and RNA integrity in common bean (Phaseolus vulgaris L.) seed accessions stored at -18 °C for 19.25 to 43.75 years and in seeds subjected to artificial aging at high temperature (42 °C) and relative humidity (100%). The GP of long-term preserved and artificially aged seed lots ranged from 3 to 100% and 38–87%, respectively. RNA integrity was evaluated using the RNA Integrity Number (RIN, Agilent Bioanalyzer software). RIN data suggested that: (1) Sample sizes for RIN analysis may need to vary based on the GP of the seeds; (2) RIN has the potential to predict physiological quality, especially in seeds with high GP; (3) RIN values by itself may not accurately reflect the rate of RNA degradation over time, necessitating comparison with a control to determine ΔRIN (the difference between the RIN values of the sample and the control); (4) ΔRIN has a significant positive correlation with GP. These findings highlight the potential of RNA integrity in seeds as a molecular marker for developing tests that complement germination tests.https://doi.org/10.1038/s41598-024-85030-7Aged seedsPhaseolus vulgaris L.RNA integrity numberSeed conservation |
| spellingShingle | Alisson Ferreira Dantas Tayara Colins Nunes Priscila Grynberg Ana Cristina Miranda Brasileiro Antonieta Nassif Salomão Solange Carvalho Barrios Roveri José Rosana Pereira Vianello Juliano Gomes Pádua Marília de Castro Rodrigues Pappas Marcos Aparecido Gimenes Long-term preserved bean seeds exhibit high RNA integrity and high germination potential Scientific Reports Aged seeds Phaseolus vulgaris L. RNA integrity number Seed conservation |
| title | Long-term preserved bean seeds exhibit high RNA integrity and high germination potential |
| title_full | Long-term preserved bean seeds exhibit high RNA integrity and high germination potential |
| title_fullStr | Long-term preserved bean seeds exhibit high RNA integrity and high germination potential |
| title_full_unstemmed | Long-term preserved bean seeds exhibit high RNA integrity and high germination potential |
| title_short | Long-term preserved bean seeds exhibit high RNA integrity and high germination potential |
| title_sort | long term preserved bean seeds exhibit high rna integrity and high germination potential |
| topic | Aged seeds Phaseolus vulgaris L. RNA integrity number Seed conservation |
| url | https://doi.org/10.1038/s41598-024-85030-7 |
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