METHODICAL APPROACH TO ASSESSMENT OF THE MORPHOLOGICAL CHANGES IN ACUPUNCTURE OF TONGUE
Changes occurring in acupuncture and sublingually administration of medicines also have a morphological basis, which can be objectively assessed. Objective: Refine of the methodology for evaluating morphological changes associated with tongue acupuncture. Material and methods: Selection of experime...
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| Language: | English |
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Trakia University
2019-11-01
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| Series: | Trakia Journal of Sciences |
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| Online Access: | http://tru.uni-sz.bg/tsj/Volume%2017,%202019,%20Supplement%202,%20Series%20Biomedical%20Sciences/16_N.Pirovski.pdf |
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| author | N. Pirovski N. Dimitrov D. Atanasova |
| author_facet | N. Pirovski N. Dimitrov D. Atanasova |
| author_sort | N. Pirovski |
| collection | DOAJ |
| description | Changes occurring in acupuncture and sublingually administration of medicines also have a morphological basis, which can be objectively assessed. Objective: Refine of the methodology for evaluating morphological changes associated with tongue acupuncture. Material and methods: Selection
of experimental animals and appropriate protocol for material collection, fixation, staining and counting of selected morphological structures and elements. Results: The samples fixed with formalin 10% were fixed well, the consistency of the tissues remained hard and the cuts were obtained well in all
thicknesses (from 5 to 15 μm). Cuts from 5 to 7 μm are suitable for visualizing the structure of the tongue. The sagittal cuts provide the largest area for single-cut exploration, as well as are more suitable than others to track the acupuncture channel in the chosen for our study point. Conclusions: The study
can be carried out in rats, but if possible, it is preferable to take the material from pigs. The use of formalin 10% gives a better texture to the material and facilitates the cutting with the microtome. It is necessary to use a variety of colorings due to the wide range of structures that are involved with the
acupuncture needle tract. |
| format | Article |
| id | doaj-art-c9e48fd11c5842ca86f699c5dde90fc0 |
| institution | DOAJ |
| issn | 1313-3551 |
| language | English |
| publishDate | 2019-11-01 |
| publisher | Trakia University |
| record_format | Article |
| series | Trakia Journal of Sciences |
| spelling | doaj-art-c9e48fd11c5842ca86f699c5dde90fc02025-08-20T02:55:02ZengTrakia UniversityTrakia Journal of Sciences1313-35512019-11-0117Suppl. 2737510.15547/tjs.2019.s.02.016METHODICAL APPROACH TO ASSESSMENT OF THE MORPHOLOGICAL CHANGES IN ACUPUNCTURE OF TONGUEN. PirovskiN. DimitrovD. AtanasovaChanges occurring in acupuncture and sublingually administration of medicines also have a morphological basis, which can be objectively assessed. Objective: Refine of the methodology for evaluating morphological changes associated with tongue acupuncture. Material and methods: Selection of experimental animals and appropriate protocol for material collection, fixation, staining and counting of selected morphological structures and elements. Results: The samples fixed with formalin 10% were fixed well, the consistency of the tissues remained hard and the cuts were obtained well in all thicknesses (from 5 to 15 μm). Cuts from 5 to 7 μm are suitable for visualizing the structure of the tongue. The sagittal cuts provide the largest area for single-cut exploration, as well as are more suitable than others to track the acupuncture channel in the chosen for our study point. Conclusions: The study can be carried out in rats, but if possible, it is preferable to take the material from pigs. The use of formalin 10% gives a better texture to the material and facilitates the cutting with the microtome. It is necessary to use a variety of colorings due to the wide range of structures that are involved with the acupuncture needle tract.http://tru.uni-sz.bg/tsj/Volume%2017,%202019,%20Supplement%202,%20Series%20Biomedical%20Sciences/16_N.Pirovski.pdftonguemorphologymethodologyacupuncture |
| spellingShingle | N. Pirovski N. Dimitrov D. Atanasova METHODICAL APPROACH TO ASSESSMENT OF THE MORPHOLOGICAL CHANGES IN ACUPUNCTURE OF TONGUE Trakia Journal of Sciences tongue morphology methodology acupuncture |
| title | METHODICAL APPROACH TO ASSESSMENT OF THE MORPHOLOGICAL CHANGES IN ACUPUNCTURE OF TONGUE |
| title_full | METHODICAL APPROACH TO ASSESSMENT OF THE MORPHOLOGICAL CHANGES IN ACUPUNCTURE OF TONGUE |
| title_fullStr | METHODICAL APPROACH TO ASSESSMENT OF THE MORPHOLOGICAL CHANGES IN ACUPUNCTURE OF TONGUE |
| title_full_unstemmed | METHODICAL APPROACH TO ASSESSMENT OF THE MORPHOLOGICAL CHANGES IN ACUPUNCTURE OF TONGUE |
| title_short | METHODICAL APPROACH TO ASSESSMENT OF THE MORPHOLOGICAL CHANGES IN ACUPUNCTURE OF TONGUE |
| title_sort | methodical approach to assessment of the morphological changes in acupuncture of tongue |
| topic | tongue morphology methodology acupuncture |
| url | http://tru.uni-sz.bg/tsj/Volume%2017,%202019,%20Supplement%202,%20Series%20Biomedical%20Sciences/16_N.Pirovski.pdf |
| work_keys_str_mv | AT npirovski methodicalapproachtoassessmentofthemorphologicalchangesinacupunctureoftongue AT ndimitrov methodicalapproachtoassessmentofthemorphologicalchangesinacupunctureoftongue AT datanasova methodicalapproachtoassessmentofthemorphologicalchangesinacupunctureoftongue |