Analysis of immune cell activation in patients with diabetes foot ulcer from the perspective of single cell
Abstract Background Diabetes mellitus (DM) can cause severe complications, including diabetic foot ulcers (DFU). There is a significant gap in understanding the single-cell ecological atlas of DM and DFU tissues. Methods Single-cell RNA sequencing data were used to create a detailed single-cell ecol...
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| Format: | Article |
| Language: | English |
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BMC
2024-12-01
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| Series: | European Journal of Medical Research |
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| Online Access: | https://doi.org/10.1186/s40001-024-02179-7 |
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| author | Lehoanganh Vu Fei Xu Ting Li Qikai Hua Xiaocong Kuang Yongqiang Jiang Yanfei Liang Xing Niu Yixuan Chen Chengyu Huang Weiliang Mo Kejian Wang Kaihua Tang Jianwen Mo Ke-Er Lu Yan Mo Steven Mo Dengfeng Yang Jinmin Zhao |
| author_facet | Lehoanganh Vu Fei Xu Ting Li Qikai Hua Xiaocong Kuang Yongqiang Jiang Yanfei Liang Xing Niu Yixuan Chen Chengyu Huang Weiliang Mo Kejian Wang Kaihua Tang Jianwen Mo Ke-Er Lu Yan Mo Steven Mo Dengfeng Yang Jinmin Zhao |
| author_sort | Lehoanganh Vu |
| collection | DOAJ |
| description | Abstract Background Diabetes mellitus (DM) can cause severe complications, including diabetic foot ulcers (DFU). There is a significant gap in understanding the single-cell ecological atlas of DM and DFU tissues. Methods Single-cell RNA sequencing data were used to create a detailed single-cell ecological landscape of DM and DFU. Enrichment analysis identified pathways involved in cellular subpopulations, and pseudo-time analysis inferred cell development processes. A gene regulatory network explored the role of transcription factors in DFU progression, and a potential herbal drug-target gene interaction network was constructed. Results In the DFU group, immune cells were activated, with notable changes in several subpopulations. ATP5E was significantly overexpressed in Naive T cells, fibroblasts, endothelial cells, and CD8+ T cells in DM patients. Specific immune cell subsets, such as Naive T_RGCC, CTL_TYROBP_CL4, Mac_SLC40A1, and M1_CCL3L1, likely contribute to DFU formation through overactivation and proliferation, leading to tissue damage and ulcer exacerbation. Key genes TPP1, TLR4, and RIPK2 were identified, and 88 active ingredients in the herbal drug-target network showed strong correlations with these targets. Herbs like Angelica dahurica, Angelica sinensis, Boswellia carterii, liquorice, myrrh, and Semen armeniacae amarae were included. Conclusions This study offers insights into DM and DFU cytology. T cells in DFU are activated, attacking normal tissues and worsening tissue damage. The ATP5E gene may be related to the ecological remodeling of DM, and TPP1, TLR4, and RIPK2 are potential targets for DFU treatment. |
| format | Article |
| id | doaj-art-c9e259812eb54077b2e55f62db71e2a4 |
| institution | OA Journals |
| issn | 2047-783X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | European Journal of Medical Research |
| spelling | doaj-art-c9e259812eb54077b2e55f62db71e2a42025-08-20T02:32:01ZengBMCEuropean Journal of Medical Research2047-783X2024-12-0129111810.1186/s40001-024-02179-7Analysis of immune cell activation in patients with diabetes foot ulcer from the perspective of single cellLehoanganh Vu0Fei Xu1Ting Li2Qikai Hua3Xiaocong Kuang4Yongqiang Jiang5Yanfei Liang6Xing Niu7Yixuan Chen8Chengyu Huang9Weiliang Mo10Kejian Wang11Kaihua Tang12Jianwen Mo13Ke-Er Lu14Yan Mo15Steven Mo16Dengfeng Yang17Jinmin Zhao18Department of Bone and Joint Surgery, The First Affiliated Hospital of Guangxi Medical UniversitySystems Biology Research Center, Biology Institute, Guangxi Academy of SciencesSystems Biology Research Center, Biology Institute, Guangxi Academy of SciencesDepartment of Bone and Joint Surgery, The First Affiliated Hospital of Guangxi Medical UniversityDepartment of Physiology and Pathophysiology, Yulin Campus of Guangxi Medical UniversitySystems Biology Research Center, Biology Institute, Guangxi Academy of SciencesSystems Biology Research Center, Biology Institute, Guangxi Academy of SciencesSystems Biology Research Center, Biology Institute, Guangxi Academy of SciencesSystems Biology Research Center, Biology Institute, Guangxi Academy of SciencesSystems Biology Research Center, Biology Institute, Guangxi Academy of SciencesSystems Biology Research Center, Biology Institute, Guangxi Academy of SciencesSystems Biology Research Center, Biology Institute, Guangxi Academy of SciencesSystems Biology Research Center, Biology Institute, Guangxi Academy of SciencesSystems Biology Research Center, Biology Institute, Guangxi Academy of SciencesSystems Biology Research Center, Biology Institute, Guangxi Academy of SciencesDepartment of Pathology, Yulin Campus of Guangxi Medical UniversitySystems Biology Research Center, Biology Institute, Guangxi Academy of SciencesSystems Biology Research Center, Biology Institute, Guangxi Academy of SciencesDepartment of Bone and Joint Surgery, The First Affiliated Hospital of Guangxi Medical UniversityAbstract Background Diabetes mellitus (DM) can cause severe complications, including diabetic foot ulcers (DFU). There is a significant gap in understanding the single-cell ecological atlas of DM and DFU tissues. Methods Single-cell RNA sequencing data were used to create a detailed single-cell ecological landscape of DM and DFU. Enrichment analysis identified pathways involved in cellular subpopulations, and pseudo-time analysis inferred cell development processes. A gene regulatory network explored the role of transcription factors in DFU progression, and a potential herbal drug-target gene interaction network was constructed. Results In the DFU group, immune cells were activated, with notable changes in several subpopulations. ATP5E was significantly overexpressed in Naive T cells, fibroblasts, endothelial cells, and CD8+ T cells in DM patients. Specific immune cell subsets, such as Naive T_RGCC, CTL_TYROBP_CL4, Mac_SLC40A1, and M1_CCL3L1, likely contribute to DFU formation through overactivation and proliferation, leading to tissue damage and ulcer exacerbation. Key genes TPP1, TLR4, and RIPK2 were identified, and 88 active ingredients in the herbal drug-target network showed strong correlations with these targets. Herbs like Angelica dahurica, Angelica sinensis, Boswellia carterii, liquorice, myrrh, and Semen armeniacae amarae were included. Conclusions This study offers insights into DM and DFU cytology. T cells in DFU are activated, attacking normal tissues and worsening tissue damage. The ATP5E gene may be related to the ecological remodeling of DM, and TPP1, TLR4, and RIPK2 are potential targets for DFU treatment.https://doi.org/10.1186/s40001-024-02179-7Diabetes mellitusDFUSingle-cell RNA sequencingApoptosisImmune activationDrug targets |
| spellingShingle | Lehoanganh Vu Fei Xu Ting Li Qikai Hua Xiaocong Kuang Yongqiang Jiang Yanfei Liang Xing Niu Yixuan Chen Chengyu Huang Weiliang Mo Kejian Wang Kaihua Tang Jianwen Mo Ke-Er Lu Yan Mo Steven Mo Dengfeng Yang Jinmin Zhao Analysis of immune cell activation in patients with diabetes foot ulcer from the perspective of single cell European Journal of Medical Research Diabetes mellitus DFU Single-cell RNA sequencing Apoptosis Immune activation Drug targets |
| title | Analysis of immune cell activation in patients with diabetes foot ulcer from the perspective of single cell |
| title_full | Analysis of immune cell activation in patients with diabetes foot ulcer from the perspective of single cell |
| title_fullStr | Analysis of immune cell activation in patients with diabetes foot ulcer from the perspective of single cell |
| title_full_unstemmed | Analysis of immune cell activation in patients with diabetes foot ulcer from the perspective of single cell |
| title_short | Analysis of immune cell activation in patients with diabetes foot ulcer from the perspective of single cell |
| title_sort | analysis of immune cell activation in patients with diabetes foot ulcer from the perspective of single cell |
| topic | Diabetes mellitus DFU Single-cell RNA sequencing Apoptosis Immune activation Drug targets |
| url | https://doi.org/10.1186/s40001-024-02179-7 |
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