Persistent Inflammation in the CNS during Chronic EAE Despite Local Absence of IL-17 Production
Experimental autoimmune encephalomyelitis (EAE) is an artificially induced demyelination of the central nervous system (CNS) that resembles multiple sclerosis in its clinical, histopathological, and immunological features. Activated Th1 and Th17 cells are thought to be the main immunological players...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2013/519627 |
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| author | Sofia Fernanda Gonçalves Zorzella-Pezavento Fernanda Chiuso-Minicucci Thais Graziela Donegá França Larissa Lumi Watanabe Ishikawa Larissa Camargo da Rosa Camila Marques Maura Rosane Valerio Ikoma Alexandrina Sartori |
| author_facet | Sofia Fernanda Gonçalves Zorzella-Pezavento Fernanda Chiuso-Minicucci Thais Graziela Donegá França Larissa Lumi Watanabe Ishikawa Larissa Camargo da Rosa Camila Marques Maura Rosane Valerio Ikoma Alexandrina Sartori |
| author_sort | Sofia Fernanda Gonçalves Zorzella-Pezavento |
| collection | DOAJ |
| description | Experimental autoimmune encephalomyelitis (EAE) is an artificially induced demyelination of the central nervous system (CNS) that resembles multiple sclerosis in its clinical, histopathological, and immunological features. Activated Th1 and Th17 cells are thought to be the main immunological players during EAE development. This study was designed to evaluate peripheral and local contribution of IL-17 to acute and chronic EAE stages. C57BL/6 mice were immunized with MOG plus complete Freund’s adjuvant followed by pertussis toxin. Mice presented an initial acute phase characterized by accentuated weight loss and high clinical score, followed by a partial recovery when the animals reached normal body weight and smaller clinical scores. Spleen cells stimulated with MOG produced significantly higher levels of IFN-γ during the acute period whereas similar IL-17 levels were produced during both disease stages. CNS-infiltrating cells stimulated with MOG produced similar amounts of IFN-γ but, IL-17 was produced only at the acute phase of EAE. The percentage of Foxp3+ Treg cells, at the spleen and CNS, was elevated during both phases. The degree of inflammation was similar at both disease stages. Partial clinical recovery observed during chronic EAE was associated with no IL-17 production and presence of Foxp3+ Treg cells in the CNS. |
| format | Article |
| id | doaj-art-c9e13e4b7b444de5af5314cab1f060cd |
| institution | DOAJ |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-c9e13e4b7b444de5af5314cab1f060cd2025-08-20T03:23:59ZengWileyMediators of Inflammation0962-93511466-18612013-01-01201310.1155/2013/519627519627Persistent Inflammation in the CNS during Chronic EAE Despite Local Absence of IL-17 ProductionSofia Fernanda Gonçalves Zorzella-Pezavento0Fernanda Chiuso-Minicucci1Thais Graziela Donegá França2Larissa Lumi Watanabe Ishikawa3Larissa Camargo da Rosa4Camila Marques5Maura Rosane Valerio Ikoma6Alexandrina Sartori7Department of Microbiology and Immunology, Biosciences Institute, Universidade Estadual Paulista (UNESP), 18618-070 Botucatu, SP, BrazilDepartment of Microbiology and Immunology, Biosciences Institute, Universidade Estadual Paulista (UNESP), 18618-070 Botucatu, SP, BrazilDepartment of Microbiology and Immunology, Biosciences Institute, Universidade Estadual Paulista (UNESP), 18618-070 Botucatu, SP, BrazilDepartment of Microbiology and Immunology, Biosciences Institute, Universidade Estadual Paulista (UNESP), 18618-070 Botucatu, SP, BrazilDepartment of Microbiology and Immunology, Biosciences Institute, Universidade Estadual Paulista (UNESP), 18618-070 Botucatu, SP, BrazilLaboratório de Citometria de Fluxo-Fundação Dr. Amaral Carvalho, Jaú, SP, BrazilLaboratório de Citometria de Fluxo-Fundação Dr. Amaral Carvalho, Jaú, SP, BrazilDepartment of Microbiology and Immunology, Biosciences Institute, Universidade Estadual Paulista (UNESP), 18618-070 Botucatu, SP, BrazilExperimental autoimmune encephalomyelitis (EAE) is an artificially induced demyelination of the central nervous system (CNS) that resembles multiple sclerosis in its clinical, histopathological, and immunological features. Activated Th1 and Th17 cells are thought to be the main immunological players during EAE development. This study was designed to evaluate peripheral and local contribution of IL-17 to acute and chronic EAE stages. C57BL/6 mice were immunized with MOG plus complete Freund’s adjuvant followed by pertussis toxin. Mice presented an initial acute phase characterized by accentuated weight loss and high clinical score, followed by a partial recovery when the animals reached normal body weight and smaller clinical scores. Spleen cells stimulated with MOG produced significantly higher levels of IFN-γ during the acute period whereas similar IL-17 levels were produced during both disease stages. CNS-infiltrating cells stimulated with MOG produced similar amounts of IFN-γ but, IL-17 was produced only at the acute phase of EAE. The percentage of Foxp3+ Treg cells, at the spleen and CNS, was elevated during both phases. The degree of inflammation was similar at both disease stages. Partial clinical recovery observed during chronic EAE was associated with no IL-17 production and presence of Foxp3+ Treg cells in the CNS.http://dx.doi.org/10.1155/2013/519627 |
| spellingShingle | Sofia Fernanda Gonçalves Zorzella-Pezavento Fernanda Chiuso-Minicucci Thais Graziela Donegá França Larissa Lumi Watanabe Ishikawa Larissa Camargo da Rosa Camila Marques Maura Rosane Valerio Ikoma Alexandrina Sartori Persistent Inflammation in the CNS during Chronic EAE Despite Local Absence of IL-17 Production Mediators of Inflammation |
| title | Persistent Inflammation in the CNS during Chronic EAE Despite Local Absence of IL-17 Production |
| title_full | Persistent Inflammation in the CNS during Chronic EAE Despite Local Absence of IL-17 Production |
| title_fullStr | Persistent Inflammation in the CNS during Chronic EAE Despite Local Absence of IL-17 Production |
| title_full_unstemmed | Persistent Inflammation in the CNS during Chronic EAE Despite Local Absence of IL-17 Production |
| title_short | Persistent Inflammation in the CNS during Chronic EAE Despite Local Absence of IL-17 Production |
| title_sort | persistent inflammation in the cns during chronic eae despite local absence of il 17 production |
| url | http://dx.doi.org/10.1155/2013/519627 |
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