Diesel Engine Exhaust Initiates a Sequence of Pulmonary and Cardiovascular Effects in Rats
This study was designed to determine the sequence of events leading to cardiopulmonary effects following acute inhalation of diesel engine exhaust in rats. Rats were exposed for 2 h to diesel engine exhaust (1.9 mg/m3), and biological parameters related to antioxidant defense, inflammation, and proc...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2010-01-01
|
| Series: | Journal of Toxicology |
| Online Access: | http://dx.doi.org/10.1155/2010/206057 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850174226859819008 |
|---|---|
| author | Ingeborg M. Kooter Miriam E. Gerlofs-Nijland A. John F. Boere Daan L. A. C. Leseman Paul H. B. Fokkens Henri M. H. Spronk Kim Frederix Hugo ten Cate Ad M. Knaapen Hendrik J. Vreman Flemming R. Cassee |
| author_facet | Ingeborg M. Kooter Miriam E. Gerlofs-Nijland A. John F. Boere Daan L. A. C. Leseman Paul H. B. Fokkens Henri M. H. Spronk Kim Frederix Hugo ten Cate Ad M. Knaapen Hendrik J. Vreman Flemming R. Cassee |
| author_sort | Ingeborg M. Kooter |
| collection | DOAJ |
| description | This study was designed to determine the sequence of events leading to cardiopulmonary effects following acute inhalation of diesel engine exhaust in rats. Rats were exposed for 2 h to diesel engine exhaust (1.9 mg/m3), and biological parameters related to antioxidant defense, inflammation, and procoagulation were examined after 4, 18, 24, 48, and 72 h.
This in vivo inhalation study showed a pulmonary anti-oxidant response (an increased activity of the anti-oxidant enzymes glutathione peroxidase and superoxide dismutase and an increase in heme oxygenase-1 protein, heme oxygenase activity, and uric acid) which precedes the inflammatory response (an increase in IL-6 and TNF-α). In addition, increased plasma thrombogenicity and immediate anti-oxidant defense gene expression in aorta tissue shortly after the exposure might suggest direct translocation of diesel engine exhaust components to the vasculature but mediation by other pathways cannot be ruled out. This study therefore shows that different stages in oxidative stress are not only affected by dose increments but are also time dependent. |
| format | Article |
| id | doaj-art-c9dde9cda6e14617a4d0d861124eb3ff |
| institution | OA Journals |
| issn | 1687-8191 1687-8205 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Toxicology |
| spelling | doaj-art-c9dde9cda6e14617a4d0d861124eb3ff2025-08-20T02:19:42ZengWileyJournal of Toxicology1687-81911687-82052010-01-01201010.1155/2010/206057206057Diesel Engine Exhaust Initiates a Sequence of Pulmonary and Cardiovascular Effects in RatsIngeborg M. Kooter0Miriam E. Gerlofs-Nijland1A. John F. Boere2Daan L. A. C. Leseman3Paul H. B. Fokkens4Henri M. H. Spronk5Kim Frederix6Hugo ten Cate7Ad M. Knaapen8Hendrik J. Vreman9Flemming R. Cassee10Department of Environment, Health and Safety, TNO Built, Environment and Geosciences, Princetonlaan 6, 3584 CB Utrecht, The NetherlandsCentre for Environmental Health Research, National Institute for Public Health and the Environment, 3720 BA Bilthoven, The NetherlandsCentre for Environmental Health Research, National Institute for Public Health and the Environment, 3720 BA Bilthoven, The NetherlandsCentre for Environmental Health Research, National Institute for Public Health and the Environment, 3720 BA Bilthoven, The NetherlandsCentre for Environmental Health Research, National Institute for Public Health and the Environment, 3720 BA Bilthoven, The NetherlandsDepartment of Internal Medicine, Laboratory of Clinical Thrombosis and Haemostasis, Cardiovascular Research Institute Maastricht, Maastricht University Medical Center, 6229 ER Maastricht, The NetherlandsDepartment of Internal Medicine, Laboratory of Clinical Thrombosis and Haemostasis, Cardiovascular Research Institute Maastricht, Maastricht University Medical Center, 6229 ER Maastricht, The NetherlandsDepartment of Internal Medicine, Laboratory of Clinical Thrombosis and Haemostasis, Cardiovascular Research Institute Maastricht, Maastricht University Medical Center, 6229 ER Maastricht, The NetherlandsDepartment of Health Risk Analysis and Toxicology, Nutrition and Toxicology Research Institute (NUTRIM), Maastricht University, 6229 ER Maastricht, The NetherlandsDivision of Neonatal & Developmental Medicine, Department of Pediatrics, Stanford University Medical Center, Stanford, CA 94305-5208, USACentre for Environmental Health Research, National Institute for Public Health and the Environment, 3720 BA Bilthoven, The NetherlandsThis study was designed to determine the sequence of events leading to cardiopulmonary effects following acute inhalation of diesel engine exhaust in rats. Rats were exposed for 2 h to diesel engine exhaust (1.9 mg/m3), and biological parameters related to antioxidant defense, inflammation, and procoagulation were examined after 4, 18, 24, 48, and 72 h. This in vivo inhalation study showed a pulmonary anti-oxidant response (an increased activity of the anti-oxidant enzymes glutathione peroxidase and superoxide dismutase and an increase in heme oxygenase-1 protein, heme oxygenase activity, and uric acid) which precedes the inflammatory response (an increase in IL-6 and TNF-α). In addition, increased plasma thrombogenicity and immediate anti-oxidant defense gene expression in aorta tissue shortly after the exposure might suggest direct translocation of diesel engine exhaust components to the vasculature but mediation by other pathways cannot be ruled out. This study therefore shows that different stages in oxidative stress are not only affected by dose increments but are also time dependent.http://dx.doi.org/10.1155/2010/206057 |
| spellingShingle | Ingeborg M. Kooter Miriam E. Gerlofs-Nijland A. John F. Boere Daan L. A. C. Leseman Paul H. B. Fokkens Henri M. H. Spronk Kim Frederix Hugo ten Cate Ad M. Knaapen Hendrik J. Vreman Flemming R. Cassee Diesel Engine Exhaust Initiates a Sequence of Pulmonary and Cardiovascular Effects in Rats Journal of Toxicology |
| title | Diesel Engine Exhaust Initiates a Sequence of Pulmonary and Cardiovascular Effects in Rats |
| title_full | Diesel Engine Exhaust Initiates a Sequence of Pulmonary and Cardiovascular Effects in Rats |
| title_fullStr | Diesel Engine Exhaust Initiates a Sequence of Pulmonary and Cardiovascular Effects in Rats |
| title_full_unstemmed | Diesel Engine Exhaust Initiates a Sequence of Pulmonary and Cardiovascular Effects in Rats |
| title_short | Diesel Engine Exhaust Initiates a Sequence of Pulmonary and Cardiovascular Effects in Rats |
| title_sort | diesel engine exhaust initiates a sequence of pulmonary and cardiovascular effects in rats |
| url | http://dx.doi.org/10.1155/2010/206057 |
| work_keys_str_mv | AT ingeborgmkooter dieselengineexhaustinitiatesasequenceofpulmonaryandcardiovasculareffectsinrats AT miriamegerlofsnijland dieselengineexhaustinitiatesasequenceofpulmonaryandcardiovasculareffectsinrats AT ajohnfboere dieselengineexhaustinitiatesasequenceofpulmonaryandcardiovasculareffectsinrats AT daanlacleseman dieselengineexhaustinitiatesasequenceofpulmonaryandcardiovasculareffectsinrats AT paulhbfokkens dieselengineexhaustinitiatesasequenceofpulmonaryandcardiovasculareffectsinrats AT henrimhspronk dieselengineexhaustinitiatesasequenceofpulmonaryandcardiovasculareffectsinrats AT kimfrederix dieselengineexhaustinitiatesasequenceofpulmonaryandcardiovasculareffectsinrats AT hugotencate dieselengineexhaustinitiatesasequenceofpulmonaryandcardiovasculareffectsinrats AT admknaapen dieselengineexhaustinitiatesasequenceofpulmonaryandcardiovasculareffectsinrats AT hendrikjvreman dieselengineexhaustinitiatesasequenceofpulmonaryandcardiovasculareffectsinrats AT flemmingrcassee dieselengineexhaustinitiatesasequenceofpulmonaryandcardiovasculareffectsinrats |