STIRRED BIOREACTOR FOR THE ROBUSTNESS PRODUCTION OF RECOMBINANT GST.VP28 IN FED-BATCH CULTIVATION OF ESCHERICHIA COLI
Escherichia coli is the most popular platform for the production of recombinant proteins as vaccine candidates. One important factor that may influence the quantity and quality of the expressed proteins using the bacterial host is a bioreactor. Thus, this study was aimed at comparing the influence o...
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Alma Mater Publishing House "Vasile Alecsandri" University of Bacau
2015-10-01
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| Series: | Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry |
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| Online Access: | http://pubs.ub.ro/?pg=revues&rev=cscc6&num=201503&vol=3&aid=4301 |
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| author | MUHAMAD ALI ISMAINI SULAIMAN N. DEPAMEDE BAGUS D. H. SETYONO ALIS MUKHLIS MUHAMAD AMIN MOHAMMAD ASHARI |
| author_facet | MUHAMAD ALI ISMAINI SULAIMAN N. DEPAMEDE BAGUS D. H. SETYONO ALIS MUKHLIS MUHAMAD AMIN MOHAMMAD ASHARI |
| author_sort | MUHAMAD ALI |
| collection | DOAJ |
| description | Escherichia coli is the most popular platform for the production of recombinant proteins as vaccine candidates. One important factor that may influence the quantity and quality of the expressed proteins using the bacterial host is a bioreactor. Thus, this study was aimed at comparing the influence of two different bioreactors, conventional (Sakaguchi flask) and stirred bioreactors on the growth of E. coli BL21 as a host cell and production of GST.VP28 recombinant protein in the host. The result showed that total wet biomass of E. coli BL21 harvested from the stirred bioreactor was significantly higher, compared to the total biomass from the conventional bioreactors. In addition, SDS-PAGE results also indicated that the amount of the GST.VP28 recombinant protein collected from the stirred bioreactor was higher than the quantity of recombinant protein from E. coli cultured in the conventional bioreactor. Furthermore, the stirred bioreactor also produced larger soluble fraction and lower inclusion body. This result suggested that the stirred bioreactor is very suitable for improving the production of recombinant protein using E. coli. |
| format | Article |
| id | doaj-art-c9da2eec99a847c8b2c544202f5f7b06 |
| institution | DOAJ |
| issn | 1582-540X 1582-540X |
| language | English |
| publishDate | 2015-10-01 |
| publisher | Alma Mater Publishing House "Vasile Alecsandri" University of Bacau |
| record_format | Article |
| series | Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry |
| spelling | doaj-art-c9da2eec99a847c8b2c544202f5f7b062025-08-20T03:09:42ZengAlma Mater Publishing House "Vasile Alecsandri" University of BacauScientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry1582-540X1582-540X2015-10-01163245252STIRRED BIOREACTOR FOR THE ROBUSTNESS PRODUCTION OF RECOMBINANT GST.VP28 IN FED-BATCH CULTIVATION OF ESCHERICHIA COLIMUHAMAD ALI 0ISMAINI1SULAIMAN N. DEPAMEDE2BAGUS D. H. SETYONO3ALIS MUKHLIS4MUHAMAD AMIN5MOHAMMAD ASHARI6Mataram University, Faculty of Animal Sciences, Jl. Majapahit No. 62 Mataram, 83125, IndonesiaMataram University, Faculty of Animal Sciences, Jl. Majapahit No. 62 Mataram, 83125, IndonesiaMataram University, Faculty of Animal Sciences, Jl. Majapahit No. 62 Mataram, 83125, IndonesiaMataram University, Fisheries Study Program, Jl. Majapahit No. 62 Mataram, 83125, IndonesiaMataram University, Fisheries Study Program, Jl. Majapahit No. 62 Mataram, 83125, IndonesiaUniversity of 45 Mataram, Fisheries Faculty, Mataram, 83125, Indonesia Mataram University, Faculty of Animal Sciences, Jl. Majapahit No. 62 Mataram, 83125, IndonesiaEscherichia coli is the most popular platform for the production of recombinant proteins as vaccine candidates. One important factor that may influence the quantity and quality of the expressed proteins using the bacterial host is a bioreactor. Thus, this study was aimed at comparing the influence of two different bioreactors, conventional (Sakaguchi flask) and stirred bioreactors on the growth of E. coli BL21 as a host cell and production of GST.VP28 recombinant protein in the host. The result showed that total wet biomass of E. coli BL21 harvested from the stirred bioreactor was significantly higher, compared to the total biomass from the conventional bioreactors. In addition, SDS-PAGE results also indicated that the amount of the GST.VP28 recombinant protein collected from the stirred bioreactor was higher than the quantity of recombinant protein from E. coli cultured in the conventional bioreactor. Furthermore, the stirred bioreactor also produced larger soluble fraction and lower inclusion body. This result suggested that the stirred bioreactor is very suitable for improving the production of recombinant protein using E. coli.http://pubs.ub.ro/?pg=revues&rev=cscc6&num=201503&vol=3&aid=4301conventional bioreactorEcoli BL21recombinant proteinSakaguchi flaskshrimp vaccine candidate |
| spellingShingle | MUHAMAD ALI ISMAINI SULAIMAN N. DEPAMEDE BAGUS D. H. SETYONO ALIS MUKHLIS MUHAMAD AMIN MOHAMMAD ASHARI STIRRED BIOREACTOR FOR THE ROBUSTNESS PRODUCTION OF RECOMBINANT GST.VP28 IN FED-BATCH CULTIVATION OF ESCHERICHIA COLI Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry conventional bioreactor E coli BL21 recombinant protein Sakaguchi flask shrimp vaccine candidate |
| title | STIRRED BIOREACTOR FOR THE ROBUSTNESS PRODUCTION OF RECOMBINANT GST.VP28 IN FED-BATCH CULTIVATION OF ESCHERICHIA COLI |
| title_full | STIRRED BIOREACTOR FOR THE ROBUSTNESS PRODUCTION OF RECOMBINANT GST.VP28 IN FED-BATCH CULTIVATION OF ESCHERICHIA COLI |
| title_fullStr | STIRRED BIOREACTOR FOR THE ROBUSTNESS PRODUCTION OF RECOMBINANT GST.VP28 IN FED-BATCH CULTIVATION OF ESCHERICHIA COLI |
| title_full_unstemmed | STIRRED BIOREACTOR FOR THE ROBUSTNESS PRODUCTION OF RECOMBINANT GST.VP28 IN FED-BATCH CULTIVATION OF ESCHERICHIA COLI |
| title_short | STIRRED BIOREACTOR FOR THE ROBUSTNESS PRODUCTION OF RECOMBINANT GST.VP28 IN FED-BATCH CULTIVATION OF ESCHERICHIA COLI |
| title_sort | stirred bioreactor for the robustness production of recombinant gst vp28 in fed batch cultivation of escherichia coli |
| topic | conventional bioreactor E coli BL21 recombinant protein Sakaguchi flask shrimp vaccine candidate |
| url | http://pubs.ub.ro/?pg=revues&rev=cscc6&num=201503&vol=3&aid=4301 |
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