Blocking transmission of Middle East respiratory syndrome coronavirus (MERS-CoV) in llamas by vaccination with a recombinant spike protein
The ongoing Middle East respiratory syndrome coronavirus (MERS-CoV) outbreaks pose a worldwide public health threat. Blocking MERS-CoV zoonotic transmission from dromedary camels, the animal reservoir, could potentially reduce the number of primary human cases. Here we report MERS-CoV transmission f...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | Emerging Microbes and Infections |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2019.1685912 |
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| author | Jordi Rodon Nisreen M. A. Okba Nigeer Te Brenda van Dieren Berend-Jan Bosch Albert Bensaid Joaquim Segalés Bart L. Haagmans Júlia Vergara-Alert |
| author_facet | Jordi Rodon Nisreen M. A. Okba Nigeer Te Brenda van Dieren Berend-Jan Bosch Albert Bensaid Joaquim Segalés Bart L. Haagmans Júlia Vergara-Alert |
| author_sort | Jordi Rodon |
| collection | DOAJ |
| description | The ongoing Middle East respiratory syndrome coronavirus (MERS-CoV) outbreaks pose a worldwide public health threat. Blocking MERS-CoV zoonotic transmission from dromedary camels, the animal reservoir, could potentially reduce the number of primary human cases. Here we report MERS-CoV transmission from experimentally infected llamas to naïve animals. Directly inoculated llamas shed virus for at least 6 days and could infect all in-contact naïve animals 4–5 days after exposure. With the aim to block virus transmission, we examined the efficacy of a recombinant spike S1-protein vaccine. In contrast to naïve animals, in-contact vaccinated llamas did not shed infectious virus upon exposure to directly inoculated llamas, consistent with the induction of strong virus neutralizing antibody responses. Our data provide further evidence that vaccination of the reservoir host may impede MERS-CoV zoonotic transmission to humans. |
| format | Article |
| id | doaj-art-c9d80acfca5e4c13999977a662bc76c8 |
| institution | DOAJ |
| issn | 2222-1751 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-c9d80acfca5e4c13999977a662bc76c82025-08-20T03:17:54ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512019-01-01811593160310.1080/22221751.2019.1685912Blocking transmission of Middle East respiratory syndrome coronavirus (MERS-CoV) in llamas by vaccination with a recombinant spike proteinJordi Rodon0Nisreen M. A. Okba1Nigeer Te2Brenda van Dieren3Berend-Jan Bosch4Albert Bensaid5Joaquim Segalés6Bart L. Haagmans7Júlia Vergara-Alert8IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Bellaterra (Cerdanyola del Vallès), SpainDepartment of Viroscience, Erasmus Medical Centre, Rotterdam, The NetherlandsIRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Bellaterra (Cerdanyola del Vallès), SpainVirology Division, Department of Infectious Diseases & Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The NetherlandsVirology Division, Department of Infectious Diseases & Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The NetherlandsIRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Bellaterra (Cerdanyola del Vallès), SpainUAB, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Bellaterra (Cerdanyola del Vallès), SpainDepartment of Viroscience, Erasmus Medical Centre, Rotterdam, The NetherlandsIRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Bellaterra (Cerdanyola del Vallès), SpainThe ongoing Middle East respiratory syndrome coronavirus (MERS-CoV) outbreaks pose a worldwide public health threat. Blocking MERS-CoV zoonotic transmission from dromedary camels, the animal reservoir, could potentially reduce the number of primary human cases. Here we report MERS-CoV transmission from experimentally infected llamas to naïve animals. Directly inoculated llamas shed virus for at least 6 days and could infect all in-contact naïve animals 4–5 days after exposure. With the aim to block virus transmission, we examined the efficacy of a recombinant spike S1-protein vaccine. In contrast to naïve animals, in-contact vaccinated llamas did not shed infectious virus upon exposure to directly inoculated llamas, consistent with the induction of strong virus neutralizing antibody responses. Our data provide further evidence that vaccination of the reservoir host may impede MERS-CoV zoonotic transmission to humans.https://www.tandfonline.com/doi/10.1080/22221751.2019.1685912Animal modelllamaMiddle East respiratory syndrome coronavirusMERS-CoVS1-protein-based vaccinevirus transmission |
| spellingShingle | Jordi Rodon Nisreen M. A. Okba Nigeer Te Brenda van Dieren Berend-Jan Bosch Albert Bensaid Joaquim Segalés Bart L. Haagmans Júlia Vergara-Alert Blocking transmission of Middle East respiratory syndrome coronavirus (MERS-CoV) in llamas by vaccination with a recombinant spike protein Emerging Microbes and Infections Animal model llama Middle East respiratory syndrome coronavirus MERS-CoV S1-protein-based vaccine virus transmission |
| title | Blocking transmission of Middle East respiratory syndrome coronavirus (MERS-CoV) in llamas by vaccination with a recombinant spike protein |
| title_full | Blocking transmission of Middle East respiratory syndrome coronavirus (MERS-CoV) in llamas by vaccination with a recombinant spike protein |
| title_fullStr | Blocking transmission of Middle East respiratory syndrome coronavirus (MERS-CoV) in llamas by vaccination with a recombinant spike protein |
| title_full_unstemmed | Blocking transmission of Middle East respiratory syndrome coronavirus (MERS-CoV) in llamas by vaccination with a recombinant spike protein |
| title_short | Blocking transmission of Middle East respiratory syndrome coronavirus (MERS-CoV) in llamas by vaccination with a recombinant spike protein |
| title_sort | blocking transmission of middle east respiratory syndrome coronavirus mers cov in llamas by vaccination with a recombinant spike protein |
| topic | Animal model llama Middle East respiratory syndrome coronavirus MERS-CoV S1-protein-based vaccine virus transmission |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2019.1685912 |
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