Platelet rich plasma: Effective treatment for repairing of spinal cord injury in rat

Objective: The aim of the present study was to evaluate the effect of PRP on the repair of spinal cord injury in rat model. Material and methods: Rats were randomly divided into three groups with six rats in each group. Then, spinal cord injury was performed under general anesthesia using “weight dr...

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Main Authors: Reza Salarinia, Hamid Reza Sadeghnia, Daryoush Hamidi Alamdari, Seyed Javad Hoseini, Asghar Mafinezhad, Mahmoud Hosseini
Format: Article
Language:English
Published: AVES 2017-05-01
Series:Acta Orthopaedica et Traumatologica Turcica
Online Access:http://www.sciencedirect.com/science/article/pii/S1017995X17301001
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author Reza Salarinia
Hamid Reza Sadeghnia
Daryoush Hamidi Alamdari
Seyed Javad Hoseini
Asghar Mafinezhad
Mahmoud Hosseini
author_facet Reza Salarinia
Hamid Reza Sadeghnia
Daryoush Hamidi Alamdari
Seyed Javad Hoseini
Asghar Mafinezhad
Mahmoud Hosseini
author_sort Reza Salarinia
collection DOAJ
description Objective: The aim of the present study was to evaluate the effect of PRP on the repair of spinal cord injury in rat model. Material and methods: Rats were randomly divided into three groups with six rats in each group. Then, spinal cord injury was performed under general anesthesia using “weight dropping” method. Control group included rats receiving normal saline, group two received PRP 1 week after injury; group three received PRP 24 h after injury. The motor function was assessed weekly using the Basso, Beattie, and Bresnahan (BBB) locomotor rating scale. Anterograde tracing was performed for evaluation of axon regeneration. Result: Motor recovery was significantly better in the rats treated with PRP 24 h after injury than the control group. In the rats treated with PRP 1 week after injury and rats treated with PRP 24 h after injury, the average numbers of BDA-labeled axons were statistically different from the control group. Conclusion: Our experimental study demonstrated positive effects of platelet rich plasma on nerve regeneration after spinal cord injury. Keywords: Spinal cord injury, Platelet rich plasma, Locomotor function
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institution Kabale University
issn 1017-995X
language English
publishDate 2017-05-01
publisher AVES
record_format Article
series Acta Orthopaedica et Traumatologica Turcica
spelling doaj-art-c9d624fb4cea431c9d4ab593aefc5c0e2025-08-20T03:36:06ZengAVESActa Orthopaedica et Traumatologica Turcica1017-995X2017-05-0151325425710.1016/j.aott.2017.02.009Platelet rich plasma: Effective treatment for repairing of spinal cord injury in ratReza Salarinia0Hamid Reza Sadeghnia1Daryoush Hamidi Alamdari2Seyed Javad Hoseini3Asghar Mafinezhad4Mahmoud Hosseini5Department of Medical Biotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Medical Biotechnology and Molecular Sciences, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, IranPharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; Corresponding author.Biochemistry and Nutrition Research Center, Department of Clinical Biochemistry, School of Medicine, Mashhad University of Medical Sciences, IranDepartment of Medical Biotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranPathology Department of Shahid Kamyab Hospital, Mashhad University of Medical Sciences, Mashhad, IranDivision of Neurocognitive Sciences, Psychiatry and Behavioral Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, IranObjective: The aim of the present study was to evaluate the effect of PRP on the repair of spinal cord injury in rat model. Material and methods: Rats were randomly divided into three groups with six rats in each group. Then, spinal cord injury was performed under general anesthesia using “weight dropping” method. Control group included rats receiving normal saline, group two received PRP 1 week after injury; group three received PRP 24 h after injury. The motor function was assessed weekly using the Basso, Beattie, and Bresnahan (BBB) locomotor rating scale. Anterograde tracing was performed for evaluation of axon regeneration. Result: Motor recovery was significantly better in the rats treated with PRP 24 h after injury than the control group. In the rats treated with PRP 1 week after injury and rats treated with PRP 24 h after injury, the average numbers of BDA-labeled axons were statistically different from the control group. Conclusion: Our experimental study demonstrated positive effects of platelet rich plasma on nerve regeneration after spinal cord injury. Keywords: Spinal cord injury, Platelet rich plasma, Locomotor functionhttp://www.sciencedirect.com/science/article/pii/S1017995X17301001
spellingShingle Reza Salarinia
Hamid Reza Sadeghnia
Daryoush Hamidi Alamdari
Seyed Javad Hoseini
Asghar Mafinezhad
Mahmoud Hosseini
Platelet rich plasma: Effective treatment for repairing of spinal cord injury in rat
Acta Orthopaedica et Traumatologica Turcica
title Platelet rich plasma: Effective treatment for repairing of spinal cord injury in rat
title_full Platelet rich plasma: Effective treatment for repairing of spinal cord injury in rat
title_fullStr Platelet rich plasma: Effective treatment for repairing of spinal cord injury in rat
title_full_unstemmed Platelet rich plasma: Effective treatment for repairing of spinal cord injury in rat
title_short Platelet rich plasma: Effective treatment for repairing of spinal cord injury in rat
title_sort platelet rich plasma effective treatment for repairing of spinal cord injury in rat
url http://www.sciencedirect.com/science/article/pii/S1017995X17301001
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AT daryoushhamidialamdari plateletrichplasmaeffectivetreatmentforrepairingofspinalcordinjuryinrat
AT seyedjavadhoseini plateletrichplasmaeffectivetreatmentforrepairingofspinalcordinjuryinrat
AT asgharmafinezhad plateletrichplasmaeffectivetreatmentforrepairingofspinalcordinjuryinrat
AT mahmoudhosseini plateletrichplasmaeffectivetreatmentforrepairingofspinalcordinjuryinrat