Effect of P Reduction on <i>phoD</i>-Harboring Bacteria Community in Solar Greenhouse Soil
Phosphorus (P) enrichment frequently occurs in the soil used in greenhouse vegetable production (GVP). Minimizing the application of P fertilizer represents a crucial approach to mitigating the accumulation of P in the soil and enhancing its utilization efficiency. However, the changes in bacterial...
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2024-10-01
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| author | Ting Bian Zhen Wang Shuang Wang Xuan Shan Tianqi Wang Hongdan Fu Zhouping Sun |
| author_facet | Ting Bian Zhen Wang Shuang Wang Xuan Shan Tianqi Wang Hongdan Fu Zhouping Sun |
| author_sort | Ting Bian |
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| description | Phosphorus (P) enrichment frequently occurs in the soil used in greenhouse vegetable production (GVP). Minimizing the application of P fertilizer represents a crucial approach to mitigating the accumulation of P in the soil and enhancing its utilization efficiency. However, the changes in bacterial communities and the turnover mechanism of soil P fractions related to soil P cycling after P fertilizer reduction are still unclear. To unravel these complexities, we devised three experimental treatments: conventional nitrogen (N), P, and potassium (K) fertilizer (N1P1K1); conventional N and K fertilizer without P (N1P0K1); and no fertilizer (N0P0K0). These experiments were conducted to elucidate the effects of P reduction on cucumber plant growth, soil P fractions, and the <i>phoD</i>-harboring bacterial community in the P-rich greenhouse soil. The results showed that there were no significant differences between the N1P1K1 and N1P0K1 treatments in terms of plant growth, yield, and P uptake, and the values for the N0P0K0 treatment were significantly lower than those for the N1P1K1 treatment. In a state of P depletion (N0P0K0, N1P0K1), the main P sources were Resin-P<sub>i</sub>, NaHCO<sub>3</sub>-P<sub>i</sub>, NaHCO<sub>3</sub>-P<sub>o</sub>, and NaOH-P<sub>i</sub>. The contents of NaOH-P<sub>o</sub> and CHCl-P<sub>o</sub> in the N1P0K1 treatment increased significantly. Without P fertilizer, alkaline phosphatase (ALP) activity, <i>phoD</i> gene abundance, and bacterial community diversity were significantly increased. The abundance of <i>Ensifer</i> in the N0P0K0 and N1P0K1 treatments was 8 and 10.58 times that in the N1P1K1 treatment, respectively. Additionally, total phosphorus (TP) and available nitrogen (AN) were key factors affecting changes in the <i>phoD</i> bacterial community, while <i>Shinella</i>, <i>Ensifer</i> and <i>Bradyrhizobium</i> were the main factors driving the change in soil P fractions, and NaHCO<sub>3</sub>-P<sub>i</sub> and NaOH-P<sub>i</sub> were key factors affecting crop yield. Therefore, reducing the application of P fertilizer will increases the diversity of <i>phoD</i>-gene-harboring bacterial communities and promote organic P mineralization, thus maintaining the optimal crop yield. |
| format | Article |
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| spelling | doaj-art-c9d37793e4eb43e89316fc6dabe8340b2025-08-20T02:26:50ZengMDPI AGAgriculture2077-04722024-10-011411191910.3390/agriculture14111919Effect of P Reduction on <i>phoD</i>-Harboring Bacteria Community in Solar Greenhouse SoilTing Bian0Zhen Wang1Shuang Wang2Xuan Shan3Tianqi Wang4Hongdan Fu5Zhouping Sun6College of Horticulture, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Horticulture, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Horticulture, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Horticulture, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Horticulture, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Horticulture, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Horticulture, Shenyang Agricultural University, Shenyang 110866, ChinaPhosphorus (P) enrichment frequently occurs in the soil used in greenhouse vegetable production (GVP). Minimizing the application of P fertilizer represents a crucial approach to mitigating the accumulation of P in the soil and enhancing its utilization efficiency. However, the changes in bacterial communities and the turnover mechanism of soil P fractions related to soil P cycling after P fertilizer reduction are still unclear. To unravel these complexities, we devised three experimental treatments: conventional nitrogen (N), P, and potassium (K) fertilizer (N1P1K1); conventional N and K fertilizer without P (N1P0K1); and no fertilizer (N0P0K0). These experiments were conducted to elucidate the effects of P reduction on cucumber plant growth, soil P fractions, and the <i>phoD</i>-harboring bacterial community in the P-rich greenhouse soil. The results showed that there were no significant differences between the N1P1K1 and N1P0K1 treatments in terms of plant growth, yield, and P uptake, and the values for the N0P0K0 treatment were significantly lower than those for the N1P1K1 treatment. In a state of P depletion (N0P0K0, N1P0K1), the main P sources were Resin-P<sub>i</sub>, NaHCO<sub>3</sub>-P<sub>i</sub>, NaHCO<sub>3</sub>-P<sub>o</sub>, and NaOH-P<sub>i</sub>. The contents of NaOH-P<sub>o</sub> and CHCl-P<sub>o</sub> in the N1P0K1 treatment increased significantly. Without P fertilizer, alkaline phosphatase (ALP) activity, <i>phoD</i> gene abundance, and bacterial community diversity were significantly increased. The abundance of <i>Ensifer</i> in the N0P0K0 and N1P0K1 treatments was 8 and 10.58 times that in the N1P1K1 treatment, respectively. Additionally, total phosphorus (TP) and available nitrogen (AN) were key factors affecting changes in the <i>phoD</i> bacterial community, while <i>Shinella</i>, <i>Ensifer</i> and <i>Bradyrhizobium</i> were the main factors driving the change in soil P fractions, and NaHCO<sub>3</sub>-P<sub>i</sub> and NaOH-P<sub>i</sub> were key factors affecting crop yield. Therefore, reducing the application of P fertilizer will increases the diversity of <i>phoD</i>-gene-harboring bacterial communities and promote organic P mineralization, thus maintaining the optimal crop yield.https://www.mdpi.com/2077-0472/14/11/1919cucumbergreenhouse soilsoil P fractions<i>phoD</i>-harboring bacteria |
| spellingShingle | Ting Bian Zhen Wang Shuang Wang Xuan Shan Tianqi Wang Hongdan Fu Zhouping Sun Effect of P Reduction on <i>phoD</i>-Harboring Bacteria Community in Solar Greenhouse Soil Agriculture cucumber greenhouse soil soil P fractions <i>phoD</i>-harboring bacteria |
| title | Effect of P Reduction on <i>phoD</i>-Harboring Bacteria Community in Solar Greenhouse Soil |
| title_full | Effect of P Reduction on <i>phoD</i>-Harboring Bacteria Community in Solar Greenhouse Soil |
| title_fullStr | Effect of P Reduction on <i>phoD</i>-Harboring Bacteria Community in Solar Greenhouse Soil |
| title_full_unstemmed | Effect of P Reduction on <i>phoD</i>-Harboring Bacteria Community in Solar Greenhouse Soil |
| title_short | Effect of P Reduction on <i>phoD</i>-Harboring Bacteria Community in Solar Greenhouse Soil |
| title_sort | effect of p reduction on i phod i harboring bacteria community in solar greenhouse soil |
| topic | cucumber greenhouse soil soil P fractions <i>phoD</i>-harboring bacteria |
| url | https://www.mdpi.com/2077-0472/14/11/1919 |
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